Isoelectronic focusing electrophoretic device for inspecting polypeptide agonist
An isoelectric focusing electrophoresis and stimulant technology, which is used in measurement devices, biological tests, material inspection products, etc., can solve the problems of inability to use peptide stimulant detection, short half-life of polypeptide stimulants, unstable pH gradient, etc. The effect of shortening the analysis and detection time, solving the detection sensitivity, and improving the detection sensitivity
Active Publication Date: 2009-09-02
SHANGHAI JIAODA INTELLECTUAL PORPERTY MANAGEMENT CO LTD +1
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Problems solved by technology
However, due to the absence of an online enrichment method for polypeptide stimulants, the instability of the pH gradient and the long time for gel isoelectric focusing electrophoresis-electrotransfer-chemiluminescence in IEF, and the short half-life of polypeptide stimulants And the content in urine is very low, the device and method of Breidbach cannot be applied to the actual detection of polypeptide doping agents
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[0018] like figure 1 As shown, the present invention includes: anode 1, anode tube 2, anode sample tube 3, no outer coating quartz capillary tube 4, cathode sample tube 5, cathode tube 6, cathode 7, diaphragm 8, DC power supply 9, electrode wire 10, Operation window 11, doors and windows 12, operation platform 13, support column 14, box base 15, box body 16, box top 17, ultraviolet lamp 18, ultraviolet reflector 19, CCD digital imager 21, computer 22, data line 23. The connection relationship is: the anode 1 and the cathode 7 are respectively connected to the positive pole and the negative pole of the DC power supply 9 through the electrode wire 10, the anode 1 and the cathode 7 are respectively placed in the anode tube 2 and the cathode tube 6, and the anode tube 2 and the cathode tube 6 are connected through the diaphragm 8 are respectively connected with the anode sample tube 3 and the cathode sample tube 5, and the two ends of the uncoated quartz capillary 4 are respective...
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Abstract
An isoelectric focusing electrophoresis device for detecting polypeptide doping agents in the field of detection technology. In the present invention, the anode and the cathode are connected to the positive pole and the negative pole of the DC power supply, the anode and the cathode are placed in the anode tube and the cathode tube, and the anode tube and the cathode tube are respectively connected to the anode sample tube and the cathode sample tube through a diaphragm, without outer coating The two ends of the quartz capillary are respectively connected to the anode sample tube and the cathode sample tube; the operation platform is connected to the base of the box, the base of the box is connected to the box, the upper part of the box is connected to the top of the box, and there is an operation window on the box. Connect doors and windows; the ultraviolet lamp is placed on both sides of the quartz capillary without outer coating, and a UV reflector is arranged on the upper side of the ultraviolet lamp, and the ultraviolet light is evenly irradiated on the quartz capillary without outer coating during imaging; the CCD digital imager is connected to On the top of the box, the CCD digital imager is connected with the computer through a data line. The invention is used in the detection and control of polypeptide stimulants in competitive sports events, and is fast, stable and sensitive.
Description
technical field [0001] The present invention relates to a device in the technical field of detection, in particular to an isoelectric focusing electrophoresis device for detecting polypeptide stimulants. Background technique [0002] Growth hormone (hGH) and erythropoietin (hEPO) are endogenous sex hormones in the human body. Due to the ability to significantly improve sports performance, the abuse of this type of polypeptide stimulants (especially recombinant r-hGH and r-hEPO) is on the rise all over the world. At present, there are mainly two types of methods for the screening of r-hGH and rhEPO abuse, namely indirect methods and direct methods. There are many types of indirect methods, which can provide preliminary screening basis, but cannot provide decisive screening basis. Direct detection methods can provide a decisive basis for discrimination. [0003] Find through literature search to prior art: Breidbach etc. have published " Detection of Recombinant Human Eryth...
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IPC IPC(8): G01N33/68G01N27/447
Inventor 曹成喜张薇樊柳荫徐研杰
Owner SHANGHAI JIAODA INTELLECTUAL PORPERTY MANAGEMENT CO LTD