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Electrode material for aptamer sensor, electrochemical aptamer sensor and preparation method of electrochemical aptamer sensor

An aptamer sensor and electrode material technology, which is applied in the direction of material electrochemical variables, material analysis by electromagnetic means, and material analysis, can solve the problem of small amount of aptamer, low detection sensitivity of electrochemical aptasensor, and inability to effectively improve electrical Chemical aptamer sensor detection sensitivity and other issues, to achieve the effect of low detection limit, excellent selectivity, and good reproducibility

Active Publication Date: 2019-08-16
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an electrode material for an aptamer sensor, to solve the problem that the amount of aptamer fixed by the existing electrode material is small, and the detection sensitivity of the electrochemical aptasensor cannot be effectively improved.
[0007] The second object of the present invention is to provide an electrochemical aptasensor to solve the problem of low detection sensitivity of the existing electrochemical aptasensor
[0008] The third object of the present invention is to provide a method for preparing an electrochemical aptasensor to solve the problem of low detection sensitivity of the electrochemical aptasensor

Method used

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  • Electrode material for aptamer sensor, electrochemical aptamer sensor and preparation method of electrochemical aptamer sensor
  • Electrode material for aptamer sensor, electrochemical aptamer sensor and preparation method of electrochemical aptamer sensor
  • Electrode material for aptamer sensor, electrochemical aptamer sensor and preparation method of electrochemical aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The electrode material for the aptasensor of the present embodiment comprises the following steps:

[0070] 1) Synthesis of CoFe-PBA: CoCl 2 ·6H 2 O (142.8mg), PVP (0.3g) and sodium citrate (0.25g) were dissolved in 30mL deionized water to form the first solution; 3 [Fe(CN) 6 ] (66 mg) was dissolved in 20 mL of deionized water to form a second solution. Then, the second solution was added dropwise into the first solution under magnetic stirring, and after 10 minutes, the reaction system was transferred and left to stand for 24 hours. Finally, the precipitate was washed three times with ethanol and dried at 60 °C to obtain CoFe-PBA.

[0071] 2) Preparation of CoNi-ZIF@CoFe-PBA: Co(NO 3 ) 2 ·6H 2 O(0.55g) and Ni(NO 3 ) 2 ·6H 2 O (0.55g) was dissolved in 30mL of methanol under magnetic stirring to form solution A; 2-methylimidazole (1.32g), CoFe-PBA (100mg) were dispersed in 30mL of methanol under magnetic stirring to form solution B, Add solution A to solution ...

Embodiment 2-3

[0075] The preparation steps of the electrode material for the aptamer sensor in Example 2-3 are basically the same as that of the electrode material in Example 1, the only difference is that in step 3), the calcination temperatures are 600°C and 900°C, respectively, to obtain 600°C The calcined product and the calcined product at 900°C are respectively expressed as NiO / Fe 2 o 3 / NiCo 2 o 4,600 and NiO / Fe 2 o 3 / NiCo 2 o 4,900 .

[0076] Two, the specific embodiment of the preparation method of the electrochemical aptamer sensor of the present invention is as follows:

Embodiment 4

[0078] The preparation method of the electrochemical aptasensor of this embodiment comprises the following steps:

[0079] 1) Pretreatment of bare gold electrodes (AE): Polish the AE surface with 0.05 μm alumina and rinse with deionized water. Then soak AE in H with a volume ratio of 7:3 2 SO 4 :H 2 o 2 The solution was sonicated for 15 min, washed with ultrapure water, and dried under nitrogen. Finally, at 0.5M H 2 SO 4 Electrochemical scanning is used in the process, and cyclic voltammetry is used to test in the range of -0.2-1.6V to complete electrochemical cleaning.

[0080] 2) Construction of electrochemical aptasensor: 1.0 mg of NiO / Fe 2 o 3 / NiCo 2 o 4,300 Disperse in 1.0 mL of ultrapure water to form a uniform dispersion to obtain a dispersion with a concentration of 1.0 mg / mL. Add 5 μL of the dispersion solution dropwise to the AE surface after electrochemical cleaning in step 1), and dry it at room temperature for 1 h to obtain a modified electrode (expres...

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Abstract

The invention relates to an electrode material for an aptamer sensor, an electrochemical aptamer sensor and a preparation method of the electrochemical aptamer sensor. The electrode material for the aptamer sensor is prepared from the following steps that 1) a cobalt salt and ferricyanate are reacted in a solvent to prepare a solid-phase cobalt-iron complex; 2) the cobalt-iron complex, the cobaltsalt, a nickel salt and 2-methylimidazole are stirred and reacted in a solvent to prepare a solid-phase coating material; and 3) the solid-phase coating material is subjected to calcining treatment under protective atmosphere at 300 DEG C or above to prepare the electrode material. According to the electrode material for the aptamer sensor, due to an inherent cavity of a framework and the synergistic effect between different metal oxides and NiCo2O4 spinel, the electrode material can be used for fixing a large amount of aptamer chains, so that the detection performance of the corresponding electrochemical aptamer sensor can be improved.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, in particular to an electrode material for an aptamer sensor, an electrochemical aptamer sensor and a preparation method thereof. Background technique [0002] Accurate detection of insulin levels in the blood has important implications for the care of diabetic patients. So far, several analytical methods for insulin detection have been developed, including capillary electrophoresis, high-performance liquid chromatography, electrochemical detection, fluorescence detection, and surface plasmon resonance. Even though many efforts have been made, there are still some disadvantages of traditional analytical methods, such as cumbersome, time-consuming and expensive, which limit the rapid detection of insulin. [0003] As single-stranded DNA or RNA molecules, aptamers can specifically recognize and bind small molecules or even whole cells through three-dimensional folding. Compared with antibod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3275G01N27/3277G01N27/3278G01N27/48
Inventor 张治红王明花何领好宋英攀崔静刘永康黄顺江
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY