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Organic selenium from aspergillus oryzae and method for preparing same

A technology for Aspergillus oryzae and organic selenium, which is applied in the field of organic selenium and its preparation, and achieves the effects of disease control, high organic selenium content and strong selenium enrichment ability

Inactive Publication Date: 2009-10-14
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic selenium derived from Aspergillus oryzae provides people with an organic selenium salt that is easier to absorb and utilize than inorganic selenium salts, overcoming the shortcomings of direct supplementation of inorganic selenium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] First prepare the liquid bean sprouts juice culture medium. Then prepare the sodium selenite mother liquor of 10g / L, absorb a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 150mg / L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, and cultivate them at 37°C and 150rpm for 24 hours as the primary seed liquid; Sodium bean sprouts juice culture medium, 37 ℃, 150rpm culture 24h, as secondary seed liquid; The secondary seed liquid is inoculated in the growth medium according to the inoculum amount of 10%, and the growth medium also contains sodium selenite bean sprouts juice The culture medium was cultured at 37° C. and 150 rpm for 60 hours, and centrifuged at 8000 rpm for 30 minutes to collect Aspergillus oryzae cells. Wash with normal saline for 3 times to remove extracellular selenium compoun...

Embodiment 2

[0043] First prepare the liquid bean juice culture medium. Prepare the sodium selenite mother liquor of 5g / L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 100mg / L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, cultivate them for 20 hours at 37°C and 130 rpm, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth medium according to the inoculum amount of 10%. Among them, the growth medium also contains sodium selenite bean sprouts juice medium, 30 ℃, 130rpm, culture 90h, 8 layers of gauze filter to collect Aspergillus oryzae cells. Wash 12 times with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 3%, autolyze for 24 hours at 55°C, filter with filter paper to remove insoluble ma...

Embodiment 3

[0045] First prepare the liquid bean juice culture medium. Prepare the sodium selenite mother liquor of 50mg / L again, draw a certain amount of mother liquor and join in the bean sprouts juice culture medium, make the final concentration of sodium selenite in the culture medium be 10mg / L. Pick Aspergillus oryzae spores and directly inoculate them into the bean sprouts juice medium containing sodium selenite, cultivate them at 37°C and 100 rpm for 20 hours, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth medium according to the inoculum amount of 10%. Among them, the growth medium also contains sodium selenite bean sprouts juice medium, 37 ° C, 100 rpm, cultivated for 48 hours, and collected Aspergillus oryzae cells by filtering through 8 layers of gauze. Wash twice with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 2.5%, autolyze at 50°C for 30 hours, filter with fil...

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PUM

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Abstract

The organic selenium derived from aspergillus oryzae and a preparation method thereof of the present invention belong to the field of food and health product additives. The organic selenium of the present invention uses Aspergillus oryzae as a carrier, and exists in the form of Aspergillus oryzae cells, aqueous solution or dry powder. Its preparation method uses selenium compound as raw material, through the process of preparing selenium-containing medium, inoculating and culturing Aspergillus oryzae, collecting Aspergillus oryzae, washing to remove extracellular selenium, sterilizing or / and drying; the organic aspergillus oryzae cell form is obtained by drying. Selenium, or the organic selenium in the form of an aqueous solution obtained from thalline autolysis, or the organic selenium in the form of dry powder obtained by drying the aqueous solution. Aspergillus oryzae has the characteristics of fast growth and high content of organic selenium in selenium-enriched Aspergillus oryzae cells. During the fermentation process, Aspergillus oryzae can convert inorganic selenium into organic selenium, which is easily metabolized and absorbed by the human body. Using Aspergillus oryzae containing organic selenium instead of common Aspergillus oryzae as an additive in food and health products can supplement selenium in a small amount and continuously, and effectively prevent and treat diseases caused by selenium deficiency.

Description

technical field [0001] The invention belongs to the field of human food additives, and relates to organic selenium derived from aspergillus oryzae and a preparation method thereof. Background technique [0002] Selenium exists in various functional proteases in the body. It is a key component element of glutathione peroxidase, phospholipid hydrogen glutathione peroxidase, and 5'-deiodinase active center, and participates in the metabolism of peroxide and iodine in the body. Selenium has the ability to scavenge free radicals, Preventing cancer, anti-aging and beautifying, protecting the liver, resisting harmful heavy metals, anti-radiation, regulating immune function, etc. Selenium can obviously promote the recovery of hepatitis, cardiovascular disease, and adjuvant treatment of radiotherapy and chemotherapy for tumor patients. In 1973, the World Health Organization (WHO) believed that selenium is an essential trace element in human and animal life, and selenium supplementat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00C12P9/00C12N1/14A23L1/304A61P39/06A23L33/16
Inventor 逯家辉滕利荣高朝辉孟庆繁陈亚光王贞佐
Owner JILIN UNIV
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