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Organic selenium from aspergillus niger and preparation method thereof

A technology of organic selenium and Aspergillus niger, which is applied in food preparation, application, food science and other directions to achieve the effects of fast growth of bacteria, high organic selenium content and strong selenium enrichment ability

Inactive Publication Date: 2010-11-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic selenium derived from Aspergillus niger provides people with an organic selenium salt that is easier to absorb and utilize than inorganic selenium salts, overcoming the shortcomings of direct supplementation of inorganic selenium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] First prepare the liquid bran juice medium. Prepare the sodium selenite mother liquor of 10g / L again, draw a certain amount of mother liquor and join in the bran juice culture medium, make the final concentration of sodium selenite in the culture medium be 150mg / L. Pick Aspergillus niger spores and directly inoculate them into the bran juice medium containing sodium selenite, cultivate them at 37°C and 150rpm for 24 hours, and use them as the primary seed liquid; In the bran juice culture medium of sodium selenite, culture 24h at 37 ℃, 150rpm, as the secondary seed solution; inoculate the secondary seed solution into the growth medium according to the inoculation amount of 10%, and the growth medium also contains sodium selenite Bran juice culture medium, cultured at 37°C, 150rpm for 60h, centrifuged at 8000rpm for 30min to collect Aspergillus niger cells. Wash with normal saline for 3 times to remove extracellular selenium compounds, and then sterilize with damp heat ...

Embodiment 2

[0042] First prepare the liquid bran juice medium. Prepare the sodium selenite mother liquor of 5g / L again, draw a certain amount of mother liquor and join in the bran juice culture medium, make the final concentration of sodium selenite in the culture medium be 100mg / L. Pick Aspergillus niger spores and directly inoculate them into the bran juice medium containing sodium selenite, cultivate them for 20 hours at 37°C and 130 rpm, and use them as the primary seed liquid; inoculate the primary seed liquid into the growth culture medium according to the inoculation amount of 10%. In the base, the growth medium also contains sodium selenite bran juice medium, 30°C, 130rpm, cultivated for 90h, and collected Aspergillus niger cells by filtering through 8 layers of gauze. Wash 12 times with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 3%, autolyze for 24 hours at 55°C, filter with filter paper to remove insoluble ...

Embodiment 3

[0044] First prepare the liquid bran juice medium. Prepare the sodium selenite mother liquor of 50mg / L again, draw a certain amount of mother liquor and join in the bran juice culture medium, make the final concentration of sodium selenite in the culture medium be 10mg / L. Pick Aspergillus niger spores and directly inoculate them into the bran juice medium containing sodium selenite, cultivate them for 20 hours at 37°C and 100 rpm, and use them as the first-level seed solution; inoculate the first-level seed solution into the growth culture medium according to the inoculation amount of 10%. In the base, the growth medium also contains sodium selenite bran juice medium, 37°C, 100rpm, cultivated for 48h, and collected Aspergillus niger cells by filtering through 8 layers of gauze. Wash twice with aqueous glucose solution to remove extracellular selenium compounds, add solid NaCl until the concentration reaches 2.5%, autolyze at 50°C for 30 hours, filter with filter paper to remov...

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Abstract

The invention relates to a kind of organic selenium from aspergillus niger and preparation method, wherein the organic selenium uses aspergillus niger as carrying agent and exists in the form of aspergillus niger cell or aqueous solution or dry-type powder. The process for preparing comprises using selenium compound as raw material, preparing selenium-containing culture medium, vaccination culturing aspergillus niger, collection of aspergillus niger, washing to expel the selenium out of the cells, sterilizing or / and drying.

Description

technical field [0001] The invention belongs to the field of human food additives, and relates to organic selenium derived from Aspergillus niger and a preparation method thereof. Background technique [0002] Selenium exists in various functional proteases in the body. It is a key component element of glutathione peroxidase, phospholipid hydrogen glutathione peroxidase, and 5'-deiodinase active center, and participates in the metabolism of peroxide and iodine in the body. Selenium has the ability to scavenge free radicals, Preventing cancer, anti-aging and beautifying, protecting the liver, resisting harmful heavy metals, anti-radiation, regulating immune function, etc. Selenium can obviously promote the recovery of hepatitis, cardiovascular disease, and adjuvant treatment of radiotherapy and chemotherapy for tumor patients. In 1973, the World Health Organization (WHO) believed that selenium is an essential trace element in human and animal life, and selenium supplementati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/30A23L31/00A23L33/16
Inventor 滕利荣高朝辉孟庆繁逯家辉程瑛琨蒋朝军
Owner JILIN UNIV
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