Refolding method for protein in electrostrictive polymer

Abstract

The invention relates to an analysis method of protein refolding in electrical contraction polymer. The invention utilizes the crosslink network of electrical contraction polymer to insulate modifiedprotein molecule and removes modifier and contracts polymer at electric field, to loosen the polymer packing modified protein, therefore, modifier protein can fold without interfered and coiled by other proteins, and without the generation of inclusion body. The inventive analysis method builds a model of external molecule mate, for protein folding dynamic research.

Description

【Technical field】:

[0001] The invention relates to the field of protein folding research, and provides an experimental method for protein refolding under the condition of mutual non-interference. 【Background technique】:

[0002] Protein folding research is a bottleneck problem in biochemistry and molecular biology (Dill KA, et al. Curr. Opin. Struct. Biol. 2007, 17: 342-346). The content includes the problem of protein structure prediction that explores the determination of amino acid sequence to native conformation (Dunbrack RL Jr. W. A. ​​et al. Annu. Rev. Biophys. Biomol. Struct. 2000, 29:327-359).

[0003] In experiments, in vitro denaturation and renaturation experiments are commonly used. That is to use the denaturant guanidine hydrochloride or urea to destroy the hydrogen bonds in the protein structure, so that it cannot maintain the natural three-dimensional conformation and become a relatively loose random coil. Then suddenly dilute the denaturant by stop-flow me...