Method for labelling human hepatic tissue capillary bile duct with CD25/IL-2Ra antibody

A technology of capillary bile duct and CD25, which is applied in fluorescence/phosphorescence, material analysis through observation of the influence of chemical indicators, material inspection products, etc., to achieve excellent expression effect, correct positioning, and excellent color rendering effect
CN101021528AInactive Publication Date: 2007-08-22无锡市传染病医院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
无锡市传染病医院
Publication Date
2007-08-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method that using CD25 / IL-2Ra antibody marks capillary bile duct of human liver organize, it belongs to histiocyte coloration expression technology field. This invention uses human liver puncture biopsy as specimen, the specimen is repaired by antigen, uses CD25 / IL-2Ra as the first antibody, color and flaking by immunity grouping or immunity reflected color, ensure its specificity by interdiction test, and ensure the marked capillary bile duct of human liver organize. Using two CD25 / IL-2Ra in different clone number as the first antibody, immunity grouping liver capillary bile duct is brow yellow bar structure, the expression effect are good, or the interdiction test ensure that the liver capillary bile duct expresses the specificity of CD25 / IL-2Ra by the result that the immunity reflected color liver capillary bile duct has green fluorescence. We set antitheses between masculine antitheses(tonsils) and CD4,CD8, the result indicates that the color of immunity grouping is in focus, the orientation is correct, the same organization use CD25 / IL-2Ra and CEA as antitheses separately, it proves that the CD25 / IL-2Ra is more specific than CEA, its color effect is well than CEA, it is benefit for the live capillary bile duct distributing and morphologic observation.
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Description

technical field

[0001] a CD 25 The invention relates to a method for labeling human liver tissue capillary bile duct with / IL-2Ra antibody, which belongs to the technical field of tissue cell staining and expression. Background technique

[0002] In recent years, with the in-depth understanding of liver diseases, liver biopsy has become a routine diagnostic method. Advances in puncture instruments and techniques have made liver puncture safer and more satisfying. In daily work, the liver biopsy can often reach more than 2cm, which makes the pathology of liver biopsy more representative and convincing, and further promotes the progress of diagnosis and treatment of liver diseases.

[0003] Routine liver histopathology includes HE (hematoxylin-eosin staining), reticulum staining, Masson staining, immunohistochemistry, special staining, in situ hybridization, etc. Observation of the ultrastructure of liver cells often requires electron microscopy, such as the observation of ...

Claims

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