Method for labelling human hepatic tissue capillary bile duct with CD25/IL-2Ra antibody

A technology of capillary bile duct and CD25, which is applied in fluorescence/phosphorescence, material analysis through observation of the influence of chemical indicators, material inspection products, etc., to achieve excellent expression effect, correct positioning, and excellent color rendering effect

Inactive Publication Date: 2007-08-22
无锡市传染病医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

cd 25 / IL-2Ra is expressed in the hepatic capillaries, but there is no literature report

Method used

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  • Method for labelling human hepatic tissue capillary bile duct with CD25/IL-2Ra antibody
  • Method for labelling human hepatic tissue capillary bile duct with CD25/IL-2Ra antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Immunohistochemistry: Capillary CD 25 / IL-2Ra labeling is brownish-yellow cord-like structure, and CD is performed on the same tissue at the same time 4 、CD 8 For lymphocyte control, tonsil tissue was used as positive control. The selected cases include chronic hepatitis B, PBC (primary biliary cirrhosis), chronic rejection after two years of liver transplantation, normal liver donors, drug-induced hepatitis and other tissues. The above organizations are composed of two CDs with different clone numbers 25 / IL-2Ra was used as the primary antibody, and the expression effects were excellent.

Embodiment 2

[0032] Using internationally recognized CEA (polyclonal antibody) and CD 25 / IL-2Ra control, the same liver tissue CD 25 The color development effect of / IL-2Ra is better than that of CEA.

Embodiment 3

[0034] Fluorescent immunohistochemical staining: Liver tissue and normal human blood from cell smears.

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PUM

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Abstract

A method that using CD25/IL-2Ra antibody marks capillary bile duct of human liver organize, it belongs to histiocyte coloration expression technology field. This invention uses human liver puncture biopsy as specimen, the specimen is repaired by antigen, uses CD25/IL-2Ra as the first antibody, color and flaking by immunity grouping or immunity reflected color, ensure its specificity by interdiction test, and ensure the marked capillary bile duct of human liver organize. Using two CD25/IL-2Ra in different clone number as the first antibody, immunity grouping liver capillary bile duct is brow yellow bar structure, the expression effect are good, or the interdiction test ensure that the liver capillary bile duct expresses the specificity of CD25/IL-2Ra by the result that the immunity reflected color liver capillary bile duct has green fluorescence. We set antitheses between masculine antitheses(tonsils) and CD4,CD8, the result indicates that the color of immunity grouping is in focus, the orientation is correct, the same organization use CD25/IL-2Ra and CEA as antitheses separately, it proves that the CD25/IL-2Ra is more specific than CEA, its color effect is well than CEA, it is benefit for the live capillary bile duct distributing and morphologic observation.

Description

technical field [0001] a CD 25 The invention relates to a method for labeling human liver tissue capillary bile duct with / IL-2Ra antibody, which belongs to the technical field of tissue cell staining and expression. Background technique [0002] In recent years, with the in-depth understanding of liver diseases, liver biopsy has become a routine diagnostic method. Advances in puncture instruments and techniques have made liver puncture safer and more satisfying. In daily work, the liver biopsy can often reach more than 2cm, which makes the pathology of liver biopsy more representative and convincing, and further promotes the progress of diagnosis and treatment of liver diseases. [0003] Routine liver histopathology includes HE (hematoxylin-eosin staining), reticulum staining, Masson staining, immunohistochemistry, special staining, in situ hybridization, etc. Observation of the ultrastructure of liver cells often requires electron microscopy, such as the observation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/78G01N21/64
Inventor 陆忠华印永强黄利华居朝霞胡锡琪邢益平蒋祥虎华惠琦
Owner 无锡市传染病医院
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