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Quantitative lateral flow system and assay

A lateral flow, test strip technology used in qualitative and quantitative assays and systems to solve problems such as lack of

Active Publication Date: 2007-09-12
RELIA BIOTECH SHENZHEN LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] To date, there has been a lack of rapid, efficient, and efficient quantitative lateral flow assays and systems that can be used to measure analytes in biological samples, such as blood samples, in a point-of-care environment, or that can be used to measure Lateral flow assays and systems that result in analytes in small sample sizes, or that can be used to measure analytes independently of the amount, or that solve other problems in prior art lateral flow assays and systems

Method used

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  • Quantitative lateral flow system and assay

Examples

Experimental program
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Effect test

example 1

[0200] Example 1: Filtration performance of membranes in the absence of lectins

[0201] The test strip shown in Figure 2 uses different membranes as sample filters. All tested filter membranes were purchased from Ahlstrom Filtration, USA and included: cellulose absorbent grade 111, glass fibers grade #141 and #142, Cytosep grades 1660, 1661, 1662 and 1663. As shown in Figure 1, a sample containing whole blood is applied to window 1. The flow velocity of plasma on the nitrocellulose chromatography strip can be observed and recorded. The results are listed in Table 2.

[0202]

example 2

[0203] Example 2: Blood filtration capacity of membranes pretreated with anti-human erythrocyte antibody

[0204] Add 1 gram of TWEEN20 (TWEEN20) to 9ml of deionized water, mix the solution, store it at room temperature for about a week, and prepare a 10% solution of TWEEN20 (TWEEN20).

[0205] Add 9.0825 g of Tris(hydroxymethyl)aminomethane (Trizma Base) (final concentration is 6.055 g / L), 1.7625 mL of HCL (final concentration is 1.7625 mL / L) and 1.8 g Disodium ethylenediaminetetraacetic acid (EDTA.Na2) (final concentration: 1.2 g / L) can be formulated into rabbit anti-human erythrocyte antibody solution. The mixture was stirred slowly for about an hour until all added chemicals were completely dissolved. The prepared solution was stored at room temperature for 4 hours, or overnight at 4°C. Hydrochloric acid was added to the solution to adjust the pH of the solution to pH 8.5±0.1. Rabbit anti-human red blood cell antibody (anti-hRBC) was added to the solution to a final con...

example 3

[0210] Example 3: Comparison between using whole blood and using plasma

[0211] Prepare the test strip as in Example 2, add the whole blood or plasma sample to the sample filter of window 1, and compare the results, as shown in Table 4.

[0212]

[0213] Experimental results show that both whole blood and plasma samples can be used with the test strip of the present invention, and both can obtain substantially consistent quantitative results.

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Abstract

The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, such as samples containing cells and fluid, where the assay is volume independent, and the sample size is less than about 100 l, where the test strip includes a first membrane such as a sample filter, that is in capillary contact with an optional second membrane, such as a fluid collector, the second membrane, if present is in capillary contact with an optional third membrane, such as a conjugate pad containing a mobilizable detectable agent, or with a fourth membrane, which is a chromatographic strip, which optionally contains a mobilizable detectable agent, all such membranes being in fluid contact with a fifth membrane, such as a buffer pad, a sixth membrane, such as an absorbent pad, optionally a seventh membrane, which is also an absorbent pad, a capture band for capturing the analyte and at least one control band, or alternatively, the chromatographic strip contains the mobilizable detectable agent in place of a conjugate pad, where the test strip is configured to support removal of red blood cells from the sample and to allow uni-directional or bi-directional fluid flow of fluid from the sample filter to the capture band to be retained therein and detected thereon.The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, where the assay is volume independent, and the sample size is less than about 100 l, where the test strip includes a first membrane (12, 18) that is in capillary contact with an optional second membrane, the second membrane (9), if present is in capillary contact with an optional third membrane (13), containing a mobilizable detectable agent, or with a fourth membrane (9), all such membranes being in fluid contact with a fifth membrane (14), a sixth membrane (15).

Description

[0001] Cross References to Related Applications [0002] This application hereby claims U.S. Provisional Patent Application No. 60 / 576,327, filed June 2, 2004, by Zhou et al., entitled "Quantitative Lateral Flow System and Assay," and the same title, filed July 29, 2004 by Zhou et al. Priority to US Provisional Patent Application No. 60 / 592,202 for "Quantitative Lateral Flow System and Assay." The disclosures of these provisional applications are hereby incorporated by reference in their entirety. technical field [0003] The present application generally relates to qualitative and quantitative assays and systems for detecting the presence of at least one analyte in biological samples, particularly samples comprising whole blood, red blood cells, white blood cells, or other cell types, and determining or quantifying the amount of the at least one analyte present. Background technique [0004] Lateral flow assays designed to detect the presence and quantity of analytes in b...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 周思亮W·J·若特刘宁
Owner RELIA BIOTECH SHENZHEN LTD
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