Technique of extracting polysaccharide in rosa laevigata michx

An extraction process, the technology of Rosa roxburghii, applied in the field of extraction process of natural plants, can solve the problems of low recovery rate, low purity, complicated operation, etc., and achieve the effect of high recovery rate, high purity, and simple operation

Inactive Publication Date: 2007-09-19
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular weight of Fructus Rosa Fructus polysaccharide fractionated by methanol or ethanol solution has a wide range of molecular weight, the purity is lower than 45%, and the recovery rate is less than 80%; Less than 75%, and the opera

Method used

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  • Technique of extracting polysaccharide in rosa laevigata michx
  • Technique of extracting polysaccharide in rosa laevigata michx
  • Technique of extracting polysaccharide in rosa laevigata michx

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Rough extraction process: wash 1 kg of Rosa roxa raw material, dry at 50°C for 48 hours, crush, pass through a 40-mesh sieve, add 2L of petroleum ether, reflux at 60-65°C for 2 hours, cool to room temperature, add a weight concentration of 80 % ethanol 1.5L, stand for 3h, evaporate the solvent, add the product to 10L of water, reflux at 90-95°C for 4h, cool to room temperature, filter, and concentrate the filtrate to 1L at a temperature of 60°C and a pressure of -1.9Mpa .

[0021] Deproteinization process: deproteinize 1L of concentrated solution by Sevage method 6 times, add 3.5L of ethanol to the separated solution for alcohol analysis, add 1L of ether and 1L of acetone to wash the precipitate, and vacuum freeze-dry the precipitate to obtain mixed polysaccharides (RPs) 165g.

[0022] Classification process: Diethylaminoethyl cellulose is used as filler, the mass ratio of diethylaminoethyl cellulose to mixed polysaccharides (RPs) is 10:1, and the flow rate is 1.5ml / mi...

Embodiment 2

[0026] Rough extraction process: wash 1 kg of Rosa roxa raw material, dry at 50°C for 48 hours, crush, pass through a 40-mesh sieve, add 2L of petroleum ether, reflux at 60-65°C for 2 hours, cool to room temperature, add a weight concentration of 80 % ethanol 1.5L, stand for 3h, evaporate the solvent, add the product to 10L of water, reflux at 90-95°C for 4h, cool to room temperature, filter, and concentrate the filtrate to 1L at a temperature of 60°C and a pressure of -1.9Mpa .

[0027] Deproteinization process: deproteinize 1L of concentrated solution by Sevage method 7 times, add 3.5L of ethanol to the separated solution for alcohol analysis, add 1L of ether and 1L of acetone to wash the precipitate, and vacuum freeze-dry the precipitate to obtain mixed polysaccharides (RPs) 159g.

[0028] Classification process: Diethylaminoethyl cellulose is used as a filler, the mass ratio of diethylaminoethyl cellulose to mixed polysaccharides (RPs) is 12:1, and the flow rate is 1.2ml / ...

Embodiment 3

[0031] Rough extraction process: wash 1 kg of Rosa roxa raw material, dry at 50°C for 48 hours, crush, pass through a 60-mesh sieve, add 2L of petroleum ether, reflux at 60-65°C for 2 hours, cool to room temperature, add a weight concentration of 80 % ethanol 1.5L, stand for 3h, evaporate the solvent, add the product to 10L of water, add 200mg of proteinase K, incubate at 37°C for 2h, heat up to 90-95°C, reflux for 4h, cool to room temperature, filter, and the filtrate is heated at 50°C °C, concentrated to 1L under the pressure of -1.9Mpa.

[0032] Deproteinization process: deproteinize 1L of the concentrated solution twice by Sevage method, add 3.5L of ethanol to the separated solution for alcohol analysis, add 1L of ether and 1L of acetone to wash the precipitate, and vacuum freeze-dry the precipitate to obtain mixed polysaccharides (RPs) 183g.

[0033] Classification process: Diethylaminoethyl cellulose is used as filler, the mass ratio of diethylaminoethyl cellulose to mi...

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Abstract

The invention discloses an extraction process of rosa laevigata michx, comprising a crude extracting step, a deproteinizing step and a classifying step. The classifying step is in that: an ion exchanger as a filler which has weight ratio of 10-25:1 with samples prepared in the deproteinizing step and a neutral salt solution having ionic strength of 0-5 mol/l are gradient eluted, picked up, decompress concentrated, dialyzed, and freeze dried. Compared with the existing technology, the invention has advantages of high purity, high rate of recovery and simple operation. The rosa laevigata michx with average molecular weight of 18000~20000 prepared by the invention has remarkable lowering blood lipid effects.

Description

Technical field: [0001] The invention belongs to the technical field of extraction technology of natural plants, in particular to the technical field of extraction technology of Fructus Rosa Rosa polysaccharide. Background technique: [0002] As a traditional Chinese medicinal material, Fructus Rosa Fructus has a wide range of uses. Fructus Fructus Fructus contains proteins, polysaccharides, polyphenols, malic acid, citric acid, tannins and saponins. It has been found through research and proved by experiments: Fructus Fructus Fructus Polysaccharides have anti-oxidation, hypolipidemic, immune, and antibacterial effects, so the research on the extraction technology of Fructus Rosa Fructus polysaccharides is becoming more and more urgent, but there are very few reports on the extraction methods of Fructus Rosa Fructus polysaccharides at home and abroad. It is known that the extraction process of the polysaccharides of Rosa chinensis includes a crude extraction process, a depro...

Claims

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Application Information

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IPC IPC(8): A61K36/738A61K31/715A61P3/06C08B37/00C12P19/04A61K131/00
Inventor 聂刘旺张庭廷陈传平何梅吴安平
Owner ANHUI NORMAL UNIV
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