Cell freezing apparatus

Abstract

The invention relates to a cell freezing device. It comprises temperature control pipe and temperature checking device installed on sample shelf on low- temperature box. It is characterized in that: temperature control pipe is composed of copper pipe, insulating layer and electrical heating wire; copper pipe is installed on sample pipe, the out of copper pipe is covered with insulating layer, the insulating layer is wound with electrical heating wire; temperature control device comprises temperature data gathering device and temperature control device, temperature data gathering device includes temperature control pipe, thermal couple; thermal couple is inserted into temperature control pipe, the thermal electic signal of thermal couple controls electrical heating wire after passing through temperature control pipe. The device can solve problems of biological cell temperature reduction and temperature recover control, and is characterized by high sample temperature evenness, effective temperature recover device, fast and in time response, low production cost, convenient operation, and wide application for programme temperature reduction and recover for various biological materials low- temperature restorage.

Description

technical field [0001] The invention relates to a temperature control device for biological materials, in particular to a device for freezing and cryopreserving biological cells using programmed cooling and rewarming. Background technique [0002] In industries such as medicine, biology, agriculture, animal husbandry and fishery, some biological materials need to be stored at low temperature. Such as cryopreservation of biological materials such as cornea, blood, bone marrow, semen, embryos, etc. With the development of transplantation research, the demand for various cells is increasing, and effective long-term storage of cells is urgently needed to promote the role of transplantation in the clinic. Low temperature preservation is an important method for preserving various cells at present. In order to meet the requirements of low temperature preservation for the cooling process, there are various cooling methods. At present, there is a two-step cooling method using a ref...

AI Technical Summary

Problems solved by technology

The structure of the liquid nitrogen jet cooling instrument is complex and expensive, and its low-temperature solenoid valve is prone to frost under low temperature conditions, causing the valve to be closed tightly or unable to operate; and the liquid nitrogen consumption is relatively large. Supplementing the liquid nitrogen container with liquid nitrogen will interfere or destroy the cooling procedure

Although the lifting type cooling instrument has a simple structure, since the cooling and rewarming are carried out by using the temperature gradient in the gas phase region in the neck tube of the liquid nitrogen container, it is difficult to control the uniformity of the temperature in the cooling test of placing a slender sample tube

None of the above cooling methods have effective rewarming control.

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