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Bioassay equipment and bioassay method

A bioassay and equipment technology, applied in biochemical equipment and methods, biological testing, biological material pretreatment, etc., can solve the problems of precipitation, fixation, concentration change, etc., to achieve high-precision bioassay, easy adjustment, and cost reduction. Effect

Inactive Publication Date: 2007-12-26
SONY CORP
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  • Claims
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AI Technical Summary

Problems solved by technology

This volume change of the medium causes the following problems: the concentration of the substance m (probe substance, target substance, etc.) in the reaction region R is changed, or the substance m in the reaction region R is precipitated, fixed
As a result, the concentration of the substance varies between the individual reaction regions R, adversely affecting the detection accuracy

Method used

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  • Bioassay equipment and bioassay method
  • Bioassay equipment and bioassay method
  • Bioassay equipment and bioassay method

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Embodiment Construction

[0043] Hereinafter, preferred embodiments of the present invention are described with reference to the accompanying drawings. It should be noted that the embodiments shown in the drawings merely illustrate representative examples of the apparatus and method of the present invention, and the scope of the present invention is not limited to these examples.

[0044]First, Figure 1 is a cross-sectional view showing the configuration of components in a substrate with a reaction zone suitable for use in the bioassay device and method of the present invention.

[0045] The substrate 1 is a plate for biological measurement, and is composed of a lower substrate 11 and an upper substrate (hereinafter referred to as "cover") 12 superimposed on the lower substrate by bonding or the like. The lower substrate 11 has a layered structure, wherein the lower substrate 111 , the transparent electrode layer 112 , the fixed layer 113 and the reaction region forming layer 114 with the reaction regi...

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Abstract

Bioassay equipment which prevents a variation in concentration of a medium or deposition / bonding of a substance in the medium to be caused by drying of the medium being stored or held in a reaction region providing an inter-substance interaction field. The bioassay equipment (2) comprises at least a means for supplying a medium containing a substance pertaining to the interaction to the reaction region R providing the field of inter-substance interaction such as hybridization, and a means for supplying required water automatically. The bioassay equipment (2) may comprises a means for automatically detecting the volume of the medium held in the reaction region R.

Description

technical field [0001] The present invention relates to bioassay devices and bioassay methods. More specifically, the present invention relates to bioassay devices and bioassay methods, which are designed to prevent changes in the concentration of media residing or held in the reaction zone, which provides for the interaction between substances, due to drying. place. Background technique [0002] In recent years, integrated bioassay plates obtained by microarranging the required DNA by microarray technology, commonly referred to as "DNA chips" or "DNA microarrays" (hereinafter collectively referred to as "DNA chips"), have become more and more popular. It is widely used in gene mutation analysis, SNP (single nucleotide polymorphism), gene expression frequency analysis, etc., and has begun to be widely used in drug development, clinical diagnosis, pharmacogenomics (pharmacogenomics), evolutionary research, forensic science and other field. The "DNA chip" is characterized i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C12M1/00G01N37/00C12N15/09
CPCB01L3/5085B01L2300/0806G01N35/00069B01L2200/142B01J2219/00378B01J2219/00722B01J2219/00536B01L2300/10C12M1/00C12N15/09G01N37/00C12M1/34
Inventor 伊藤达巳武田实古木基裕山根良一中岛敏弘
Owner SONY CORP
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