Extractive of bdella and/or lumbricus with the molecular weight below 5800 dalton

A technology of extract and molecular weight cut-off, which is applied in the field of medicine for the treatment of thrombotic diseases, preparation of prevention, extracts with a molecular weight below 5800 Daltons of leeches and/or earthworms, to achieve the effect of improving safety

Inactive Publication Date: 2008-01-02
李振国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

subject to strict limits

Method used

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  • Extractive of bdella and/or lumbricus with the molecular weight below 5800 dalton
  • Extractive of bdella and/or lumbricus with the molecular weight below 5800 dalton
  • Extractive of bdella and/or lumbricus with the molecular weight below 5800 dalton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Take the raw material of leech medicine (dry or fresh), soak it with normal saline or water for injection, make its surface fully spread out, then wash it repeatedly with normal saline or water for injection, and place it in 2-4 times the volume of normal saline or water for injection. Soak for 24 hours at a low temperature of 0-4 degrees Celsius, filter, and store the filtrate and medicinal residues separately for later use.

[0057] Use a tissue masher to mash the dregs, then use a colloid mill to mix and homogenize the above filtrate, grind it into a homogenate with a diameter of less than 0.5 microns, freeze the homogenate at minus 15 degrees Celsius for 15-30 hours, take it out, and store it at a low temperature Thaw at 0-4 degrees Celsius, and then freeze and thaw repeatedly at least 2 times according to the above conditions.

[0058] The above-mentioned freeze-thawed liquid was placed in a centrifuge for centrifugation to obtain a supernatant, which was subjected...

Embodiment 2

[0062] Take earthworm medicinal material raw material (dried product or fresh product). Carry out extraction and separation according to the same method as described in Example 1, and make the earthworm extract. After detecting the content of substances with high molecular weight (molecular weight greater than 5800 Daltons), no substances with molecular weights greater than 5800 Daltons were found in the extract. Therefore, the molecular weight of the obtained earthworm extract is below 5800 Daltons.

[0063] If necessary, the extract obtained above can be dehydrated and dried into a solid.

Embodiment 3

[0065] Get equal amount of leech (dry product), earthworm (dry product), extract and separate according to the same method described in Example 1, respectively make leech extract and earthworm extract, and prepare two kinds of extracts After mixing evenly, the leech earthworm extract (hereinafter referred to as the extract of the present invention) was obtained. After the detection of the content of substances with high molecular weight (molecular weight greater than 5800 Daltons), no substances with molecular weight greater than 5800 Daltons were found in the extract. The results are shown in Figures 1 and 2.

[0066] The peak data in Figure 1 and Figure 2 are shown in Table 1 and Table 2, respectively.

[0067] Table 1 Detection results of human insulin

[0068] peak number

keep time

(min)

Types of

Width (min)

Area (mAU)

Area percentage

(%)

1

12.027

BB

0.2851

3618.27271

100

Tota...

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Abstract

The invention provides a kind of extract with its molecular weight being lower than 5800 dalton from hirudo and / or lumbricus, the preparation method, medicine compound and its application. The mentioned extract is better than current hirudo and / or lumbricus extract in terms of visible foreign matter and insoluble micro-particle and product safety. The product in this invention can be used to produce medicine for preventing and treating thrombus relevant diseases.

Description

technical field [0001] The present invention relates to an extract of leeches and / or earthworms, specifically, an extract of leeches and / or earthworms with a molecular weight below 5800 Daltons, as well as its pharmaceutical composition, preparation method and use, for the preparation of Drugs for the prevention and treatment of thrombotic diseases. Background technique [0002] As a classic drug for breaking blood and removing stasis, leeches are included in the Chinese Pharmacopoeia. There are three kinds of leech medicinal materials recorded in the Chinese Pharmacopoeia, among which the main species used as medicine is the leech (Whitmania pigra Whitman). The main components of leeches are proteins, polypeptides, amino acids, nucleic acids, enzymes, sugars and trace elements. There have been many reports on the qualitative and quantitative studies of proteins, amino acids, sugars and trace elements in leeches, and the main methods are thin-layer chromatography, high per...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K35/62A61K38/02A61K9/00C07K1/34A61P3/06A61P7/02A61P9/10A61P1/16A61P13/12A61P25/02A61P27/00
CPCA61K35/64A61K35/62A61P1/16A61P1/18A61P3/06A61P3/10A61P7/02A61P9/00A61P9/08A61P9/10A61P11/00A61P13/12A61P17/02A61P25/00A61P27/02A61P27/16A61K35/56
Inventor 李振国
Owner 李振国
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