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Method for purifying 5-inosinic acid zymotic fluid by crystallization

A technology for fermentation broth and inosinic acid, applied in the field of purification of disodium 5'-inosinic acid, can solve the problems of multiple waste water/waste liquid, complicated management and operation, etc.

Inactive Publication Date: 2008-01-23
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the disadvantages of producing more waste water / waste liquid, complicated management and operation, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Utilize the method of crystallization (1 to 3 times) through a hydrophilic organic solvent

[0031] In this example, 6,000 ml of fermented liquid containing IMP was heated to 40°C, and then passed through a membrane filter (Ceramic Filter Pilot: a pore size of 0.1μm, area 1.0m 2 ) to filter and separate into bacterial precipitate and filtrate. Then, the above-mentioned filtrate was dried under reduced pressure at a vacuum degree of 680 mmHg and a container temperature of 55° C. to obtain 1,950 ml of a concentrated solution with an IMP content of 25%. After adjusting the pH of the concentrated solution to 7.5, the Inject it into the crystallization tube. Then, 3,120ml of 95 volume % methanol aqueous solution was injected for 6 hours. When starting to inject methanol, the temperature of the crystallization tube was 70° C., and when the injection of methanol was completed, the temperature of the crystallization tube was 40° C. After the injection and addition...

Embodiment 2

[0037] Example 2: Using the method of crystallization (1-2 times)+concentration / crystallization (3 times) through a hydrophilic organic solvent

[0038] In this example, 3,900 ml of fermented liquid containing IMP was heated to 50°C, and then passed through a membrane filter (Ceramic Filter Pilot: a pore size of 0.1μm, area 1.0m 2 ) to filter, and separate the bacterial precipitate from the filtrate. Then, the above-mentioned filtrate was dried under reduced pressure at a vacuum degree of 680 mmHg and a container temperature of 55° C. to obtain 2,430 ml of a concentrated solution with an IMP content of 20%. After adjusting the pH of the concentrated solution to 8.0, the It is injected into the crystallization tube. When the injection of methanol started, the temperature of the crystallization tube was 65° C., and when the injection and addition of methanol was completed, the temperature of the crystallization tube was 30° C. After the injection and addition of methanol was ...

Embodiment 3

[0044] Example 3: Method of crystallization (1 time) + concentration / crystallization (2 to 3 times) by using a hydrophilic organic solvent

[0045] In this example, 4,600 ml of fermented liquid containing IMP was heated to 50°C, and then passed through a membrane filter (Ceramic Filter Pilot: pore size of 0.1μm, area 1.0m 2 ) to filter, and separate the bacterial precipitate from the filtrate. Then, the above-mentioned filtrate was dried under reduced pressure at a vacuum degree of 680 mmHg and a container temperature of 55° C. to obtain 1,010 ml of a concentrated solution with an IMP content of 30%. After adjusting the pH of the concentrated solution to 8.5, the It is injected into the crystallization tube. Then, 1,515 ml of 95 volume % methanol aqueous solution was injected for 4 hours. When the injection of methanol was started, the temperature of the crystallization tube was 75° C., and when the injection of methanol was completed, the temperature of the crystallization ...

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PUM

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Abstract

The invention relates to a method for purifying the 5'-IMP2Na from a 5'-IMP fermenting liquor generated by the microbial fermentation. The invention improves the prior purification procedure, substitutes the prior procedure of an anion exchange resin vessel so as to reduce the yield of the waste water / waste liquid and the usage of the chemical medicine of the acid and alkali and the like in a large scale.

Description

technical field [0001] The invention relates to a purification method of disodium 5'-inosinate (IMP2Na). Specifically, it relates to a method for purifying 5'-inosinic acid disodium salt from 5'-inosinic acid (IMP) fermentation broth by crystallization process. Background technique [0002] Purine derivative nucleotides such as IMP are often used as raw materials for the prevention and treatment of metabolic disorders, growth promoters, anticancer agents, etc. in medicine, and are often used as important substances in food additives such as seasonings in food . In particular, IMP, as a taste component of food, is a substance naturally present in foods such as meat and fish. It can be extracted by fish meat extraction, RNase decomposition, microbial fermentation, and a combination of fermentation and synthesis. , RNA chemical decomposition and synthesis and other methods of production. However, no matter by which method it is produced, its products will contain ingredients...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/32C07H1/06
CPCC07B2200/13C12P19/32
Inventor 李允宰朴南燮韩胜宇李恩硕尹太相俞载宪洪淳源金庾赆
Owner CJ CHEILJEDANG CORP
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