Unlock instant, AI-driven research and patent intelligence for your innovation.

Furbenicillin sodium and its formulation content high-performance liquid chromatogram determination method

A technology of high-performance liquid chromatography and furbucillin sodium, which is applied in the field of high-performance liquid chromatography determination of the content of furbuxilin sodium and its preparations, can solve the problems of high-performance liquid chromatography, and achieve strong practicability and Operability and the effect of reducing the inspection workload

Inactive Publication Date: 2008-02-20
张菁
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the raw materials and their preparations are included in the first volume of the Ministry of Health's drug standard antibiotic drugs, the 1989 edition, and the standard number is WS1-C2-0021-89. Chromatographic content determination and related substance inspection methods have not yet been reported to apply for administrative protection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Furbenicillin sodium and its formulation content high-performance liquid chromatogram determination method
  • Furbenicillin sodium and its formulation content high-performance liquid chromatogram determination method
  • Furbenicillin sodium and its formulation content high-performance liquid chromatogram determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The high-performance liquid chromatography assay method of furubusillin sodium and its preparation content, its chemical molecular formula is

[0016]

[0017] The test method is as follows:

[0018] (1) High performance liquid chromatography is adopted, chromatographic conditions: octadecylsilane bonded silica gel is used as filler; mobile phase phosphate buffer: methanol 50:50, phosphate buffer is adjusted to pH 3.5 with phosphoric acid 0.1mol / L potassium dihydrogen phosphate solution; flow rate 0.9ml per minute; detection wavelength 220nm; column temperature: 10°C room temperature;

[0019] (2) Preparation of furbucillin reference substance solution: accurately get 25 mg of furbuscillin reference substance and put it in a 50ml volumetric flask, dissolve it with water and dilute to the mark;

[0020] (3) Preparation of the test solution: accurately weigh 25 mg of furbuxilin and put it in a 50 ml volumetric flask, dissolve it with water and dilute to the mark;

[...

Embodiment 2

[0023] The difference from Example 1 is that in the chromatographic conditions of the first step, the flow rate is 1.2 ml per minute; the detection wavelength is 223 nm; the column temperature is 20° C. at room temperature.

Embodiment 3

[0025] The difference from Example 1 is that the flow rate in the first step of chromatographic conditions is 1.5ml per minute; the detection wavelength is 225nm; column temperature: 30°C room temperature.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The utility model discloses a high performance liquid chromatography method for fubuxilin sodium and the preparation content, comprising following steps: firstly, control substance solution and test solution are prepared, then 10 Mu L of the test solution and the control substance solution are injected into a color spectrometer, the chromatography is recorded, and the content of the obtained fubuxilin sodium and the injection of fubuxilin sodium with the peak area is calculated according to the external standard method. The method can not only effectively and accurately test the content of the fubuxilin sodium and the preparation content, and can greatly reduce the testing workload of the relevant enterprises and supervision testing departments; therefore, the method is very practicable and workable in the production of the drug and the preparation and the control of quality.

Description

technical field [0001] The invention relates to a method for determining the content of antibiotic drugs, in particular to a high-performance liquid chromatography method for determining the content of furubusillin sodium and its preparations. Background technique [0002] Furbusillin sodium is a urea-based derivative of aminopenicillin. It is a broad-spectrum semi-synthetic penicillin with strong antibacterial activity. Bacteria and other Gram-negative bacteria have good antibacterial effect, and it is mainly used clinically for infections caused by Pseudomonas aeruginosa. At present, the raw materials and their preparations are included in the first volume of the Ministry of Health’s drug standard antibiotic drugs, the 1989 edition, and the standard number is WS1-C2-0021-89. Chromatographic content determination and related substance inspection methods have not been reported to apply for administrative protection. Contents of the invention [0003] The technical proble...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张菁王金龙姜建国
Owner 张菁