Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte

Abstract

The invention discloses a continuous filling culture gas-lift biological reactor system of medicinal plant tissue cell in the biological technical domain, which comprises the following parts: liquid storage bottle, reactor host, gravity sediment device, waste liquor bottle, tissue / organ continuous acquiring device, duplex sampling bottle, liquid pump, oxygen probe, pH value probe, on-line monitor system and computer, wherein the liquid storage bottle, liquid pump, reactor host, gravity sediment device and waste liquor bottle connect through silica gel pipe sequently; the oxygen probe and pH value connect computer through on-line monitor system. The invention can be applied for culturing kinds of different tissue, cell and organ to save cost, which avoids the damage of plant tissue cell for medicinal plant tissue, cell and organic suspension culture.

Description

technical field [0001] The invention relates to a system in the field of biotechnology, in particular to an airlift bioreactor system for continuous perfusion culture of medicinal plant tissue cells. Background technique [0002] my country is one of the countries with the richest medicinal plant resources in the world. As the earliest natural resources developed, medicinal plant resources are currently facing two crises: one is that the reserves and output of wild plant resources are generally declining; the other is that The demand for medicinal plants continues to increase, resulting in excessive mining of medicinal plant resources. On the one hand, the traditional field cultivation of medicinal plants is affected by "time and place", resulting in large fluctuations in yield and quality; on the other hand, it inevitably brings about problems such as pesticide residue pollution. Tissue and cell bioreactor culture has the characteristics of rapid growth and easy control, wh...

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Problems solved by technology

But the defect and deficiency of described reactor are: the baffle plate placed in the downcomer and the pipe wall are not easy to seal; the riser and downcomer of the reactor are all cylinders in addition, which is not conducive to plant tissues, especially roots and plants. The stretching of the reactor; the reactor gas nozzle is located at the upper part of the bottom of the riser, and the place where the riser and the downcomer are connected is easy to accumulate cells

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