Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte

A technology of bioreactor and tissue cells, which is applied in the field of biotechnology systems, can solve the problems of unfavorable plant tissue roots and plant extension, difficult sealing, and easy accumulation of cells, so as to save costs, avoid damage, and save energy.

Inactive Publication Date: 2008-02-27
上海交通大学中原研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the defect and deficiency of described reactor are: the baffle plate placed in the downcomer and the pipe wall are not easy to seal; the riser and downcomer of the reactor are all cylinders in addition, which is not conducive to pla...

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  • Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte
  • Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte

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Embodiment Construction

[0024] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings: this embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following the described embodiment.

[0025] As shown in Figure 1, this embodiment includes: liquid storage bottle 1, reactor main body 4, gravity settling device 14, waste liquid bottle 15, tissue / organ continuous harvesting device 6, double sampling bottle 7, liquid pump 3, oxygen Probe 10, pH probe 11, online monitoring system 12, computer 13. The liquid storage bottle (1), the liquid pump 3, the reactor main body 4, the gravity sinking device 14, and the waste liquid bottle 15 are connected in sequence through a silicone tube. The oxygen probe 10 and the pH probe 11 are connected to a computer 13 thr...

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Abstract

The invention discloses a continuous filling culture gas-lift biological reactor system of medicinal plant tissue cell in the biological technical domain, which comprises the following parts: liquid storage bottle, reactor host, gravity sediment device, waste liquor bottle, tissue/organ continuous acquiring device, duplex sampling bottle, liquid pump, oxygen probe, pH value probe, on-line monitor system and computer, wherein the liquid storage bottle, liquid pump, reactor host, gravity sediment device and waste liquor bottle connect through silica gel pipe sequently; the oxygen probe and pH value connect computer through on-line monitor system. The invention can be applied for culturing kinds of different tissue, cell and organ to save cost, which avoids the damage of plant tissue cell for medicinal plant tissue, cell and organic suspension culture.

Description

technical field [0001] The invention relates to a system in the field of biotechnology, in particular to an airlift bioreactor system for continuous perfusion culture of medicinal plant tissue cells. Background technique [0002] my country is one of the countries with the richest medicinal plant resources in the world. As the earliest natural resources developed, medicinal plant resources are currently facing two crises: one is that the reserves and output of wild plant resources are generally declining; the other is that The demand for medicinal plants continues to increase, resulting in excessive mining of medicinal plant resources. On the one hand, the traditional field cultivation of medicinal plants is affected by "time and place", resulting in large fluctuations in yield and quality; on the other hand, it inevitably brings about problems such as pesticide residue pollution. Tissue and cell bioreactor culture has the characteristics of rapid growth and easy control, wh...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M29/08C12M29/10C12M41/26C12M41/34C12M41/48
Inventor 王贵荣齐念民张宝红闫继红廖凯
Owner 上海交通大学中原研究院
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