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Method for extracting melanin from black-bone chicken

A black-bone chicken and melanin technology, applied to chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of long extraction time of melanin, complex enzymatic hydrolysis conditions, cumbersome steps, etc., to reduce damage, operate Simple, cost-reducing effect

Inactive Publication Date: 2008-04-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages are high cost, complex enzymatic hydrolysis conditions, cumbersome steps, and long time to extract melanin

Method used

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  • Method for extracting melanin from black-bone chicken
  • Method for extracting melanin from black-bone chicken
  • Method for extracting melanin from black-bone chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method for extracting melanin from silky chickens comprises chopping the silky chickens, washing them with 6M hydrochloric acid solution for 10 minutes, and immersing them in liquid nitrogen for 1 minute. Crush the black-bone chicken immersed in liquid nitrogen into 80-mesh powder, transfer it to a Soxhlet extractor, and reflux with 6M hydrochloric acid solution for 2 hours to stop. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate was precipitated at 4°C, and the precipitate was first degreased and washed 4 times with acetone equal to the volume of the extract, then washed 4 times with distilled water equal to the volume of the extract, and dried.

Embodiment 2

[0020] The method for extracting melanin from black-bone chicken comprises chopping the black-bone chicken, washing with 1M hydrochloric acid solution for 30 minutes, and immersing in liquid nitrogen for 5 minutes. Crush the black-bone chicken immersed in liquid nitrogen into 100-mesh powder, transfer it to a Soxhlet extractor, and then stop it by refluxing with 1M hydrochloric acid solution for 3 hours. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate was precipitated at 5°C, and the precipitate was degreased and washed 3 times with acetone equal to the volume of the extract, then washed 3 times with distilled water equal to the volume of the extract, and dried.

Embodiment 3

[0022] The method for extracting melanin from silky chickens comprises chopping the silky chickens, washing them with 10M hydrochloric acid solution for 5 minutes, and immersing them in liquid nitrogen for 0.5 minutes. Crush the black-bone chicken immersed in liquid nitrogen into 60-mesh powder, transfer it to a Soxhlet extractor, and then stop it by refluxing with 10M hydrochloric acid solution for 1.5 hours. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate is precipitated at 0°C, and the precipitate is degreased and washed 3 times with acetone equal to the volume of the extract, then washed 3 times with distilled water equal to the volume of the extract, and dried.

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Abstract

The invention relates to a melanin extraction method from a black-bone silky fowl, which comprises the following steps: the black-bone fowl is cut up and washed by using 1 M to 10 M of hydrochloric acid solution for 5 minutes to 30 minutes and is immerged into liquid nitrogen for 0.5 minute to 5 minutes; after that, the black-bone fowl is grinded into 60 meshes to 100 meshes powder and transferred into a Soxhlet extractor to do thermal reflux for 1.5 hours to 3 hours by using 1 M to 10 M of hydrochloric acid; after the leaching liquor is cooled to 0 DEG C to 5 DEG C to be precipitated; acetone distilled water, which has the same volume with the leaching liquor, is respectively added into the precipitate for washing for 3 to 4 times, drying, then the melanin is made. Through the grinding process after the liquid nitrogen is frozen, the method of the invention which can fully and effectively damage the structure of melanophore in tissue under the precondition of no changing the physical chemistry properties of the melanin, is in favor of fast melanin release, shortens melanin extraction time and keeps nearly complete the melanin morphological structure of the black-bone fowl.

Description

technical field [0001] The invention relates to a method for extracting melanin, in particular to a method for extracting melanin from silky chicken. Background technique [0002] Silkie is a unique rare bird in my country, which has special nourishing and medicinal value. In "Compendium of Materia Medica", Li Shizhen said, "Silky chickens include white-haired black-boned chickens, black-haired black-boned chickens, spotted-haired black-boned chickens, black-boned chickens and white meat and black-boned chickens, but the tongue is black. Those who have it will have black bones and flesh, and it will be better when used as medicine." It can be seen that the amount of melanin in the meat and bones is an important criterion for ancient medicine to distinguish the medicinal effect of silky chicken. Modern medicine also believes that the medicinal value of silky chicken lies in the melanin substance in its body. Melanin is a complex and irregular polymer widely present in many...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 杨宁陈思睿蒋斌郑江霞
Owner CHINA AGRI UNIV
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