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Production of enzymes

A wild-type, hydrolytic enzyme technology, applied in the direction of enzymes, microorganisms, microorganisms, etc., can solve problems such as expensive

Inactive Publication Date: 2008-05-07
NOVOZYMES NORTH AMERICA INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Purified glucose and similar sugars are expensive

Method used

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  • Production of enzymes
  • Production of enzymes
  • Production of enzymes

Examples

Experimental program
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Embodiment 1

[0081] Liquefied and saccharified corn mash for cellulase production

[0082] Coarsely ground corn kernels were heated in water and treated with alpha-amylase (TERMAMYL TM SC from Novozymes) was processed to liquefy a thick mixture containing approximately 34% dry weight solids. By adding glucoamylase (SPIRIZYME TM FUEL from Novozymes) and incubated at 65°C for 48 hours, the mixture was saccharified. This mixture was centrifuged at approximately 4500 xg for 10 minutes to remove insoluble solids, filtered through Miracloth (Calbiochem), and analyzed by HPLC using an Aminex HPX87H column (BioRad) eluting with 5 mM sulfuric acid and detected with a refractive index detector. The resulting filtered corn mash supernatant was tested for sugar content. Standard solutions of glucose, cellobiose, xylose, and ethanol were used to proofread the quantification of detected sugars and ethanol. Typical concentrations of glucose in sugary corn mash supernatant syrup are about 300 g / lit...

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Abstract

The present invention relates to a process of producing desired enzyme(s) in a host cell, comprising cultivating said host cell capable of producing the desired enzyme(s) under conditions conducive for production of the desired enzyme(s) using a substrate for the host cell comprising liquefied and / or saccharified starch-containing plant material.

Description

Field of Invention [0001] The present invention relates to methods of producing the desired enzymes in host cells. Background technique [0002] Currently, microbial host cells are widely used to produce enzymes by fermentation. Enzymes are in great demand, especially for industrial use, eg, to convert starch-containing plant feedstocks into syrups and / or fermentation products, but are only available at relatively low prices. This makes the production cost of the enzyme an important factor for success in the market. The cost of the substrate can constitute as much as 40-50% of the total enzyme production cost. Substrates are available from commercial suppliers or prepared according to published compositions (eg, in catalogues of the American Type Culture Collection). The most commonly used carbon source substrates for enzyme production are purified glucose and similar sugars. Purified glucose and similar sugars are expensive. Therefore, there is a need to provide readil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N1/00C12N5/00C12N1/20C12P21/06C07H21/02
CPCC12P21/02
Inventor 马茨·P·T·史密斯吉勒玛·科沃德-凯利基思·C·麦克法兰德里克·S·阿克希尔姆
Owner NOVOZYMES NORTH AMERICA INC