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Method for synthesizing feruloylated oligosaccharides by biological catalysis

A feruloyl oligosaccharide and biocatalysis technology, applied in the direction of fermentation, etc., can solve the problems of difficult to obtain products, low extraction rate, difficult separation, etc., and achieve good utilization effect

Inactive Publication Date: 2008-06-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wheat bran contains many beneficial substances, such as feruloyl oligosaccharides, which can play the physiological role of ferulic acid and oligosaccharides, but the content of feruloyl oligosaccharides in wheat bran is very low, and the extraction rate is not high. Separation is more difficult, it is difficult to obtain purer products, which is not conducive to product development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The wheat bran is hydrolyzed to obtain different xylo-oligosaccharides, separated and purified, and then synthesized with ferulic acid under the catalysis of Novozymes lipase 435 in tert-butanol medium.

[0014] (1) Preparation of different oligosaccharides: wheat bran is processed to obtain insoluble dietary fiber, then hydrolyzed with xylanase to obtain a mixture of oligosaccharides, and then separated and purified to obtain xylo-oligosaccharides with different compositions, including xylose , Xylobiose, xylotriose, xylotetraose, xylo-oligosaccharides have arabinose attached to the side chain.

[0015] (2) Catalytic synthesis of ferulyl oligosaccharides: Weigh equimolar ferulic acid and oligosaccharides, add tert-butanol, and then add Novozymes Lipase 435 at 10% of the ferulic acid mass, 60℃ The reaction time is 10-12 hours, and the shaking rate of the shaker is 100 revolutions / min.

[0016] (3) Separation of the mixture after the reaction is completed: After the reaction...

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PUM

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Abstract

The invention relates to a method for biological catalysis and synthesis of ferulic acyl oligosaccharides and belongs to the ferulic acid derivative synthesis technical field. The invention takes ferulic acids and oligosaccharides separated from wheat bran as raw materials, and the ferulic acyl oligosaccharides are synthesized by catalysis of Novozymes lipases 435. Due to difference of the separated oligosaccharides, different ferulic acyl oligosaccharides can be obtained, thereby the invention is favorable for analysis of physicochemical property and other properties of the ferulic acyl oligosaccharides and utilizes the ferulic acyl oligosaccharides better.

Description

Technical field [0001] A method for biocatalytic synthesis of ferulyl oligosaccharides belongs to the technical field of the synthesis of ferulic acid derivatives. Background technique [0002] my country is a big wheat edible country, and the amount of wheat bran produced after wheat flouring is very large, about 20 million tons. Wheat bran contains many beneficial substances, such as ferulyl oligosaccharides, which can play the physiological role of ferulic acid and oligosaccharides. However, the content of ferulyl oligosaccharides in wheat bran is very low and the extraction rate is not high. Separation is more difficult, and it is difficult to obtain a purer product, which is not conducive to product development. [0003] Using the separated oligosaccharides and ferulic acid as raw materials, using lipase method to catalyze the synthesis of ferulyl oligosaccharides, different ferulyl oligosaccharide products can be obtained, and its functional effects can be conveniently stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12P19/02C12P19/12
Inventor 张晖郭晓娜王立姚惠源马坤
Owner JIANGNAN UNIV