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Use of NK cell inhibition to facilitate persistence of engrafted MHC-I negative cells

A negative cell, MHC-I technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, animal cells, extracellular fluid diseases, etc., can solve the limitation of differentiation into mesenchymal lineage cells And other issues

Inactive Publication Date: 2008-07-09
RGT UNIV OF MINNESOTA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cells are capable of differentiating into a variety of cell types of mesenchymal origin, but are clearly limited in their potential to differentiate into cells of the mesenchymal lineage

Method used

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  • Use of NK cell inhibition to facilitate persistence of engrafted MHC-I negative cells
  • Use of NK cell inhibition to facilitate persistence of engrafted MHC-I negative cells
  • Use of NK cell inhibition to facilitate persistence of engrafted MHC-I negative cells

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preparation example Construction

[0139] The preparation of polyclonal antibodies is known to those skilled in the art. See, for example, Green, et al. Production of Polyclonal Antisera, Immunochemical Protocols (Manson, ed.), page 1-5 (Humana Press); Coligan, et al. Production of Polyclonal Antisera, Rabbits, Rats Mice and Hamsters, Current Protocols in Immunology , Section 2.4.1 (1992), the above documents are incorporated by reference. For example, in order to prepare polyclonal antibodies, various host animals can be immunized by injecting purified or partially purified NK cells or their related proteins. Various adjuvants can be used according to the host species to improve the immune response, including but not limited to Freund (complete and incomplete) adjuvants, inorganic gels such as aluminum hydroxide, surfactants such as lysolecithin, pluronic polyols, and polyols. Anions, peptides, oil emulsion adjuvants, keyhole limpet hemocyanin, dinitrophenol, and possibly useful human adjuvants such as BCG (Bacil...

Embodiment 1

[0318] Materials and methods

[0319] Mouse

[0320] C57BL / 6 and recombinase activation gene-2 ​​defective (Rag2 - / - ) Mice were obtained from Jackson Laboratory (Bar Harbor, ME) and Taconic Farms (Germantown, NY). Recombinase activating gene 2 and mice with mutations in the common cytokine receptor (Rag2 - / - -IL-2Rγc - / - ) Presented by Dr. Stephen Jameson (University of Minnesota). All mice were bred under conditions without specific pathogens, fed ad libitum according to the principles of University of Minnesota Research Animal Resources, and used at 6-12 weeks of age.

[0321] NK exhausted

[0322] To deplete NK cells in the body, some mice were injected with anti-NK1.1 monoclonal antibody (hybridoma PK136, rat IgG 2a ; Provided by Dr. Koo, Rahway, NJ), and then injected twice a week for 30 days.

[0323] Bone marrow transplantation

[0324] In the transplant with or without 10 6 MAPC DL(H2 b ) Of 20×10 6 C57BL / 6(H2 b ) The day before bone marrow cells, B10.BR mice (H2 d ) C...

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Abstract

The present invention relates to the use of means for inhibiting NK cell function to increase the persistence and / or colonization of MHC-I negative cells such as MAPCs.

Description

[0001] Cross-reference [0002] This application is a partial continuation application of the U.S. application with serial number 10 / 048,757 filed on February 1, 2002, which is a U.S. national phase application of PCT / US00 / 21387 filed on August 4, 2000, and It was published in English as WO 01 / 11011 on February 15, 2001. According to 35 USC 119(e), this national phase application requires the U.S. provisional application with serial number 60 / 147,324 filed on August 5, 1999 and serial number 60 / 164,650 filed on November 10, 1999 As the basis of priority, the application text and publication text of the above-mentioned application are hereby incorporated into this specification by way of reference. [0003] This application is also a partial continuation application of the U.S. application with serial number 10 / 467,963 filed on August 11, 2003, which is a U.S. national phase application of PCT / US02 / 04652 filed on February 14, 2002, and It was published in English as WO 02 / 064748 on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A61K39/00A61K35/12A61K35/28C12N5/074
CPCA61K35/28A61K2035/122C07K16/28C12N5/0607A61P7/00A61P7/02A61P7/04A61P7/06A61P19/00A61P35/00A61P35/02A61P37/04
Inventor B·布拉扎J·多拉尔C·M·维尔法伊
Owner RGT UNIV OF MINNESOTA
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