Fixed enzyme membrane reactor, preparation thereof and method for producing biological diesel oil by the same

A technology of membrane reactors and immobilized enzymes, applied in the direction of enzyme production/bioreactors, fixed on/in organic carriers, biological raw materials, etc., can solve the problems of reducing catalytic efficiency, easy inactivation of enzymes, and increasing production costs and other issues, to achieve the effect of improving catalytic efficiency, increasing the amount of enzyme loaded, and facilitating diffusion

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of lipase to catalyze the synthesis of biodiesel has attracted more and more attention, but the high price of enzyme preparations makes it difficult to recover and reuse free enzymes or immobilized enzymes on microsphere carriers from the reaction system, which increases production costs; in addition, the required The packed bed enzyme column reactor catalyzes the alcoholysis of animal and vegetable fats and oils in a non-aqueous phase system. The inhibitory effect of organic solvents on lipase activity and the diffusion resistance of the substrate make the enzyme easily inactivated and reduce the catalytic efficiency, while hydrolysis cannot be achieved. Real-time separation of products, etc.
These problems have seriously hindered the pace of industrialization of enzymatically synthesized biodiesel
However, there is no technology to use ultrafine fiber membranes to prepare immobilized enzyme membrane reactors and use them to hydrolyze animal and vegetable oils to produce biodiesel.

Method used

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  • Fixed enzyme membrane reactor, preparation thereof and method for producing biological diesel oil by the same

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine / hydroxyethyl methacrylate terpolymer with a molecular weight of 200,000 is dissolved in dimethylformamide to form a concentration of 4wt.%. spinning solution. Under the conditions of spinning voltage of 7kV, spinneret solution flow rate of 0.1mL / h, and receiving distance of 8cm, electrospinning was carried out to make ultrafine fiber composite membrane with fiber diameter of 1000nm, and polyester non-woven fabric was used as support material. Fill it in the inner and outer double cylindrical membrane tool to form a membrane reaction device ③, as shown in the attached figure, the inner cylinder of the membrane device is called the inner side of the membrane device membrane ④, and the cavity between the inner and outer cylinders is It is called the outer side of the membrane of the membrane device ⑤. Three membrane devices are connected in series to form the first, second and third membrane reactors. Using cr...

Embodiment 2

[0031] The acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine / hydroxyethyl methacrylate terpolymer with a molecular weight of 150,000 is dissolved in dimethylformamide to form a concentration of 5wt.%. spinning solution. Under the conditions of spinning voltage of 8kV, spinneret solution flow rate of 1mL / h, and receiving distance of 10cm, electrospinning was carried out to make ultrafine fiber composite membrane with fiber diameter of 500nm, and polyester non-woven fabric was used as support material. It is filled in a roll-type membrane, and multiple membranes are connected in series to form a first-, second-, and third-stage membrane reactor. Using cross-flow filtration, first circulate epichlorohydrin in the membrane reactor device at 27°C for 1.5 hours, and then circulate the lipase solution with a concentration of 8g / L in the membrane reactor device at 27°C 1.5 hours; during the circulation process, both epichlorohydrin and lipase solution enter the inner side of ...

Embodiment 3

[0033] The acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine / hydroxyethyl methacrylate terpolymer with a molecular weight of 100,000 is dissolved in dimethylformamide to form a concentration of 4wt.%. spinning solution. Under the conditions of spinning voltage of 10kV, spinneret solution flow rate of 2mL / h, and receiving distance of 25cm, electrospinning was carried out to make ultrafine fiber composite membrane with fiber diameter of 200nm, and polyester non-woven fabric was used as supporting material. It is filled in a roll-type membrane tool, and multiple membrane tools are connected in series to form the first, second and third-stage enzyme membrane reactors. Using cross-flow filtration, first circulate epichlorohydrin in the membrane reactor device at 25°C for 2 hours, and then circulate the lipase solution with a concentration of 10g / L in the membrane reactor device at 25°C 2 hours; during the circulation process, the epichlorohydrin and lipase solution enter t...

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Abstract

The invention relates to an immobilized enzyme membrane reactor and the preparation thereof and a method for producing bio-diesel. The method comprises filling superfine fiber complex film in a die whose inside and outside are double cylindrical shape to form a film reactor, injecting activating agent solution and lipase solution in the film reactor in turn through adopting a cross-flow filtration mode, leading lipase to be chemically fixed on superfine fiber membrane surface, getting an enzyme membrane reactor which is fixed with lipase, respectively injecting mixed solution of animal and vegetable oil and carbinol in the inner side and the outer side of the superfine fiber complex film, doing catalytic alcoholysis reaction on fiber membrane surface, and producing bio-diesel. The enzyme gathering catalysis of the method of the invention is integral with a membrane separation process, and the method of the invention simplifies manufacturing technique, and meanwhile, the method of the invention overcomes inhabitation of lower alcohol to enzymatic activity in the process of catalyzing, the immobilized enzyme membrane reactor can be repeatedly used, which can achieve continuous production, improves production efficiency, and has excellent industrial application prospect.

Description

technical field [0001] The invention belongs to an immobilized enzyme membrane reactor and its preparation and application technology, in particular to a method for producing biodiesel with an immobilized enzyme membrane reactor. Background technique [0002] With the increasing depletion of petroleum resources and the enhancement of public awareness of environmental protection, biodiesel, a renewable green and environmentally friendly fuel, has attracted widespread attention from all over the world. Biodiesel is an energy source obtained from renewable biomass resources that is similar in nature to petroleum diesel. Its main components are esters such as methyl or ethyl esters formed from long-chain fatty acids. Compared with petroleum diesel, biodiesel has similar combustion performance, and biodiesel is far less corrosive to engines than petroleum diesel; biodiesel does not contain aromatic hydrocarbons and has extremely low sulfur content, which can greatly reduce pollut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40C12N11/02C10G3/00
CPCY02P30/20
Inventor 徐志康黄小军余安国
Owner ZHEJIANG UNIV
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