Fixed enzyme membrane reactor, preparation thereof and method for producing biological diesel oil by the same

Abstract

The invention relates to an immobilized enzyme membrane reactor and the preparation thereof and a method for producing bio-diesel. The method comprises filling superfine fiber complex film in a die whose inside and outside are double cylindrical shape to form a film reactor, injecting activating agent solution and lipase solution in the film reactor in turn through adopting a cross-flow filtration mode, leading lipase to be chemically fixed on superfine fiber membrane surface, getting an enzyme membrane reactor which is fixed with lipase, respectively injecting mixed solution of animal and vegetable oil and carbinol in the inner side and the outer side of the superfine fiber complex film, doing catalytic alcoholysis reaction on fiber membrane surface, and producing bio-diesel. The enzyme gathering catalysis of the method of the invention is integral with a membrane separation process, and the method of the invention simplifies manufacturing technique, and meanwhile, the method of the invention overcomes inhabitation of lower alcohol to enzymatic activity in the process of catalyzing, the immobilized enzyme membrane reactor can be repeatedly used, which can achieve continuous production, improves production efficiency, and has excellent industrial application prospect.

Description

technical field [0001] The invention belongs to an immobilized enzyme membrane reactor and its preparation and application technology, in particular to a method for producing biodiesel with an immobilized enzyme membrane reactor. Background technique [0002] With the increasing depletion of petroleum resources and the enhancement of public awareness of environmental protection, biodiesel, a renewable green and environmentally friendly fuel, has attracted widespread attention from all over the world. Biodiesel is an energy source obtained from renewable biomass resources that is similar in nature to petroleum diesel. Its main components are esters such as methyl or ethyl esters formed from long-chain fatty acids. Compared with petroleum diesel, biodiesel has similar combustion performance, and biodiesel is far less corrosive to engines than petroleum diesel; biodiesel does not contain aromatic hydrocarbons and has extremely low sulfur content, which can greatly reduce pollut...

AI Technical Summary

Problems solved by technology

The use of lipase to catalyze the synthesis of biodiesel has attracted more and more attention, but the high price of enzyme preparations makes it difficult to recover and reuse free enzymes or immobilized enzymes on microsphere carriers from the reaction system, which increases production costs; in addition, the required The packed bed enzyme column reactor catalyzes the alcoholysis of animal and vegetable fats and oils in a non-aqueous phase system. The inhibitory effect of organic solvents on lipase activity and the diffusion resistance of the substrate make the enzyme easily inactivated and reduce the catalytic efficiency, while hydrolysis cannot be achieved. Real-time separation of products, etc.

These problems have seriously hindered the pace of industrialization of enzymatically synthesized biodiesel

However, there is no technology to use ultrafine fiber membranes to prepare immobilized enzyme membrane reactors and use them to hydrolyze animal and vegetable oils to produce biodiesel.

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