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Kit for detecting femaleosteoporosis susceptible inheritance risk

A technology for osteoporosis, genetic risk, applied in the direction of determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Inactive Publication Date: 2008-08-13
SHANGHAI HENGJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene cause rickets caused by vitamin D deficiency
Currently reported VDR gene polymorphisms are mainly related to rs1544410 and rs731236 single nucleotide polymorphism sites, which can be analyzed by restriction length polymorphism, so the corresponding Bsm I and Taq I restriction enzymes of the polymorphic sites are named , which are the Bsm I(G / A) polymorphism site of the 9th intron, and the Taq I site of the 10th exon, the Taq I site is a T / C polymorphism, resulting in the coded amino acid code Sub 352ATT / ATC polymorphism

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Use of detection kits

[0029] Step 1: Extraction of DNA template

[0030] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0031] Step 2: Real-time quantitative PCR reaction

[0032] Use a fluorescent quantitative PCR detection kit for detecting the genetic risk of female osteoporosis susceptibility, which contains the following primer pairs and fluorescent probe pairs:

[0033] Sense primer 1: 5'-ACTTTTCCCCCCTAGTTG-3' (SEQ ID NO: 1)

[0034] Antisense primer 1: 5'-TGTGCAATGTGACGTC-3' (SEQ ID NO: 2)

[0035] Sense primer 2: 5'-TTACCACGAGCGCG-3' (SEQ ID NO: 3)

[0036] Antisense primer 2: 5'-GGTTTCCGGGGACC-3' (SEQ ID NO: 4)

[0037] Sense primer 3: 5'-GAGGCAATAGGTTTTGAGGG-3' (SEQ ID NO: 5)

[0038] Antisense primer 3: 5'-GGACCCTGGAGGCTGAA-3' (SEQ ID NO: 6)

[0039] Sense primer 4: 5'-GAGCAGAGCCTGAGTATT-3' (SEQ ID NO: 7)

[0040] Antisense primer 4: 5'-TTCCTGGGGCCACAGA-3' (SEQ ID NO: 8)

[0041] Sense primer 5: 5'-TGGACAG...

Embodiment 2

[0062] Example 2. Services for genetic risk detection of female osteoporosis susceptibility to people

[0063] Step 1: DNA Extraction

[0064] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0065] Step 2: Genotyping Assays

[0066] Using the kit provided by the present invention, the SNP site No. rs1800795 on the gene encoding interleukin 6, the SNP site No. rs2073618 on the gene of osteoprotegerin TNFRSF11B, and the SNP site No. rs1800629 on the gene of tumor necrosis factor-α Sites, rs1544410 and rs731236 sites on the vitamin D receptor gene were detected by fluorescence quantitative PCR to determine the genotypes of these five SNPs sites.

[0067] Step 3: Analysis of the genetic risk of female osteoporosis susceptibility

[0068] Through the analysis of the SNPs genotypes of the tested subjects,...

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PUM

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Abstract

The invention discloses an agent box for detecting female osteoporosis impressionable heredity risks. The agent comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1800795 SNP site on encode interleukins 6 gene, number rs2073618 SNP site on bone protection element TNFRSF11B gene, number rs1800629 SNP site on tumor necrosis factor-Alpha, number rs1544410 and rs731236 SNP site on vitamin D receptor gene, and general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses female osteoporosis impressionable heredity risks by detecting synchronously mononucleotide polymorphism site gene type correlative closely to osteoporosis impressionable heredity risks.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting the genetic risk of female osteoporosis susceptibility, by simultaneously detecting the white blood cells closely related to the genetic risk of female osteoporosis susceptibility Single nucleotide polymorphisms of interlein 6 gene (IL-6), osteoprotegerin gene TNFRSF11B (OPG), tumor necrosis factor-α gene (TNF-α), vitamin D receptor gene (VDR) Types to assess the genetic risk of female osteoporosis susceptibility. Background technique [0002] Osteoporosis (OP) is a metabolic disease characterized by low bone mass and microarchitectural destruction of bone tissue, resulting in increased bone fragility and susceptibility to fracture. OP can be divided into primary and secondary categories. The primary cause of secondary disease is clear, often caused by endocrine and metabolic diseases (such as hypogona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯哲民邹祖烨
Owner SHANGHAI HENGJIAN BIOTECH CO LTD
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