Vitrification frozen stock solution

A vitrification cryopreservation and solution technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of low-temperature preservation of biological materials, and achieve the goal of inhibiting recrystallization, preventing ice crystal growth, and ensuring stability Effect

Inactive Publication Date: 2008-09-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single antifreeze has its own advantages and disadvantages, and cannot meet the various requirements of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Use 0.1g of pre-prepared polyvinyl alcohol as non-permeable antifreeze, 30g of 1,2-propanediol and 30g of dimethyl sulfoxide as permeable antifreeze, and mix the three components to form a PVA / DMSO / PE system , add water to the system to form a 100ml solution, which is the vitrification solution.

Embodiment 2

[0025] 1g of pre-prepared polyvinyl alcohol is used as a non-permeable antifreeze agent, 20g of 1,2-propylene glycol and 20g of dimethyl sulfoxide are used as a permeable antifreeze agent, and the three components are mixed to form a PVA / DMSO / PE system. Add water to the system to form a 100ml solution, which is the vitrification solution.

Embodiment 3

[0027] 2g of pre-prepared polyvinyl alcohol is used as a non-permeable antifreeze agent, 10g of 1,2-propylene glycol and 10g of dimethyl sulfoxide are used as a permeable antifreeze agent, and the three components are mixed to form a PVA / DMSO / PE system. Add water to the system to form a 100ml solution, which is the vitrification solution.

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PUM

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Abstract

The invention discloses a vitrification frozen stock solution. Each 100 milliliters of the solution contains 0.1-5g of polyvinyl alcohol, 1-30g of dimethyl sulfoxide, 1-30g of 1, 2-propylene glycol and water for the rest. The inventive frozen stock solution contains a penetrability and a non-penetrability cryoprotective protective agents, particularly the polyvinyl alcohol which is taken as the non-penetrability frozen stock solution component, has obvious effects of preventing the ice crystal growth and restraining the recrystallization and is capable of effectively forming the vitrification frozen stock at low temperature. Given the factors including the toxicity of the protective agent and the penetrability, the vitrification property and stability as well as the speeds of warming and cooling, etc. the inventive frozen stock solution is subject to the vitrification state in the cooling process and further guarantees the stability of the vitrification solution in the warming process. The inventive frozen stock solution has simple and all-purpose effects and is applicable to the vitrification frozen stock of various cells, tissues and organs.

Description

technical field [0001] The invention relates to a vitrified cryopreservation solution for tissue and organ preservation. Background technique [0002] Cryopreservation of biological materials, especially tissues and organs, is one of the important goals of research in the field of cryobiology. At present, the cryopreservation of simple tissues such as cells and embryos has achieved obvious results, but the preservation of complex tissues and organs is still limited to a certain extent. The reason is that when the low temperature acts on the organism, the freezing of water will inevitably occur inside it. Whether it is intracellular or extracellular freezing, it will lead to the loss of cell activity and function, and at -15--50°C In this temperature range, a large number of ice crystals are easily formed inside and outside the cells, resulting in cell inactivation. During freezing and rewarming, cells must pass through this dangerous temperature zone twice. Therefore, eff...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 吕树申王海燕
Owner SUN YAT SEN UNIV
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