Method for preparing porcine epidemic diarrhea resisting immune globulin

A porcine epidemic diarrhea and immunoglobulin technology, which is applied in the field of medicine, can solve the problems of no report on the preparation method of porcine epidemic diarrhea serum and immunoglobulin, and achieve the effects of high titer, good safety and high cure rate.

Inactive Publication Date: 2008-09-17
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the preparation method of porcine epidemic diarrhea serum and immunoglobulin from cattle immunized with virus vaccine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of anti-porcine epidemic diarrhea immunoglobulin, the steps are as follows:

[0023] (1) Basic immunization, select a few healthy animal cows, mix the inactivated porcine epidemic diarrhea virus with tissue culture and Freund's complete adjuvant in a volume ratio of 1:1, and immunize the cows with porcine epidemic diarrhea vaccine. For the first time, 50 porcine epidemic diarrhea vaccines were injected intramuscularly at multiple points in the neck;

[0024] (2) Booster immunization is carried out 8 days after the basic immunization, and 200 porcine epidemic diarrhea vaccines are injected intramuscularly into each cow;

[0025] (3) After the booster immunization, the porcine epidemic diarrhea antibody titer reaches the highest peak after slaughtering; firstly, according to conventional regulations, the neck of the cattle to be slaughtered is partially sheared, rinsed and disinfected, and then the skin and fat are cut to fully expose the neck. Vein,...

Embodiment 2

[0031] A preparation method of anti-porcine epidemic diarrhea immunoglobulin, the steps are as follows:

[0032] (1) Basic immunization, select healthy animals, such as a few cattle, mix the inactivated porcine epidemic diarrhea virus with tissue culture and Freund's complete adjuvant in a volume ratio of 1:1, and then immunize the cattle with porcine epidemic diarrhea vaccine. , the first multi-point intramuscular injection of 30 porcine epidemic diarrhea vaccines in the neck of each cow;

[0033] (2) Booster immunization is carried out 7 days after the basic immunization, and 100 porcine epidemic diarrhea vaccines are injected intramuscularly into each cow;

[0034] (3) After the booster immunization, the porcine epidemic diarrhea antibody titer reaches the highest peak after slaughtering; firstly, according to conventional regulations, the neck of the cattle to be slaughtered is partially sheared, rinsed and disinfected, and then the skin and fat are cut to fully expose the...

Embodiment 3

[0040] A preparation method of anti-porcine epidemic diarrhea immunoglobulin, the steps are as follows:

[0041] (1) Basic immunization, select healthy animals, such as a few cattle, mix the inactivated porcine epidemic diarrhea virus with tissue culture and Freund's complete adjuvant in a volume ratio of 1:1, and then immunize the cattle with porcine epidemic diarrhea vaccine. , the first multi-point intramuscular injection of 80 porcine epidemic diarrhea vaccines per cow;

[0042] (2) Booster immunization is carried out 10 days after the basic immunization, and 300 porcine epidemic diarrhea vaccines are injected intramuscularly into each cow;

[0043] (3) After the booster immunization, the porcine epidemic diarrhea antibody titer reaches the highest peak after slaughtering; firstly, according to conventional regulations, the neck of the cattle to be slaughtered is partially sheared, rinsed and disinfected, and then the skin and fat are cut to fully expose the neck. Vein, c...

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PUM

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Abstract

The invention discloses a preparation method of immunoglobulin for resisting porcine epidemic diarrhea, which includes the following steps: (1) mixing tissue-cultured deactivated porcine epidemic diarrhea virus and Freund complete adjuvant at a volume ratio of 1:1, and performing fundamental immunity on cattle immunized with porcine epidemic diarrhea vaccine; (2) performing booster immunization after 7-10 days; (3) slaughtering and sampling blood; (4) adding penicillin and streptomycin, naturally solidifying, standing, and separating serum; (5) precipitating with ammonium sulfate to obtain the hybridprotein, and killing susceptive virus; and (6) filtering and degerming. The prepared immunoglobulin has the advantages of good therapeutic effect, good safety and reliability, no side and toxic effects, low cost, etc.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a preparation method of anti-porcine epidemic diarrhea immunoglobulin. Background technique [0002] Porcine epidemic diarrhea is a highly contagious infectious disease of pigs caused by porcine epidemic diarrhea virus. It is widespread, with high morbidity and mortality. It is extremely harmful and causes serious economic losses to the pig industry. [0003] With the development of the pig industry, the degree of intensification is getting higher and higher, and the scale is getting bigger and bigger; and with the continuous introduction of a large number of breeding pigs from abroad and the increasing trade and circulation of domestic pigs, etc., the risk of porcine epidemic diarrhea Prevention and control has brought many difficulties. Porcine epidemic diarrhea, also known as porcine epidemic viral diarrhea, is an intestinal infectious disease caused by porcine epidemic diarrhea virus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30C07K16/08
Inventor 古长庆
Owner TIANJIN SHENGJI GRP CO LTD
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