Antiviral polypeptides and use

An anti-virus and measles virus technology, applied in the field of anti-measles virus and human immunodeficiency virus anti-virus polypeptide of East Asian scorpion, and the field of high-efficiency anti-virus function polypeptide, which can solve the instability of its own genetic material and the reduction of preventive effect , prone to mutations and other problems, to achieve the effect of good water solubility, good inhibitory effect, and no side effects

Inactive Publication Date: 2008-10-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some viruses, such as influenza and HIV, have unstable genetic material and are prone to mutations, s...

Method used

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  • Antiviral polypeptides and use
  • Antiviral polypeptides and use
  • Antiviral polypeptides and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of a scorpion venom antiviral polypeptide. Proceed as follows:

[0026] A: Extraction of total RNA from scorpion venom glands (Trizol LS one-step method: Trizol LS was purchased from Invitrogen, USA)

[0027] ① Grind 500mg of scorpion glands into fine powder in liquid nitrogen, add 10ml Trizol LS to mix, and place at room temperature (20-25°C, the same below) for 5 minutes; Minutes, centrifuge at 12,000g for 15 minutes at 4°C; ③Add 1 volume of isopropanol to the water phase, place at room temperature for 10 minutes, and centrifuge at 12,000g for 10 minutes at 4°C to obtain RNA precipitation; ④Wash the precipitate with 5ml of 75% ethanol, and centrifuge at 7,500g for 5 minutes ; ⑤ After the RNA precipitate was dried, it was dissolved in DEPC (diethypyrocarbonate, diethyl pyrocarbonate)-treated sterilized water, and kept at 55-60°C for 10 minutes to completely dissolve the RNA. The whole process was carried out according to the method recommended ...

Embodiment 2

[0075] Embodiment 2: chemical synthesis of scorpion venom antiviral polypeptide AVP-W2

[0076] A polypeptide AVP-W2 consisting of 17 amino acids was isolated from the venom gland of the East Asian scorpion and was amidated. A high-purity AVP-W2 polypeptide: LVGLLPSVIGGLVSAFK-NH2 was obtained by solid-phase chemical synthesis.

Embodiment 3

[0077] Example 3: Inhibition of Measles Virus by Antiviral Polypeptide AVP-W2 Drugs

[0078] Material:

[0079] A: Culture of Vero E6 cells

[0080] The African green monkey kidney epithelial cell line (VeroE6) was purchased from China Center for Type Culture Collection.

[0081] Medium: 10% (passage medium) or 2% (maintenance medium) calf serum (FCS, GIBCO-BRL), 56 ° C, 30 minutes heat sterilization; 2mM / mL L-glutamate; 100U / mL Penicillin and 100 μg / mL streptomycin. Culture conditions are: 37°C, 5% CO 2 Incubator cultivation.

[0082] B: virus strain

[0083] The Edmonston measles virus used in this study came from the Wuhan Institute of Virology, Chinese Academy of Sciences (purchased from the American Type Culture Collection).

[0084] Specific operation method:

[0085] Monolayer Vero E6 cells were inoculated in a 96-well plate, 10 per well 4 For cells, add resistant medium to allow the cells to grow and adhere to the wall. The virus was added to the maintenance m...

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Abstract

The invention discloses scorpion toxin antiviral polypeptide and a use of the same. Scorpion toxin antivirotic polypeptide (AVP-W2) is obtained through a molecular biological and chemical synthesis method, and then the antiviral activity of the AVP-W2 on measles virus (MeV) and human immunodeficiency virus (HIV) is measured through adopting an antibody neutralization method; moreover, the measurement shows that the AVP-W2 can effectively inhibit virus infection at a low concentration. The AVP-W2 has a tremendous prospect in making applied medicine which can cure or prevent a disease caused by virus. Moreover, the antiviral peptide of the invention has high activity on MeV and HIV and a simple method, and can be developed as antiviral medicine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a polypeptide with high-efficiency antiviral function. Specifically, the present invention relates to the use of an antiviral polypeptide of the scorpion scorpion in anti-measles virus (MeV) and human immunodeficiency virus (HIV). Background technique [0002] Virus infection is a disease that seriously endangers human health, and this problem has plagued human beings for many years. As early as 412 BC in ancient Greece, Hippocrates had already described influenza-like diseases; from 1742 to 1743, influenza caused by influenza viruses had affected 90% of Eastern Europeans; from 1889 to 1894 The "Russian flu" that swept through Western Europe in 1950 had a wide range of morbidity and a high mortality rate; in 1918, a deadly flu swept the world, causing 20 to 50 million deaths; the "Asian flu" in 1957 and the "Hong Kong flu" in 1968 According to statistics in the Unit...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/12A61K38/10A61P31/14A61P31/18
Inventor 李文鑫曹志贱戴潮鄢慧民赵震环马一保
Owner WUHAN UNIV
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