Rhodobacter sphaeroides mutant strain and uses thereof

A technology of Rhodobacter sphaeroides and coenzyme, applied in the field of microorganisms, can solve the problem of low production of coenzyme Q10

Inactive Publication Date: 2008-10-15
CHINA ACADEMY OF SPACE TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, the yield of coenzyme Q10 produced by Rhodobacter sphaeroides 1.1737 is not high, and the level of shake flask fermentation usually only reaches 20-25ppm

Method used

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  • Rhodobacter sphaeroides mutant strain and uses thereof
  • Rhodobacter sphaeroides mutant strain and uses thereof
  • Rhodobacter sphaeroides mutant strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 Space mutation breeding of Rhodobacter sphaeroides mutant strain

[0051] 1. Preparation of samples carried by space breeding satellites

[0052] Take the well-growing culture of Rhodobacter sphaeroides 1.1737 and transfer it to the special tube for loading under aseptic conditions, tighten the cap of the tube, seal the interface with parafilm, put it into the large tube for loading, and store it at 4°C before sending it for loading. save. The carrying tube was placed in the experimental device of the "Shijian No. 8" space breeding satellite, and it was recovered after flying with the spacecraft in space for 15 days.

[0053] 2. The equipped space environment

[0054] During the flight of this breeding satellite, the relevant data of the space environment are: the satellite attitude control is a three-axis stable attitude, in which the I quadrant of the satellite points to the ground, and the small head of the satellite recovery module is the flight direc...

Embodiment 2

[0058] Ground screening, separation, extraction of embodiment 2 Rhodobacter sphaeroides mutant strain

[0059] 1. Ground screening

[0060] Under sterile conditions, take the recovery sample with a culture area of ​​0.5 square centimeters, elute the bacteria with 5 ml of sterile water into a sterile centrifuge tube, pipette the eluate evenly, and take 0.5 ml Add the bacterial solution to 4.5 ml of sterile water, pipette it evenly, and so on to make a doubling dilution. take 10 -4 and 10 -5 Apply the diluted solution to the plate medium, apply 100-150 microliters of the diluted bacterial solution to each plate, and apply multiple plates for each dilution gradient. After the bacterial solution is absorbed by the culture medium, culture it upside down in an incubator at 28-37°C for 2-7 days.

[0061] After the colonies grow on the plate, select the ones that become lighter or pink in color and have larger colonies to ferment with a small amount of shake flasks for preliminary...

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Abstract

The invention provides a Rhodobacter sphaeroides mutant strain, which can reduce the use of chromatography media during the production of coenzyme Q 10 by fermentation, and consequently reduce the production cost. The mutant strain can also improve the output of THE conezyme Q10.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a mutant strain of Rhodobacter sphaeroides. The present invention also relates to the application of the mutant strain in the production of coenzyme Q10. Background technique [0002] Rhodobacter sphaeroides 1.1737 can be used to produce coenzyme Q10. However, when coenzyme Q10 is extracted from the fermentation broth of this bacterium, it is necessary to use chromatography to adsorb water-soluble substances such as pigments. Since the fermentation broth of this bacterium is dark red, a relatively large amount of chromatography packing material is consumed for decolorization, and the amount of packing material used in chromatography is related to the cost of producing coenzyme Q10. If the fermented broth of Rhodobacter sphaeroides with a lighter color can be obtained, the amount of chromatographic filler can be reduced and the production cost of coenzyme Q10 can be directl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/66C07C46/10C12R1/01
Inventor 印红庞欣党磊靳明慧章文荻
Owner CHINA ACADEMY OF SPACE TECHNOLOGY
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