Method for separating and preparing salidroside

A technology for salidroside and ethanol, which is applied in the field of preparation of salidroside separation, can solve the problems of inability to large-scale production, low product purity, organic residues and the like, and is suitable for industrialized large-scale production and has high sample purity. , the effect of low organic residues

Active Publication Date: 2011-04-20
浙江华谱新创科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

The reports about the extraction and separation preparation of salidroside in the literature basically all adopt methods such as traditional solvent extraction, lead acetate precipitation, silica gel chromatography, and activated carbon adsorption (Wang Wei et al. Chinese herbal medicine, 1999, 30 (11): 824 -826; Xian Mo et al. Journal of Natural Sciences of Jilin University, 1998, 3: 107-108; Lu Xixian et al. Chinese Herbal Medicine, 1980, 4, 147-148; ), these methods have shortcomings such as low product purity, poor reproducibility, long time consumption, serious organic residues, and inability to produce on a large scale

Method used

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Examples

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Effect test

Embodiment 1

[0022] Crush the original medicinal material of Rhodiola rosea, weigh 1 kg quantitatively, put it in a 50-liter extraction tank, add 10 liters of water to decoct for 2 hours, filter, and save the filtrate for later use, add 10 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 240 ml of extract with a relative density of 1.12. Add 412 milliliters of 95% ethanol to the extract, stir well, leave it in a refrigerator at 0°C for 24 hours, filter, discard the filter residue, and concentrate the filtrate to 160 milliliters, relative density 1.05, add 854 milliliters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20...

Embodiment 2

[0031] Crush the original medicinal material of Rhodiola rosea, weigh 0.5 kg quantitatively, put it in a 20-liter extraction tank, add 6 liters of water to decoct for 1 hour, filter, and save the filtrate for later use, add 5 liters of water to the filter residue and decoct for 3 hours, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 140 ml of extract with a relative density of 1.10. Add 240 milliliters of 95% ethanol to the extract, fully stir, refrigerate and stand for 24 hours at 0°C, filter, discard the filter residue, and concentrate the filtrate to 40 milliliters, relative density 1.07, add 428 milliliters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20,000 r...

Embodiment 3

[0035] Crush the original medicinal material of Rhodiola rosea, weigh 5 kg quantitatively, put it in a 250-liter extraction tank, add 60 liters of water to decoct for 2 hours, filter, and save the filtrate for later use, add 60 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 1.2 liters of extract with a relative density of 1.13. Add 2.1 liters of 95% ethanol to the extract, stir fully, and refrigerate at 0°C for 24 hours, filter, discard the filter residue, and concentrate the filtrate to 0.7 liters, relative density 1.08, add 3.8 liters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20,000 rpm) to obtain ...

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Abstract

The invention relates to the separation of natural medicines, in particular to a separation and preparation method of the salidroside; wherein, extracting solution is obtained by decocting the ground rhodiola with ten times of water, and then the extract (the relative density is from 1.10 to 1.15) is obtained after the extracting solution is concentrated, and then the ethanol is added for twice of alcohol precipitation, and then the eluate is collected by separating the solution with a 6000a molecular weight membrane separator and an efficient industrial chromatographic column and eluting themethanol water with gradients and then the salidroside is obtained by collecting, concentrating, freezing and drying the eluate. The method is high in product purity, large in preparation amount and stable and reliable in process and especially suitable for separating and preparing a great amount of highly purified salidroside compounds from the traditional Chinese medicine rhodiola.

Description

technical field [0001] The invention relates to the separation of natural medicines, in particular to a preparation method for separating salidroside from traditional Chinese medicine rhodiola rosea through industrial chromatographic technology. Background technique [0002] Rhodiola is a perennial herb or subshrub of the genus Rhodiola (Rhodio la L.) in the Sedum family. It often has fleshy, creeping rhizomes. There are more than 90 species in the world, and there are more than 70 species in China. Folks are mostly used to treat hemoptysis, hemoptysis, cough due to lung heat, bruises, burns and scalds. In recent years, many new pharmacological effects have been discovered through research. Rhodiola rosea has biomedical effects such as anti-hypoxia, anti-aging, anti-fatigue, anti-adverse stimuli, two-way regulation of the body, anti-virus and anti-tumor, and lowering blood lipids (VA Kurk , PharmChem J, 1986, 20(10): 1237; Yin Guishan et al. Journal of Nutrition, 1992, 14(1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18A61K36/41
Inventor 梁鑫淼丰加涛徐青
Owner 浙江华谱新创科技有限公司
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