Persimmon leaf flavones extract and preparation thereof

Abstract

The invention discloses a flavonoid extract from persimmon leaves and a preparation method thereof, the method comprises the steps as follows: the persimmon leaves are crushed, and extracted for once to three times by low carbon alcohol with each time lasting for 5-180min, the extracting solution is mixed, the low carbon alcohol is recovered, condensed and centrifuged, the supernatant is purified by macroporous resin column, the column is washed with water, and then with the low carbon alcohol, the eluent is collected, after the eluent is condensed and the low carbon alcohol is recovered, the eluent is extracted for two to five times by an organic reagent, the extracting solution is condensed and dried, thus obtaining the flavonoid extract from the persimmon leaves. The method adopts the processes of alcohol extraction, macroporous resin purification and ethyl acetate extraction to extract flavonoid substances from the persimmon leaves, overcomes the defects of low yield and low content of flavonoid substances from the persimmon leaves in the prior art, simplifies the production process, has easy operations, reduces production cost, extracts effective components fully and avoids the loss of effective components; the content of the flavonoid substances in the extract is high, with the weight content up to 80-98%.

Description

(1) Technical field: [0001] The invention relates to a flavonoid extract from persimmon leaves and a preparation method thereof, belonging to the technical field of extraction of medicinal active ingredients. (two) background technology: [0002] The main active ingredients in persimmon leaves are flavonoids. The flavonoids found in persimmon leaves include astragaloside, isoquercetin, kaempferol, quercetin, kaempferol-3-o-β-D-glucoside, and kaempferol -3-o-α-L-rhamnoside, kaempferol-3-β-D-xyloside, kaempferol-3-o-α-L-arabinoside, quercetin-3-o- [2″-o-(3,4,5-trihydroxybenzoyl)]-glucoside, rutin, hyperoside, bayberry bark bark glycoside, astragalus glycoside, etc. Domestic and foreign studies have shown that persimmon leaves Flavonoids have the functions of lowering blood pressure, inhibiting bacteria, lowering blood fat, lowering cholesterol, stopping bleeding, promoting blood circulation and removing blood stasis, inhibiting tumor growth, and promoting plant growth. [00...

AI Technical Summary

Problems solved by technology

(2) Extract by boiling water, add alkaline substances to help dissolve, filter, and then elute on the filtrate to collect the eluate; what this method obtains is also a crude extract, although it has the function of lowering blood pressure and promoting Growth activity, but there are still many impurities, which cannot be used as medicinal ingredients for human body

(3) CN1709542A discloses a method for extracting flavonoids from persimmon leaves by two-phase extraction. First, add ethanol solution for solid-liquid leaching, then carry out two-phase extraction for the crude extract, then back-extract with acetates, distill, and dry. but the content of flavonoids in the method gained product also only has about 12.1%

However, the process of this method is cumbersome, and the extraction effect is still not ideal.