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Method for producing biological organic acid by using dynamic expanding adsorption reactor system

An organic acid and reactor technology, which is applied in the field of bio-organic acid production in a dynamic expansion adsorption reactor system, can solve the problems of low raw material utilization rate, low product yield, production efficiency decline, etc., and achieve increased product output, high biomass The effect of improving and restoring growth vitality

Inactive Publication Date: 2011-02-09
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention can overcome the phenomenon of feedback inhibition that often occurs when metabolites accumulate to a certain concentration in the process of using microbial cells or directly using enzymes to carry out catalytic reactions to produce certain products, resulting in low product yields and low raw material utilization. Problems of low production efficiency

Method used

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  • Method for producing biological organic acid by using dynamic expanding adsorption reactor system
  • Method for producing biological organic acid by using dynamic expanding adsorption reactor system
  • Method for producing biological organic acid by using dynamic expanding adsorption reactor system

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Embodiment 1. Application of dynamic expansion type adsorption reactor system to produce propionic acid / VB12

[0053] See figure 1 . The dynamic expansion adsorption reactor system used includes a fermenter 1 with a stirring device, a pipeline 2, an eluent storage tank 3, an eluent storage tank 4, a regeneration liquid storage tank 5, an equilibrium liquid storage tank 6, and a solenoid valve 7, 9, 10, 11, 12, 13, 14, 15 and 23, liquid pump 8, chromatography column 16, 17 and 18, product storage tank 19, waste liquid storage tank 20, detector 21, pipeline 22, Conversion tank 24 and so on.

[0054] The bottoms of the three parallel expanded bed chromatography columns 16, 17, 18 are all provided with circulating fluid inlets, and the tops of the expanded bed chromatography columns are all opened with circulating fluid outlets.

[0055] A circulating fluid main pipeline 22 forming a circulating fluid circuit has three three-way solenoid valves 12, 13 and 14 and four fou...

Embodiment 2

[0081] Example 2. Production of lactic acid using a dynamic expansion adsorption reactor system.

[0082] Utilize the production process adopted in the production of lactic acid by dynamic expansion type adsorption reactor, and the applied dynamic expansion type adsorption reactor system used is basically the same as embodiment 1, the difference is:

[0083] (1) The microbial cell seed is: Lactobacillus plantarum.

[0084] (2) The N source in the seeds and fermentation medium was changed from corn steep liquor to peptone, the mass concentration of peptone in the seed medium was changed to 2%, and the mass concentration of peptone in the fermentation medium was changed to 2%.

[0085] (3) The fermentation process is changed to an aerobic mode.

[0086] (4) Since there is only one target product of lactic acid in this embodiment, it enters the fermentation process, and the link of adding inducers during the growth of microbial cells to the late logarithmic period is omitted dur...

Embodiment 3

[0090] Example 3. Production of succinic acid using a dynamic expansion adsorption reactor system.

[0091] Utilize the production technique adopted in the production of succinic acid by dynamic expansion type adsorption reactor, and the applied dynamic expansion type adsorption reactor system used is basically the same as embodiment 1, difference is:

[0092] (1) The microbial cell seed is: Actinobacillus succinate.

[0093] (2) The N source in the seeds and fermentation medium was changed from corn steep liquor to yeast powder, the mass concentration of yeast powder in the seed medium was changed to 2%, and the mass concentration of yeast powder in the fermentation medium was changed to 2%.

[0094] (3) Since there is only one target product, succinic acid, in this embodiment, it enters the fermentation process, and the link of adding inducers during the growth of microbial cells to the late logarithmic period during the fermentation process is omitted.

[0095] Experimenta...

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Abstract

The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for producing biological organic acid by using a dynamic expanding adsorption reactor system. In a microbial cell fermentation process, a biological organic acid target product is secreted in extracellular fermentation solution together with the growth of microbial cells, an enzyme system participating in the microbial cell growth metabolism is subjected to feedback suppression of the biological organic acid target product at the moment so that the metabolism velocity of the cells is reduced, and the fermentation solution with the microbial cells is directly pumped into an expanded bed chromatographic column filled with a separation medium in a dynamic expanding adsorption reactor so as to realize on-line adsorption of the biological organic acid target product through certain adsorption and separation processes and avoid plugging the chromatographic column; and the fermentation solution from which the biological organic acid target product is removed can be circularly refluxed to a fermentation tank. The suppression on the growth of the microbial cells is relieved, the growth vitality of the microbial cells is recovered, the biomass of the microbial cells is improved, and the yield of the biological organic acid product is increased.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for producing biological organic acids using a dynamic expansion adsorption reactor system. Background technique [0002] One of the difficulties encountered in the production of various products by microbial cell fermentation is the feedback inhibition of cell metabolites. The phenomenon is that during the fermentation process of microbial cells, the bacteria grow and reproduce with specific substrates as nutrients and at the same time enter the cells or Extracellular secretion of metabolites. After the concentration of some cell metabolites reaches a certain range, the target product will no longer increase, but the impurities will increase greatly, resulting in the inability to increase the fermentation yield. At the same time, a large amount of impurities will interfere with the subsequent separation. If the feedback substances are remove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/225C12P7/44C12R1/19C12P7/48C12R1/25C12P7/46C12P7/52C12P7/54C12R1/01
Inventor 王云山王鹏苏志国李凌云张建飞
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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