Bacillus subtilis for efficiently degrading deoxynivalenol and application of bacillus subtilis

A technology of Bacillus subtilis and DON, applied in the field of microbial degradation, can solve the problems of poor effect, secondary pollution, no effective control or eradication of toxins, etc., and achieves the effect of stable metabolism and high growth vitality.

Inactive Publication Date: 2020-10-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many research reports on the biochemistry, toxicity and mode of action of vomitoxin, but there is still no feasible way to effectively prevent or eradicate these toxins
Although physical and chemical methods are often used to decontaminate DON in feed, such as heat treatment, gravity sorting and cleaning, these detoxification methods not only have low efficiency and poor effect, but also may cause secondary pollution, affect taste, Reduce the nutritional value of food, etc., so "biological detoxification" has begun to receive widespread attention

Method used

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  • Bacillus subtilis for efficiently degrading deoxynivalenol and application of bacillus subtilis
  • Bacillus subtilis for efficiently degrading deoxynivalenol and application of bacillus subtilis

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Embodiment Construction

[0024] For further illustrating the present invention, specifically illustrate in conjunction with following examples:

[0025] In this example, through multiple isolation and screening, a strain of Bacillus subtilis that can efficiently degrade vomitoxin was isolated from the natural environment, named N-22 (CGMCC No: 19864), which was obtained from wheat grains infected with scab. separated and screened. Specifically, the isolation and screening method of the bacterial strain of the present invention includes:

[0026] (1) Take 1g of wheat grains contaminated by scab and put them in a test tube, add 9ml of distilled water, incubate at room temperature for 12 hours, shake the test tube two to three times during the incubation;

[0027] (2) Take 1 ml of the incubated solution and add it to LB medium containing 200 mg / L vomitoxin, and enrich and culture it for 7 days at 30°C and 220 rpm;

[0028] (3) Dilute the enriched bacterial solution to 10% of the original concentration ...

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Abstract

The invention relates to a bacillus subtilis N-22 strain for efficiently degrading deoxynivalenol. The N-22 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC),and the preservation number is CGMCC No:19864. The bacillus subtilis N-22 disclosed by the invention is high in growth vitality and stable in metaboilic level, and can be widely applied to the fieldsof feed production, food processing and the like. The separated deoxynivalenol degrading microorganism N-22 has quite important effects on researching and developing biological detoxification agents of the deoxynivalenol, producing feeds and processing foods.

Description

technical field [0001] The invention relates to the technical field of microbial degradation, in particular to a strain of Bacillus subtilis capable of efficiently degrading vomitoxin and its application. Background technique [0002] Deoxynivalenol (DON), also known as vomitoxin, is a trichothecene toxin mainly produced by Fusarium graminearum and Fusarium pink. Its chemical name is 3α, 7α, 15-trihydroxy-12, 13-epoxytrichothecene-9-en-8-one, and its chemical formula is C 15 h 20 o 6 , the pure product is a colorless needle crystal with high thermal and chemical stability. DON can contaminate a variety of grains, such as wheat, rye, oats, corn, etc., and can also contaminate feed ingredients and full-price compound feeds (Li Xiaoying et al., 2012). DON has chronic and subchronic toxicity (Andretta I et al., 2012), acute toxicity (Huo Xinghua et al., 2008), immunotoxicity (Mikami O et al., 2010), neurotoxicity (Fitzpatrick DW et al., 1988) It can affect the digestive sys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23L5/20C12R1/125
CPCA23L5/28C12N1/02C12N1/20C12N1/205C12R2001/125
Inventor 李广悦李琲琲任杰杨秀芬曾洪梅袁京京
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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