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Method for preparing compound microbial preparation for degrading 'three-benzene' VOCs exhaust air

A technology of compound microbial bacterial agent and triphenyl, which is applied in the field of preparation of compound microbial bacterial agent, can solve the problem that the compound microbial bacterial agent has not yet been retrieved, and achieve the effects of being more conducive to long-term preservation, enriching microbial populations, and reducing the removal rate

Inactive Publication Date: 2011-02-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching the relevant patent literature, no compound microbial agent applied to the "triphenyl" VOCs waste gas biological treatment system has been retrieved.

Method used

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  • Method for preparing compound microbial preparation for degrading 'three-benzene' VOCs exhaust air

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Sample collection: Activated sludge was taken from the aeration tank of a sewage plant, stored in a refrigerator at 4°C, and used within 48 hours.

[0020] (2) Activated sludge acclimation medium: 800mg / L benzene, 500mg / L toluene, 300mg / L o-xylene, K 2 HPO 4 2.0mg / L, Na 2 HPO 4 5.0mg / L, KH 2 PO 4 1.5mg / L, NH 4 Cl 4.5mg / L, MgSO 4 6.5mg / L, 4.0mg / L CaCl 2 , 0.25mg / L FeCl 3 , MnSO 4 0.05mg / L, ZnSO 4 0.01mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.01mg / L.

[0021] (3) Activated sludge acclimation method: Settling the fresh sludge for 30 minutes, taking 50ml of thick sludge into a 500ml Erlenmeyer flask, and adding 100ml of acclimation medium. The Erlenmeyer flask was placed in a shaker at 28°C and 160 rpm for shaking culture. Every 5 days was an acclimatization cycle, and the pH of the acclimatization system was measured every other day in a cycle, and adjusted to 6.0-8.0. After the end of each cycle, replace the fresh medium and add the same concentration o...

Embodiment 2

[0026] (1) Sample collection: Same as Example 1.

[0027] (2) Activated sludge acclimation medium: benzene 1000mg / L, toluene 1000mg / L, o-xylene 500mg / L, K 2 HPO 4 6.0mg / L, Na 2 HPO 4 8.0mg / L, KH 2 PO 4 4.0mg / L, NH 4 Cl 6.0mg / L, MgSO 4 8.5mg / L, 8.0mg / L CaCl 2 , 1.0mg / L FeCl 3 , MnSO 4 0.5mg / L, ZnSO 4 0.1mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.1mg / L.

[0028] (3) Activated sludge domestication method: with embodiment 1.

[0029] (4) Preparation of microbial agent carrier: wheat bran, mahogany chips and diatomaceous earth are mixed by 85%: 10%: 5%, and water is added in the amount of carrier: water (adjusted to 7) by the amount of carrier: water (pH is adjusted to 7), after mixing Divide, add 50 grams of carrier to each 500ml Erlenmeyer flask, sterilize with damp heat at 121°C for 30 minutes, then cool down for use.

[0030] (5) Preparation process of compound microbial bacterial agent: inoculate 10ml of domesticated mixed strains in the carrier prepared in the...

Embodiment 3

[0033] (1) Sample collection: Same as Example 1.

[0034] (2) Activated sludge acclimation medium: 800mg / L benzene, 800mg / L toluene, 400mg / L o-xylene, K 2 HPO 4 4.0mg / L, Na 2 HPO 4 6.5mg / L, KH 2 PO 4 4.0mg / L, NH 4 Cl 5.0mg / L, MgSO 4 7.0mg / L, 6.0mg / L CaCl 2 , 0.7mg / L FeCl 3 , MnSO 4 0.2mg / L, ZnSO 4 0.05mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.03mg / L.

[0035] (3) Activated sludge domestication method: with embodiment 1.

[0036] (4) Bacteria agent carrier preparation: wheat bran, cellulose and stabilizer are mixed by 80%: 15%: 5%, add water (pH is adjusted to 8) by carrier: the amount of water is 1: 2, divide after mixing For each 500ml Erlenmeyer flask, add 50 grams of carrier, sterilize with damp heat at 121°C for 30 minutes, and then cool it down for use.

[0037] (5) Preparation process of compound microbial bacterial agent: inoculate 15ml of domesticated mixed strains in the carrier prepared in the above (2), solid fermentation culture at 45°C for 48h, and...

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Abstract

The present invention discloses a method for producing the compound micro-organism microbial inoculum that is used for degrading the waste gas of VOCs. Firstly, culture medium containing the triphenyl substance is adopted to naturalize the active mud that is taken from a sewage treatment plant; the active dirt that is obtained in the domestication way and has high degrading performance is used asthe mixed strain to be inoculated to the microbial inoculum carrying material, and the compound micro-organism microbial inoculum can be obtained after the solid fermentation culture. The preparing method comprises the steps of domestication of the active mud, mixing of the microbial inoculum carrying material at a giving ratio and the solid fermentation culture, so that the preparing method is simple, and the cost is low, and the advantages that the species group of the active mud mixture micro-organism is rich, the bacteria group structure is reasonable, and the microbial inoculum favors the long-term storage are effectively combined. Since the preparing method is used for preparing the target pollutant, the domestication time in the actual application of the waste-gas organism treatment can be shortened, and the rapid startup of the reaction device can be accelerated. The active mud rapid-startup waste-gas organism purification reactor can be substituted, so as to realize the high-efficient degrading of the target pollutant.

Description

technical field [0001] The invention relates to waste gas purification technology, in particular to a preparation method of a composite microbial bacterial agent for degrading "triphenyl" VOCs waste gas. Background technique [0002] Air pollution is one of the most prominent environmental problems at present. Volatile organic compounds (VOCs) are the most extensive and diverse air pollutants except dust. According to previous studies and related pollution source investigation reports, among the actual VOCs emitted, industrial "triphenyl" (benzene, toluene, xylene) VOCs account for a relatively large proportion, which is a major type of volatile organic pollutants and has been recognized by many The country has included pollutants in priority control. A large amount of "triphenyl" waste gas discharged into the atmosphere will inevitably cause harmful effects on the atmospheric environment and human health. It is very important and urgent to develop the pollution control t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D53/84B01D53/72C12N1/00
CPCY02A50/20
Inventor 陈建孟张丽丽吴石金王家德叶峰
Owner ZHEJIANG UNIV OF TECH
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