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Detection kit for oral cavity mucous membrane tissue canceration trend detection and its use method

A technology of oral mucosa and kits, applied in the field of molecular biology, can solve problems such as difficult diagnosis of lesions, and achieve the effect of short judgment time and high accuracy

Inactive Publication Date: 2011-05-11
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the overlapping and alternation of lesions, sometimes it is difficult to diagnose the lesions, therefore, it is necessary to make a therapeutic diagnosis clinically.

Method used

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  • Detection kit for oral cavity mucous membrane tissue canceration trend detection and its use method
  • Detection kit for oral cavity mucous membrane tissue canceration trend detection and its use method
  • Detection kit for oral cavity mucous membrane tissue canceration trend detection and its use method

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Embodiment Construction

[0032] 1. Reagents and instructions included in the kit

[0033] The kit of the present invention contains 12 kinds of reagents, namely TRIZOL, chloroform, isopropanol, ethanol, Oligo(dT) 15 , Reagent I, Reagent II, Reagent III-a, Reagent III-b, Reagent III-c, Reagent III-d, and Reagent III-e. Among them, TRIZOL, chloroform, isopropanol, and ethanol are reagents for extracting total RNA from tissues, and Oligo(dT) 15 And reagent I is the cDNA reagent for reverse transcription of mRNA, reagent II is the PCR operation reagent of TaKaRa company, reagent III-a is the PCR primer of gene NF-1, reagent III-b is the PCR primer of gene ACP-2, reagent III- c is the PCR primer of the gene CTNN-B1, reagent III-d is the PCR primer of the gene SOCS-3, and reagent III-e is the PCR primer of the gene β-actin.

[0034] Details are shown in Table 1', wherein the specific components of Reagent I, Reagent II and Reagent III are shown in Tables 2'-4'.

[0035] Table 1': Reagents included in the...

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Abstract

The invention belongs to the field of molecular biology, in particular to a kit used for detecting the canceration trend of an oral mucosa tissue as well as the usage thereof. The invention realizes the detection and judging on the clinic diagnosing for the canceration trend of the oral mucosa tissue. The kit used for detecting the canceration trend of an oral mucosa tissue is characterized by consisting of the following reagents: TRIZOL, chloroform, isopropanol, ethanol, Oligo(dT)15, a reagent I, a reagent II, a reagent III-a, a reagent III-b, a reagent III-c and a reagent III-d. The the usage thereof is described as follows: preparing and storing a sample; the RT-PCR operation on each gene and agarose gel electrophoresis; carrying out quantitative analysis on the result of the agarose gel electrophoresis and judging the canceration trend of the oral mucosa tissue. The invention has the following beneficial effects of being capable to simultaneously carry out the RT-PCR reaction for five genes of NF-1, ACP-2, CTNN-B1, SOCS-3 and Beta-actin by using the reagents provided by the kit and being capable of simultaneously judging the canceration degree of a plurality of oral mucosa tissue samples; the judging time is short; the accuracy is high.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit for detecting canceration tendency of oral mucosa tissue and a use method thereof. Background technique [0002] Oral mucosal disease is a general term for various types of diseases that occur on the oral mucosa and soft tissues [Li Bingqi, Zhou Zengtong. Oral Mucosal Diseases (Second Edition). Beijing: People's Health Publishing House .2005, 1-15]. Oral mucosal disease has the characteristics that different types of damage can occur in different stages of the same lesion, that the same disease has different clinical manifestations in different parts of the oral mucosa, and that multiple mucosal diseases coexist. The reasons for the many unclear features. At the same time, oral leukoplakia (OLK), oral erythema (OEK), oral lichen planus (OLP), oral submucous fibrosis (OSF), epithelial dysplasia and other oral mucosal diseases are Precancerous lesions of oral s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈显久王军赵文军刘玮玮解军牛勃程牛亮赵荣瑞郭巍伟郭剑津
Owner SHANXI MEDICAL UNIV