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Triterpene saponins compounds extracted from ardipusilloside

A technology for triterpenoid saponins and compounds, which is applied in the application field of treating glioma, can solve the problems of insufficient research on triterpenoid saponins, no discovery of anti-glioma activity of triterpenoid saponins, etc.

Inactive Publication Date: 2009-03-11
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 1993 has reported the structure and extraction and separation of eugenon saponin I and eugenon saponin II, compared with the large amount of triterpenoid saponin chemical components that have been isolated from other plants of the genus Auranthus genus, the three The research on terpene saponins is not enough, and there is no report that these three triterpene saponins have anti-glioma activity

Method used

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  • Triterpene saponins compounds extracted from ardipusilloside
  • Triterpene saponins compounds extracted from ardipusilloside
  • Triterpene saponins compounds extracted from ardipusilloside

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Embodiment 1

[0021] The extraction and separation technological process of embodiment compound are as figure 1 As shown, it collects the whole grass of Jiujielong in Jiajiang County, Sichuan as a raw material. After the raw material is crushed into a coarse powder, take 3 kg, add 10 liters of ethanol with a concentration of 95% for reflux extraction, and extract 3 times in total. 2 hours. The extracts were combined, and the solvent was recovered under reduced pressure (recovery under reduced pressure is the suction filtration method commonly used in this industry, the same below), to obtain 120 grams of ethanol extract. The extract was dispersed in 1 liter of water, and extracted 3 times with petroleum ether, 1 liter each time. The extracted aqueous phase was extracted 3 times with n-butanol, 1 liter each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 10 grams of total saponin extract. The total saponins were subjected to silica...

Embodiment 2

[0026]The whole herb of Jiujielong in Jiajiang County, Sichuan Province was collected as a raw material. After the raw material was crushed into a coarse powder, 1 kg was taken, and 4 liters of methanol was added for reflux extraction. A total of 3 extractions were performed for 3 hours each time. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 30 g of methanol extract. The extract was dispersed in 200 ml of water, and extracted 3 times with 200 ml of petroleum ether. The extracted aqueous phase was extracted 3 times with n-butanol, 200 ml each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 3 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and gradient elution was carried out with a mixed solvent of chloroform-methanol-water, and the volume ratios of the eluents were respectively 7:2:1, 7:3:1 and 6.5:3.5:1 (both were take...

Embodiment 3

[0028] The whole herb of Jiujielong in Jiajiang County, Sichuan Province was collected as a raw material. After the raw material was crushed into coarse powder, 1 kg was taken, and 5 liters of ethanol with a concentration of 95% was added for reflux extraction. A total of 3 extractions were performed for 4 hours each time. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 32 g of ethanol extract. The extract was dispersed in 160 ml of water, and extracted 3 times with 160 ml of petroleum ether. The extracted aqueous phase was extracted 3 times with n-butanol, 160 ml each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 3.5 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and gradient elution was carried out with a mixed solvent of chloroform-methanol-water, and the volume ratios of the eluents were 7:3:1 and 6.5:3.5:1 respecti...

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Abstract

The invention discloses a novel spxiterpenoid saponin type compound extracted from Ardisia pusilla and the application thereof in treating glioma. A chemical structure of the compound is determined by adopting such spectroscopic technologies as high-resolution mass spectrum and two-dimensional nuclear magnetic resonance spectroscopy and the like as well as a chemical method. An in vitro anti-tumor experiment shows that the compound has remarkable effect on inhibiting four human malignant glioma cells comprising U87MG, U251MG, BT325 and SHG44 without affecting the growth of the primary cultured human neuroglial cells. The novel spxiterpenoid saponin type compound is expected to be a drug for curing glioma.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a novel triterpene saponin compound extracted and separated from Jiujielong, its preparation method and its application in treating glioma. Background technique [0002] Human malignant glioma is the most common type of intracranial tumor. The development of new safe and efficient chemotherapy drugs is an urgent requirement for the treatment of this disease. Our previous studies have shown that the total saponins of the traditional Chinese medicine Jiujielong can inhibit human malignant glioma. Growth of plasmoma U251MG cells. Jiujielong (Ardisia pusilla A.DC) is a plant of the genus Ardisia pusilla, which is distributed in southern China. It has the effect of clearing away heat and detoxification, and is a whole herb for medicinal purposes. Plants of the genus Auranthus mainly contain triterpenoid saponin compounds, which have been proven to be the main active ingredients. Some...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61K36/185A61P35/00
Inventor 汤海峰张淑瑜田冶王晓娟文爱东
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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