Method and apparatus for judging the presence or absence of cancer cell

A cancer cell, cell technology, applied in measurement devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of different diagnosis results and long inspection time.
CN101392291AInactive Publication Date: 2009-03-25SYSMEX CORP
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SYSMEX CORP
Publication Date
2009-03-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for judging whether cancer cells are present or not in a sample, comprising a obtaining step of obtaining values related to an expression level of a caner marker gene and a housekeeping gene; a first comparing step of comparing the value related to the expression level of the cancer marker gene with a first threshold value; a normalizing step of normalizing the value related to the expression level of the cancer marker gene based on the value related to the expression level of the housekeeping gene; a second comparing step of comparing the normalized value with a second threshold value; and a judging step of judging whether cancer cells are present or not in the sample based on comparison results obtained in the first and second comparing steps, as well as an apparatus for judging whether cancer cells are present or not.
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Description

Technical field:

[0001] The present invention relates to a method and an apparatus for judging whether there are cancer cells in a sample containing cells collected from a patient, especially a patient suspected of cancer metastasis. Background technique:

[0002] Examination of the presence or absence of cancer cells in a sample collected from a patient can be used as an indicator for judging whether cancer cells have metastasized to the tissues and organs collected from the sample. In order to determine whether there are cancer cells in the sample, histiocytic diagnostic methods such as Papanicolaou staining have been used in the past. However, this method has problems such as that the diagnostic results vary depending on the experience of the diagnostician, and the examination takes a long time.

[0003] Recently, researches on cancer molecular pathological examination using LAMP (loop-mediated isothermal amplification method) method and PCR (polymerase chain reaction, p...

Claims

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