Method and apparatus for judging the presence or absence of cancer cell

A cancer cell, cell technology, applied in measurement devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of different diagnosis results and long inspection time.

Inactive Publication Date: 2009-03-25
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has problems such as the difference in diagnostic re...

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  • Method and apparatus for judging the presence or absence of cancer cell
  • Method and apparatus for judging the presence or absence of cancer cell
  • Method and apparatus for judging the presence or absence of cancer cell

Examples

Experimental program
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Embodiment

[0183] The following examples illustrate the present invention in more detail, but the present invention is not limited by the following examples.

[0184] (1) Collection of peritoneal washings and sample preparation

[0185] When performing operations on 50 patients with gastric cancer, the peritoneal washing fluid was collected for the examination of gastric cancer metastasis. Specifically, after laparotomy, 100 mL of normal saline was injected into the abdominal cavity (under the liver, under the left diaphragm and / or the douglas fossa) for flushing, and the solution was recovered. Centrifuge the recovered peritoneal washings at 3,000×g for 10 minutes, remove the supernatant, and obtain a sample of about 50-100 μL.

[0186] The 50 patients were composed of 17 patients with gastric cancer metastasis diagnosed by cell tissue and 33 patients without diagnosis of metastasis.

[0187] (2) RT-PCR

[0188] The sample prepared in (1) was treated with RNeasy Mini Kit (manufactu...

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PUM

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Abstract

The invention provides a method for judging whether cancer cells are present or not in a sample, comprising a obtaining step of obtaining values related to an expression level of a caner marker gene and a housekeeping gene; a first comparing step of comparing the value related to the expression level of the cancer marker gene with a first threshold value; a normalizing step of normalizing the value related to the expression level of the cancer marker gene based on the value related to the expression level of the housekeeping gene; a second comparing step of comparing the normalized value with a second threshold value; and a judging step of judging whether cancer cells are present or not in the sample based on comparison results obtained in the first and second comparing steps, as well as an apparatus for judging whether cancer cells are present or not.

Description

Technical field: [0001] The present invention relates to a method and an apparatus for judging whether there are cancer cells in a sample containing cells collected from a patient, especially a patient suspected of cancer metastasis. Background technique: [0002] Examination of the presence or absence of cancer cells in a sample collected from a patient can be used as an indicator for judging whether cancer cells have metastasized to the tissues and organs collected from the sample. In order to determine whether there are cancer cells in the sample, histiocytic diagnostic methods such as Papanicolaou staining have been used in the past. However, this method has problems such as that the diagnostic results vary depending on the experience of the diagnostician, and the examination takes a long time. [0003] Recently, researches on cancer molecular pathological examination using LAMP (loop-mediated isothermal amplification method) method and PCR (polymerase chain reaction, p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12M1/34C12N15/09C12Q1/04G16B25/10
CPCC12Q1/6886G06F19/20C12Q2600/158G01N33/574G16B25/00G16B25/10
Inventor 大友泰裕中林一树庄司佳代高田英基
Owner SYSMEX CORP
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