Method for preparing retina neural ganglia progenitor cells

A technique for retinal ganglion and neural precursor cells, which is applied in the field of preparing retinal ganglion-like precursor cells and achieves the effects of high induction efficiency and simple method

Inactive Publication Date: 2009-04-15
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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This induction scheme has

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  • Method for preparing retina neural ganglia progenitor cells
  • Method for preparing retina neural ganglia progenitor cells
  • Method for preparing retina neural ganglia progenitor cells

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Embodiment 1

[0024] Experimental animals used:

[0025] (1) Wild-type C57BL / 6 mice: SPF grade, provided by the Experimental Animal Center of Sun Yat-sen University.

[0026] (2) C57BL / 6-EGFP transgenic mice: strain C57BL / 6-Tg(CAG-EGFP) C14-Y01-FM1310sb, SPF grade, provided by the Institute of Model Animals, Nanjing University.

[0027] (3) Kunming mice: SPF grade, provided by the Experimental Animal Center of Sun Yat-sen University.

[0028] Bone marrow mesenchymal stem cell growth fluid and nerve cell growth fluid:

[0029] Bone marrow mesenchymal stem cell growth medium is used for culturing bone marrow mesenchymal stem cells, and its components include: DMEM / F12 (Gibco), 10% fetal bovine serum by volume, and 1% GlutaMAX (Gibco) by volume. The nerve cell growth solution is used for: the culture of retinal neural precursor cells; the stratification and mixed co-culture of bone marrow mesenchymal stem cells and retinal neural precursor cells. The composition of nerve cell growth medium ...

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Abstract

The invention discloses a method for preparing a precursor cell of a retinal ganglion sample. In the method, the co-culture and/or mixed co-culture of a Transwell layered co-culture system is carried out on bone mesenchymal stem cells and the precursor cell of the retinal ganglion of a rat with gestational age of 13 to 14 days, thereby inducing to prepare the precursor cell of the retinal ganglion sample. The method is simple and easy to be carried out, has high inducing efficiency, does not need to transfer a foreign gene and has the advantages of safety and practicability.

Description

technical field [0001] The present invention relates to cell differentiation, in particular to a method for preparing retinal ganglion-like precursor cells. Background technique [0002] Mammalian retinal ganglion cells are difficult to regenerate after injury. Clinically, a variety of ophthalmic diseases, including glaucoma and ischemic optic neuropathy, can cause retinal ganglion cell damage, eventually leading to irreversible blindness. Research on stem cell replacement therapy has brought new hope for the treatment of such diseases. Retinal neural precursor / progenitor cells (RPCs) belong to immature retinal cells. In vitro and animal experiments have shown that they can integrate and differentiate into mature retinal neurons in the retina after transplanting them into the recipient retina. The visual function of the recipient animals was significantly restored. Studies have found that for a specific type of retinal nerve cells (such as rod cells), the corresponding pre...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/0797
Inventor 葛坚孙雪荣
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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