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Solid phase extraction method based on nylon nano fiber film and extractor

A nanofiber membrane and extraction device technology, which is applied in chemical instruments and methods, ion exchange treatment devices, alkali metal oxides/hydroxides, etc., can solve problems such as cumbersome and time-consuming operations, low extraction efficiency, and unstable extraction rate , to achieve the effect of simple equipment, large extraction capacity and short extraction equilibrium time

Inactive Publication Date: 2010-12-08
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Technical problem: The purpose of this invention is to overcome the shortcomings of existing sample pretreatment methods such as tedious and time-consuming operation, low extraction efficiency or unstable extraction rate, and provide a new and effective solid-phase extraction technology based on nylon nanofiber membranes and corresponding extraction methods. device

Method used

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  • Solid phase extraction method based on nylon nano fiber film and extractor
  • Solid phase extraction method based on nylon nano fiber film and extractor
  • Solid phase extraction method based on nylon nano fiber film and extractor

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preparation example Construction

[0034] The preparation method of the above-mentioned nylon nanofiber membrane is: mixing nylon with a solvent, ultrasonic vibration or magnetic stirring, to obtain an electrospinning solution; controlling the electrospinning conditions and time so that the obtained fiber membrane has a required fiber diameter, Porosity and film thickness: place the nylon nanofiber film in a vacuum drying oven at 60-80°C for 6-24 hours, and remove the residual solvent therein.

[0035] The solvent described in the above preparation method is formic acid, dichloromethane, m-toluene, phenol, calcium chloride-methanol saturated solution, benzyl alcohol, glacial acetic acid, ethylene glycol, etc., and can be a single solvent or a mixed solvent wherein, Or a single or mixed solvent added with a surfactant, a solubilizer or a pH regulator, in which the mass concentration of nylon is 10-70%.

[0036]The adjustable or controllable electrospinning conditions as described above in the preparation method ...

Embodiment 1

[0041] Sample: Environmental water sample Sample volume: 1ml Target substance: Diethylstilbestrol

[0042] Nylon 6 is used as a raw material, formic acid is used as a solvent, and the mass concentration of nylon 6 is 25%, and an electrospinning solution is obtained by magnetic stirring, and a nanofiber film is electrospun into a film thickness of 10 μm. The surface of the fiber is smooth and the diameter is 200-500nm.

[0043] Weigh 1.5 mg of nanofiber membrane and put it into the extraction device, acetonitrile → water → acetonitrile → water (200 μl each) for activation and washing, transfer the sample into the sample tube, centrifuge at 1000 rpm for 3 minutes, the target substance is enriched on the nanofiber membrane, and eluted with 100 μl of acetonitrile The extraction efficiency of diethylstilbestrol enriched on the nanofibers was measured and calculated to be 99.0% (RSD=2.1%, n=6).

Embodiment 2

[0045] Sample: Rabbit plasma Sample volume: 3ml Target substance: Docetaxel

[0046] Nylon 6 was used as a raw material, formic acid was used as a solvent, and the mass concentration of nylon 6 was 44%. The electrospinning solution was obtained by magnetic stirring, and a nanofiber film was electrospun into a film with a thickness of 15 μm. The surface of the fiber is smooth and the diameter is 200-700nm.

[0047] Blood samples were collected, centrifuged to obtain plasma, and diluted 10 times to obtain plasma samples.

[0048] Weigh 1.5mg of nanofiber membrane into the extraction device, activate and wash with acetonitrile→water (200μl each), transfer the sample into the sample tube, centrifuge at 2000rpm for 3min, the target substance is enriched on the nanofiber membrane, and eluted with 200μl of acetonitrile The extraction efficiency of docetaxel on the nanofiber was measured and calculated to be 92.5% (RSD=5.7%, n=6).

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Abstract

The invention provides solid-phase extraction technology based on a nylon nanometer fibrous membrane and an extraction device thereof. The fibrous membrane is prepared by taking nylon as a raw material and adopting the electrostatic spinning method; the fiber is smooth or porous in surface, and the diameter is between 10 and 1,000nm, and the thickness is between 1 and 20mu m; the technology and the device are suitable for the pre-treatment of biological samples such as water samples, soil, blood, animal tissues, plants and the like, and can extract and enrich the target substances in micro and trace quantities. A small connection pipe(2) of the extraction device is connected with a sample pipe(1) and a collection pipe(3), wherein the middle part of the small connection pipe(2) is providedwith a membrane fixed screen, and the nylon nanometer fibrous membrane is arranged and fixed on the screen; and the back end of the sample pipe(1) is closely matched with the front end of the small connection pipe(2), and the back end of the small connection pipe(2) is closely matched with the front end of the collection pipe(3), so that the pipes of the extraction device can be conveniently separated according to operational need. The solid-phase extraction technology and the extraction device thereof related to the invention have the advantages of high efficiency, quickness, stability, the environment friendly realization of the pre-treatment of the samples, easy operation, and simple equipment.

Description

technical field [0001] The design of the invention relates to a solid-phase extraction technology using nylon nanofiber membrane as an extraction medium and a corresponding extraction device, belonging to the field of analysis technology. Background technique [0002] The monitoring of target substances in samples is related to various fields of life and health, such as disease diagnosis, clinical monitoring, drug development, food safety, environmental monitoring, forensic examination, contraband and drug inspection, etc. However, biological samples are complex systems, in which there are many types of coexisting substances, complex shapes, different properties, and very small amounts of target substances. To analyze and detect the composition and content of these trace or even trace target substances, effective separation and enrichment of target substances is a necessary condition for high-sensitivity detection. Statistics show that the sample pretreatment time in the de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/28B01D15/08
Inventor 顾忠泽许茜吴淑燕葛文娜王敏殷雪琰
Owner SOUTHEAST UNIV