Calibration method for temperature test of PCR instrument
A calibration method and technology of a temperature detector, which is applied in the calibration field of PCR instrument temperature test, to achieve the effect of quick and fast calibration
Inactive Publication Date: 2011-04-20
QINGDAO TECHNOLOGICAL UNIVERSITY
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[0003] Aiming at the shortcomings of the manual calibration method, the present invention proposes a calibration method for temperature testing of PCR instruments, which is suitable for data calibration of PCR instruments
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[0040] refer to figure 1 As shown, the hardware structure of the present invention includes a calibration job management server 1 , a temperature detector 2 , a PCR instrument 3 and a printer 4 . The calibration job management server 1 is the core of the calibration system, responsible for scheduling and arranging tasks, issuing orders and transmitting data, and can print out intermediate data during the calibration process through the printer 4 . During the calibration process, according to the calibration process, the calibration operation management server 1 sends calibration instructions, deviation data modification instructions or data to the PCR instrument 3, and at the same time obtains the temperature data detected by the temperature detector 2; the PCR instrument 3 receives the calibration operation management server. After the instruction of 1, start its own calibration program to carry out the calibration operation; the temperature detector 2 obtains the current tem...
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The invention relates to a calibration method for temperature test of a PCR instrument. The calibration method comprises the following steps: selecting N basic temperature key points according to a calibration process and working temperature characteristics of the PCR instrument; sending a calibration instruction, a deviation data modification instruction or other data to the PCR instrument by a calibration operation management server based on the key points; starting self calibration procedure by the PCR instrument for calibration operation after receiving the calibration instruction; and obtaining existing temperature data by a temperature detection instrument through a group of temperature sensing probes fixed on a PCR instrument module and send the temperature data to the calibration operation management server in real time so that the calibration operation management server obtains the temperature data detected by the temperature detection instrument in real time. The calibrationmethod can help quickly and accurately calibrate the temperature data of the PCR instrument, and the calibrated accurate data can be updated in the PCR instrument in real time. The calibration process is automatically completed, which improves calibration efficiency of the PCR instrument, saves calibration time and promotes product competitiveness.
Description
technical field [0001] The invention belongs to a calibration method for a medical temperature control detection system, and in particular relates to a calibration method for temperature testing of a PCR instrument. Background technique [0002] With the rapid development of molecular biology technology and biomedicine, gene detection and diagnostic technology has been widely used in pathogen detection in various fields of clinical medicine, and has become a symbol of a country's advanced diagnostic technology. Polymerase chain reaction (PCR) is a new method for rapid amplification of specific DNA fragments in vitro, which makes it possible to detect specific genes in the amplified products, and has become the mainstream of molecular biology diagnosis in developed countries. The current research focus is mainly on the improvement of polymerase chain reaction PCR technology and the development of automatic detection system for gene amplification. As an important control tool...
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IPC IPC(8): G01K15/00C12Q1/68
Inventor 王金鹤
Owner QINGDAO TECHNOLOGICAL UNIVERSITY