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Medical kit and using method thereof

A kit and culture medium technology, applied in the field of medical kits, can solve the problems of prolonged cell culture time, patients missed timely implantation, etc., to achieve the effect of improving satisfaction, quality and reliability

Inactive Publication Date: 2009-09-09
SEWON CELLONTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, patients may miss out on timely engraftment due to prolonged cell culture time

Method used

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  • Medical kit and using method thereof
  • Medical kit and using method thereof
  • Medical kit and using method thereof

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preparation example Construction

[0043] 4. Kits for the preparation of chondrocyte therapy products (CRM-Product Kit [CRM-Collection Kit, CRM-Delivery Kit, CRM-Media Packaging Kit, CRM-Implantation Kit]) (CRM-Product Kit [CRM-Collection Kit, CRM-Delivery Kit, CRM-MediumPackaging Kit, CRM-Implantation Kit]), which are composed of various sub-kits respectively. The cartilage regeneration kit also includes: a culture medium kit (CRM-RF kit [CSB-I, CSB-II, CSM, CPM, CCM, CCB]) for isolating / cultivating / preparing / cryopreserving cells; and Media kits for isolation / culture / cryopreservation of cells (CRM-FZ kit [Cartisepor, STA, PCF, Pass solution, Cell stock solution, CSM AD solution, CPM AD solution, Neutro solution]), which help For the isolation, culture, collection and implantation of cells.

[0044] In addition, the kit 20 for bone regeneration is a plasticware kit, which includes:

[0045] 1. Bone marrow collection kit (BRM-transport kit) (BRM-Transport Kit);

[0046] 2. Osteoblast Isolation Kit (BRM-Initia...

Embodiment 1

[0065] The cartilage tissue harvesting process involves:

[0066] 1. Open the outer wrapper of the CRM-delivery kit contained in the cartilage RM kit;

[0067] 2. Transfer only the Styrofoam box with the inner packaging to the surgery room leaving the outer packaging removed; and

[0068] 3. Collect cartilage tissue using a sterile hard tissue collector in a styrofoam box, place the collected cartilage tissue in a cartilage tissue delivery container containing red medium, and use a styrofoam box and outer A packaging carton double wraps the cartilage tissue delivery container, and the wrapper is then transferred to the cell culture chamber.

[0069] In addition, the process of isolating chondrocytes of the CRM-Start kit includes:

[0070] 1. Take out the CRM-separation kit from the CRM-starting kit, spray the CRM-separation kit with a sufficient amount of 70% ethanol, and then transfer the CRM-separation kit to the purification bench;

[0071] 2. Weigh the cartilage tissue ...

Embodiment 2

[0183] The bone marrow collection process includes:

[0184] 1. Open the outer wrapper containing the BRM-delivery kit contained in the bone RM kit;

[0185] 2. Transfer only the Styrofoam box with the inner packaging to the surgery room leaving the outer packaging removed; and

[0186] 3. Place the bone marrow in a bone marrow tissue transfer container containing red medium in a Styrofoam box, double pack the bone marrow tissue transfer container with a Styrofoam box and an outer carton, and then place the The packages are transferred to the cell culture chamber.

[0187] In addition, the procedure for isolating osteoblasts of the BRM-starter kit includes:

[0188] 1. Take out the BRM-separation kit from the BRM-starting kit, spray the BRM-separation kit with a sufficient amount of 70% ethanol, and then transfer the BRM-separation kit to the purification bench;

[0189] 2. Weigh the bone marrow tissue delivered by the BRM-delivery kit in a clean bench using a 50ml tube;

...

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Abstract

The present invention provides a medical kit and a method using the same. For this purpose, an aseptic / sterile medical kit is provided which comprises a cartilage regeneration kit 10, a bone regeneration kit 20 or an umbilical cord blood storage kit 30, in a configuration that each process can be performed according to the functionally-specialized kit sets for each step, via division of the overall processes into the corresponding steps for isolation, culture, collection and storage of cells, and implantation of the desired cells into the target sites of the body. Therefore, the cartilage is regenerated by cartilage tissue collection; chondrocyte isolation; chondrocyte medium change and subculture; preparation of a chondrocyte therapy product; media for isolation / culture / preparation / cryopreservation of cells; and media for isolation / culture / cryopreservation of cells, using the cartilage regeneration kit 10. The bone is regenerated by bone marrow collection; osteoblast isolation; osteoblast medium change and subculture; and preparation of an osteoblast therapy product, using the bone regeneration kit 20. In addition, the umbilical cord blood is stored by umbilical cord blood collection; hematopoietic stem cell isolation; and cryopreservation of hematopoietic stem cells, using the umbilical cord blood storage kit 30.

Description

technical field [0001] The present invention relates to medical kits and methods of using the kits. More specifically, the present invention relates to methods using a kit related to the preparation and implantation of cell therapy products for the regeneration of cartilage and bone, as well as the storage and processing of cord blood. In particular, the present invention enables the preparation and provision of various materials, media and solutions necessary for the isolation, culture, collection, implantation and storage processes of desired cells in a single kit form for easy and convenient use. Therefore, the present invention can solve some problems existing in conventional techniques of preparing cell therapy products and storing umbilical cord blood, such as high production cost, long production cycle and high labor cost. Therefore, the present invention can significantly improve the quality and reliability of products, thereby greatly contributing to the improvement ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28
CPCA61F2/30756A61L27/3852A61F2/28A61L27/3834A61L27/3821A61L27/3817A61L27/3847A61P7/00A61P19/00A61P43/00
Inventor 孙炫美张晶皓张在德
Owner SEWON CELLONTECH CO LTD
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