Reagent card for detecting human ABO/RHD blood-grouping and detection method using same

Abstract

The invention discloses a reagent card for detecting human ABO / RHD blood-grouping and a detection method using the same. The reagent card comprises a lower casing and an upper casing, wherein, three parallel grooves are formed in the lower casing; three substrates are arranged in the three grooves respectively; mats and reaction films are stuck on the three substrates; one end of the mat is in mutual lap joint with the reaction film; an anti-A detection line and a control line are arranged on the first reaction film; an anti-B detection line and a control line are arranged on the second reaction film; an anti-RHD detection line and a control line are arranged on the third reaction film; three liquid-dripping openings and three blood-dripping openings corresponding to the three mats are sequentially arranged on the upper casing; three inspection windows corresponding to the three detection lines and the three control lines are formed on the upper casing; and the blood-dripping openings are positioned between the liquid-dripping openings and the inspection windows. The detection method comprises the following steps: blood-dripping, liquid-dripping, erythrocyte migration and chromatography and result observation, therefore, the invention has the advantages of shorter detection time, higher detection accuracy, lower cost and more convenient detection and observation.

Description

Technical field: [0001] The invention relates to a reagent card for detecting blood typing and a detection method thereof, in particular to a reagent card for detecting human ABO and RHD blood typing and a detection method thereof, which is suitable for hospitals and families. Background technique: [0002] The red blood cell blood type is divided according to the red blood cell surface antigen in the blood. According to the different types of antigens, there are more than 20 common classification systems, and the ABO blood group system is just one of them. The principle of ABO blood type classification is: there are no A and B blood group antigens on red blood cells, but there are anti-A and anti-B antibodies in the serum, which is called O type; there are A antigens on red blood cells, but there are anti-B antibodies in the serum, called A Those with B antigens on red blood cells and anti-A antibodies in their serum are called type B; those with A and B antigens on red blo...

AI Technical Summary

Problems solved by technology

[0003] At present, the reagents mainly used for ABO and RHD blood typing mainly adopt the agglutination method and the micro-column gel method. The agglutination kit using the agglutination method does not have a contrast, the result judgment cannot be standardized, and the sensitivity is poor, and the D blood type cannot be detected; the micro-column agglutination The gel method requires a special gel card, the cost of the gel is relatively high, the card needs a special mold, and a special centrifuge is equipped to make the method take 30-40 minutes to detect blood type, and the cost of the experiment is relatively high. Currently, there is another test method, that is, there are anti-A detection lines, anti-B detection lines, anti-RHD detection lines and quality control lines on the reaction layer. If the blood is AB type, most of the red blood cells in the blood will be absorbed by the anti-A detection line, and the anti-B The test line is slightly red, which means that the tester is suspicious of the test result and the test result is not accurate

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