Graft inoculation identification method of tomato yellow leaf curl virus disease
A technology of tomato yellow leaf curl and yellow leaf curl virus, which is applied in the field of inoculation method of tomato yellow leaf curl virus disease, can solve problems such as difficult identification of whitefly inoculation and inability to use whitefly inoculation identification, etc. , to achieve the effect of simple vaccination identification method and guaranteed accuracy
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Tomato varieties were selected from 2300.
[0020] (1) select the tomato that has been infected with yellow leaf curl virus disease as stock, carry out the seedling raising of the material to be identified, when two true leaves, adopt oblique cutting method to graft it on the susceptible stock, the first 3 after grafting Day, keep 90% humidity, temperature is 20 ℃, shading grows seedlings, after grafting 5 days, in 50% humidity, temperature is the normal cultivation under the condition of 25 ℃;
[0021] During the nursery of the material to be identified, use Actay to prevent the infestation of Bemisia tabaci;
[0022] (2) 15 days after grafting, 30 days, extract the DNA of scion respectively, whether contain tomato yellow leaf curl virus disease in scion with PCR method detection;
[0023] The specific steps are as follows:
[0024] Scion DNA extraction method:
[0025] 1. Add 2 pieces of fresh leaves the size of the cap of the centrifuge tube to the 1.5ml centrifug...
Embodiment 2
[0050] Tomato varieties were selected from 2583.
[0051] 1) Select the tomato infected with yellow leaf curl virus as the rootstock, raise the seedlings of the material to be identified, and when there are two true leaves, use the oblique cutting method to graft it on the susceptible rootstock, and the first 5 days after grafting , maintain 95% humidity, temperature is 25 ℃, shading seedling cultivation, after grafting 5 days, in 70% humidity, the temperature is normal cultivation under the condition of 30 ℃;
[0052] During the nursery of the material to be identified, use Actay to prevent the infestation of Bemisia tabaci;
[0053] (2) 15 days after grafting, 30 days, extract the DNA of scion respectively, whether contain tomato yellow leaf curl virus disease in scion with PCR method detection;
[0054] The specific steps are as follows:
[0055] Scion DNA extraction method:
[0056]1. Add 1 to 2 pieces of fresh leaves the size of the cap of the centrifuge tube into a 1....
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More