Egg yolk culture medium for amino acid neutralization
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An amino acid and medium technology, applied in the field of neutralizing amino acid yolk medium, can solve problems such as unreliable results and design errors, and achieve the effect of sensitive method, high accuracy and high sensitivity
Inactive Publication Date: 2009-10-28
曲奕
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Therefore, experiments that do not use measures to remove residual antibiotics should be co...
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[0012] Below in conjunction with embodiment the present invention is described in further detail:
[0013] Configure according to the data listed in the table below (grams, ml / liter of distilled water) and the steps described:
[0014] Recipe one:
[0015] Amino acid growth-promoting liquid (containing 8 kinds of amino acids such as cystamine, glutamic acid, arginine, methionine, proline, aspartic acid, serine, isoleucine, cystine and cysteine, hypoxanthine, Uracil 2 bases,) 2ml; polyvalent peptone 9.0-12.0; peptone 4.0-5.0; tryptone 4.0-5.0; yeast extract powder 4.0-6.0; glucose 1.0-2.0; soluble starch 1.5-2.5; Sodium chloride 14.0-16.0; dipotassium hydrogen phosphate 3.0-5.0; potassium dihydrogen phosphate 1.5-2.5; agar powder 14.0-16.0; 2.5mg / L polymyxin B 0.2-0.3ml; 3mg / L vancomycin 0.2 -0.3ml; 15% sterile egg yolk saline 10.0-15.0ml; soil temperature -800.3-0.5; lecithin 0.5-0.8; double distilled water 1000ml, pH7.4.
[0016] ...
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Abstract
The invention relates to an egg yolk culture medium for amino acid neutralization, in particular to a prescription of a culture medium used when a gonorrhea patient need the gonococcus culture examination after using a large amount of clinical antibiotics and a preparation method of the culture medium. The gonococcus culture examine is an important method used for diagnosing gonorrhea. The purulent inflammation which is the disease of genitourinary system and caused by gonococcus is one of the pathogens to which clinical doctors attach importance. Especially when the large amount of clinical antibiotics is used, the gonococcus detection is very difficult because a sample contains a large amount of residual antibiotics, and the recovered gonococcus culture is still influenced by the residual antibiotics, therefore the design mistake of not adopting an experiment for removing the residual antibiotics is very serious, and the result obtained by the experiment is not reliable. The invention aims to provides an egg yolk culture medium for amino acid neutralization, which is used for clinically judging the gonococcus used in the sample of a genitourinary tract.
Description
technical field [0001] The invention relates to a neutralizing amino acid egg yolk culture medium, especially the formula and preparation method of the culture medium for gonorrhea patients who need gonococcal culture and inspection after a large amount of clinical antibiotics are used. The main ingredients of its formula are: amino acid growth-promoting liquid (containing 8 kinds of cystamine, glutamic acid, arginine, methionine, proline, aspartic acid, serine, isoleucine, cystine and cysteine) Amino acid, hypoxanthine, uracil two kinds of bases), polyvalent peptone, peptone, tryptone, yeast extract powder, glucose, soluble starch, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, Agar powder, polymyxin B, vancomycin, egg yolk, Tween-80, lecithin. Gonorrhea culture examination is an important method for the diagnosis of gonorrhea. Especially for chronic patients and female patients, it is necessary to make a correct diagnosis after being cultu...
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