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Method for establishing high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves

The invention is a technology of high frequency regeneration of C. japonica, which is applied in the fields of plant regeneration, horticultural methods, botanical equipment and methods, etc. It can solve the problems of high survival rate, low seed germination rate, short proliferation time, etc., and achieve robust growth. , Significant economic benefits, good growth effect

Inactive Publication Date: 2009-12-09
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a tissue culture method for the establishment of a high-frequency regeneration system and rapid propagation of the leaves of S. sylvestris s. The problem of tissue culture seedlings, the buds cultivated by this method are healthy, strong, and the proliferation time is short, and the survival rate of mature seedlings after transplantation is high

Method used

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  • Method for establishing high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves
  • Method for establishing high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves
  • Method for establishing high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves

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Embodiment 1

[0023] Material collection method: select some young leaves removed during subculture transfer by stem segment propagation or seed propagation and directly inoculate as explants;

[0024] Medium:

[0025] The MS basic medium was prepared using the formula in "Tissue Culture and Application of Woody Plants" published by Beijing Higher Education Press and edited by Chen Zhenghua in 1986.

[0026] The preparation of the medium: the basic medium is WPM, the growth hormone is ZE, NAA, IBA, the medium is 20g / L of Su (sucrose), Ag + 7.5g / L, PH value is 5.8, and the additives are activated carbon Ac, etc.; the thickness of the medium is generally 1.4~1.6cm;

[0027] Bud induction medium→WPM+ZE(1.0mg / L)+NAA(1.0mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0028] Cluster bud induction medium→WPM+ZE(1.0mg / L)+NAA(0.5mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0029] Bud proliferation medium → WPM+ZE (2.0mg / L -1 )+NAA(0.3mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0030] Rooting m...

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Abstract

The invention provides a method for establishing a high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves, and belongs to the technique of culturing euscaphis konishii hayata embryo. The method solves the problems existing in the prior art that the seed germination rate of the euscaphis konishii hayata embryo is low, and well purified tissue culture seedlings cannot be provided in large amount. In the method, partial tender leaves removed during the sub-transplanting in the stem reproduction or seed reproduction are selected as materials, and the tender leaves undergo induced culture for 15 to 20 days to grow strong seedlings; after proliferation culture for 20 to 30 days and induced rooting for 20 to 25 days, the seedlings are grown and transplanted, and the survival rate is as high as more than 95 percent; and the propagation factor is higher, and the survived seedlings are tall and strong, and well grown.

Description

technical field [0001] The invention belongs to the cultivation technique of the embryo of Crow's Crow, and more specifically relates to a method for establishing a high-frequency regeneration system and rapid propagation of Crow's crow leaf. Background technique [0002] Corvus chinensis belongs to the genus Corvus genus of the family Xinggu Oilaceae, and is also known as Huabromu, Scalewood, Pascal, and Kaijiao. It is a new species of Fujian Corvus discovered by Professor Xu Bingsheng of the Biology Department of Xiamen University in the 1960s ( Euscaphis fukienensis Hsu), later also referred to as this species, is a unique ornamental tree species in my country. It blooms in summer, bears fruit in winter, and has a long fruit period. When the red fruit matures, it cracks along the ventral suture. Together with its black seeds, it looks like a beautiful and lifelike red butterfly. It has great ornamental value and can be used as a landscape tree or street tree. The fruit-ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 何碧珠邹双全李玉平
Owner FUJIAN AGRI & FORESTRY UNIV
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