Method for establishing high-frequency regeneration system of euscaphis konishii hayata leaves and quickly reproducing euscaphis konishii hayata leaves
The invention is a technology of high frequency regeneration of C. japonica, which is applied in the fields of plant regeneration, horticultural methods, botanical equipment and methods, etc. It can solve the problems of high survival rate, low seed germination rate, short proliferation time, etc., and achieve robust growth. , Significant economic benefits, good growth effect
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[0023] Material collection method: select some young leaves removed during subculture transfer by stem segment propagation or seed propagation and directly inoculate as explants;
[0024] Medium:
[0025] The MS basic medium was prepared using the formula in "Tissue Culture and Application of Woody Plants" published by Beijing Higher Education Press and edited by Chen Zhenghua in 1986.
[0026] The preparation of the medium: the basic medium is WPM, the growth hormone is ZE, NAA, IBA, the medium is 20g / L of Su (sucrose), Ag + 7.5g / L, PH value is 5.8, and the additives are activated carbon Ac, etc.; the thickness of the medium is generally 1.4~1.6cm;
[0027] Bud induction medium→WPM+ZE(1.0mg / L)+NAA(1.0mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)
[0028] Cluster bud induction medium→WPM+ZE(1.0mg / L)+NAA(0.5mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)
[0029] Bud proliferation medium → WPM+ZE (2.0mg / L -1 )+NAA(0.3mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)
[0030] Rooting m...
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