Aurosol test paper used for quickly detecting betamethasone residue and preparation method thereof
A technology of colloidal gold test paper and betamethasone, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of human health hazards, complicated processing procedures, glucocorticoid residues, etc., and achieve less time-consuming and simple pre-treatment process Effect
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[0031] (1) Use of test strips: Dilute the sample extract in the box to 5ml for later use. Take 1.0 g of the test sample, add 2 ml of extract to grind and extract, take 50 μl of the supernatant, mix it with loading buffer, add it dropwise to the sample pad, and place it at room temperature for 10 minutes before reading.
[0032] (2) If negative, the betamethasone content in the sample is 8ng / mL
[0033] 1. Coupling of betamethasone with carrier protein:
[0034] Using the mixed acid anhydride method, on the side chain of the D ring, on the C21 position of the molecule -OH, succinylated, activated the terminal carboxyl group of succinic acid, linked with -NH2 in the BSA or OVA molecule, and synthesized the betamethasone immunogen and the coating original. A 20% ethanol solution with a concentration of 50 μg / mL of the original drug was prepared, and the maximum absorption wavelength was known by ultraviolet scanning. Prepare an aqueous solution of 200 μg / mL bovine serum albumin...
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