Aurosol test paper used for quickly detecting betamethasone residue and preparation method thereof

A technology of colloidal gold test paper and betamethasone, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of human health hazards, complicated processing procedures, glucocorticoid residues, etc., and achieve less time-consuming and simple pre-treatment process Effect

Inactive Publication Date: 2009-12-23
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

The unreasonable use of glucocorticoids as growth-promoting agents, or the use of veterinary drugs without paying attention to the withdrawal period may eventually lead to the residue of glucocorticoids in animal-derived foods, which constitutes a potential hazard to human health.
[0003] The conventional analysis method of the glucocorticoid betamethasone residue is mainly an instrument method such as liquid chromatography. This traditional analysis method not only requires an expensive gas chromatograph, but also has slo

Method used

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  • Aurosol test paper used for quickly detecting betamethasone residue and preparation method thereof
  • Aurosol test paper used for quickly detecting betamethasone residue and preparation method thereof
  • Aurosol test paper used for quickly detecting betamethasone residue and preparation method thereof

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Embodiment Construction

[0031] (1) Use of test strips: Dilute the sample extract in the box to 5ml for later use. Take 1.0 g of the test sample, add 2 ml of extract to grind and extract, take 50 μl of the supernatant, mix it with loading buffer, add it dropwise to the sample pad, and place it at room temperature for 10 minutes before reading.

[0032] (2) If negative, the betamethasone content in the sample is 8ng / mL

[0033] 1. Coupling of betamethasone with carrier protein:

[0034] Using the mixed acid anhydride method, on the side chain of the D ring, on the C21 position of the molecule -OH, succinylated, activated the terminal carboxyl group of succinic acid, linked with -NH2 in the BSA or OVA molecule, and synthesized the betamethasone immunogen and the coating original. A 20% ethanol solution with a concentration of 50 μg / mL of the original drug was prepared, and the maximum absorption wavelength was known by ultraviolet scanning. Prepare an aqueous solution of 200 μg / mL bovine serum albumin...

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Abstract

The invention discloses an aurosol test paper used for quickly detecting betamethasone residue and a preparation method thereof. The test paper includes a box body, a test paper strip, a sponge holder and a holder, wherein the test paper strip absorbs envelope antigen and betamethasone polyclonal antibody labeled by the aurosol. The invention overcomes the defects of a betamethasone physical and chemical analysis method such as complex operation, high detection cost, slow speed and secondary environment pollution, and can accurately and sensitively detect the betamethasone residue in water, meat products and milk products. Besides, with little time consumed in the simple pretreatment process of samples, the invention can simultaneously and quickly detect a great quantity of samples.

Description

technical field [0001] The invention relates to a colloidal gold test paper for rapid detection of betamethasone residues and a preparation method thereof. Background technique [0002] Betamethasone, a veterinary drug, is a glucocorticoid drug that has good anti-inflammatory effects and can fight against inflammation caused by various reasons such as physical, chemical, physiological, immune, etc. In addition, it can also improve the feed conversion rate, thereby increasing animal body weight, and has a synergistic effect with β-agonists and other steroid hormones. The unreasonable use of glucocorticoids as growth-promoting agents, or careless withdrawal periods when used as veterinary drugs may eventually lead to the residue of glucocorticoids in animal-derived foods, which constitutes a potential hazard to human health. [0003] The conventional analysis method of the glucocorticoid betamethasone residue is mainly an instrument method such as liquid chromatography. This ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/544
Inventor 曹远银孙艳秋王菡储晓刚雍炜凌云
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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