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Method for quickly detecting content of L-glutamine in fermentation liquor

A technology of glutamine and fermented liquid, applied in the field of amino acid fermentation, can solve the problems of imprecise methods, complicated operation, high cost, etc., and achieve the effects of good timeliness, easy operation, and low cost

Active Publication Date: 2015-03-11
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for rapidly detecting the content of L-glutamine in the fermentation broth in view of technical problems such as imprecise, complicated operation and high cost in the prior art for detecting the content of L-glutamine in the fermentation broth , the method can quickly detect the L-glutamine content in the fermentation broth, with high accuracy, fast and simple, low cost and good timeliness

Method used

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Examples

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Embodiment 1

[0027] A method for rapidly detecting L-glutamine content in fermented liquid, comprising the following steps:

[0028] Step 1, take the production strain of glutamine, insert it into the seed tank after activation for cultivation, then transplant it into the fermentation medium with 10% inoculation amount for cultivation, and obtain the fermentation liquid for subsequent use; in the fermentation medium , according to the content of nutrients below the mass percentage, they are glucose 7%, corn steep liquor 1%, NH 4 Cl 2%, KH 2 PO 4 0.2%, MgSO 4 0.08%, KCl 0.1%; calculated per liter, the addition amount of the following nutrients are yeast powder 1.5g / L, FeSO 410mg / L, CuSO 4 2mg / L, thiamine 1mg / L; Also be added with the inhibitor citric acid that mass percent is 0.1% in the described fermentation medium, and by MnCl 2 and ZnSO 4 The activator of glutamine synthetase of composition, and the addition amount is respectively MnCl 2 50mg / L and ZnSO 4 80mg / L;

[002...

Embodiment 2

[0036] A method for rapidly detecting L-glutamine content in fermented liquid, comprising the following steps:

[0037] Step 1, take the production strain of glutamine, insert it into a seed tank for cultivation after activation, then transplant it into a fermentation medium with a 10% inoculum size for cultivation, and obtain a fermentation liquid for subsequent use; in the fermentation medium , according to the content of nutrients below the mass percentage, they are glucose 3%, corn steep liquor 3%, NH 4 Cl 4%, KH 2 PO 4 0.4%, MgSO 4 0.2%, KCl0.2%; calculated per liter, the added amount of the following nutrients are yeast powder 1g / L, FeSO 4 20mg / L, CuSO 4 10mg / L, thiamine 10mg / L; Also be added with the inhibitor citric acid that mass percent is 0.2% in the described fermentation medium, and by MnCl 2 and ZnSO 4 The activator of glutamine synthetase of composition, and the addition amount is respectively MnCl 2 150mg / L and ZnSO 4 10mg / L;

[0038] Step 2. Tak...

Embodiment 3

[0045] A method for rapidly detecting L-glutamine content in fermented liquid, comprising the following steps:

[0046] Step 1, take the production strain of glutamine, insert it into a seed tank for cultivation after activation, then transplant it into a fermentation medium with a 10% inoculum size for cultivation, and obtain a fermentation liquid for subsequent use; in the fermentation medium , according to the content of nutrients below the mass percentage, they are glucose 5%, corn steep liquor 2%, NH 4 Cl 3%, KH 2 PO 4 0.3%, MgSO 4 0.1%, KCl 0.15%; calculated per liter, the added amount of the following nutrients are yeast powder 1.2g / L, FeSO 4 15mg / L, CuSO 4 6mg / L, thiamine 6mg / L; Also add the inhibitor citric acid that mass percent is 0.15% in the described fermentation medium, and by MnCl 2 and ZnSO 4 The activator of glutamine synthetase of composition, and the addition amount is respectively MnCl 2 100mg / L and ZnSO 4 45mg / L;

[0047] Step 2: Take the f...

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Abstract

The invention discloses a method for quickly detecting the content of L-glutamine in a fermentation liquor. The method comprises the following steps: (1) selecting a cultivation strain of glutamine for activating culture, and then transplanting the cultured strain to a fermentation medium for cultivation; (2) conducting oscillation and centrifugation on the fermentation liquor to obtain a liquid to be detected; (3) preparing a developing solvent, a color developing agent and an eluant; (4) drawing a standard curve; (5) utilizing the liquid to be detected obtained in step (2) for sample spotting on a thin silica gel plate, carrying out developing, color developing, elution and sampling, utilizing an ultraviolet-visible spectrophotometer to measure a light absorption value, and obtaining the value, corresponding to the light absorption value, on the standard curve drawn in step (4), wherein the value is the content of the L-glutamine in the fermentation liquor. The method can be used for detecting the content of the L-glutamine in the fermentation liquor quickly, and is high in accuracy, fast, simple, low in cost and high in timeliness.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a method for rapidly detecting the content of L-glutamine in a fermentation liquid. Background technique [0002] L-Glutamine is a non-essential amino acid for humans and animals, but it has many unique physiological functions, such as improving human immunity and significantly promoting brain function and gastrointestinal function. At present, it has a wide range of applications and good development prospects in the fields of food health care, feed additives, medicine and health. At present, L-glutamine is mainly produced by fermentation at home and abroad. The more commonly used detection methods for glutamine in fermentation liquid mainly include automatic amino acid analyzer method, acid hydrolysis method for ammonia measurement, high performance liquid chromatography, paper chromatography, etc. . The automatic amino acid analyzer method has po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 岳贵龙孙朝阳徐晓鹏
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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