Kit for quantitatively detecting BCR/ABL mRNA level
A quantitative detection and kit technology, which is applied in the field of kits for quantitative detection of BCR/ABL mRNA levels, can solve the problems of poor prognosis and achieve the effects of cost reduction, easy use and strong practicability
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[0025] Embodiment 1, the preparation of the kit of quantitative detection BCR / ABL mRNA level
[0026] 1. Design of primers and probes in the kit for quantitative detection of BCR / ABL mRNA levels
[0027]The composition of the M-type BCR / ABL real-time quantitative PCR system is as follows:
[0028] (1) Upstream primer (located at BCR exon 13): 5'-CCGCTGACCATCAATAAGGAA-3' (sequence 1 in the sequence listing), with a final concentration of 0.3 μM;
[0029] (2) Downstream primer (located at ABL exon 2): 5'-CTCAGACCCTGAGGCTCAAAGT-3' (sequence 2 in the sequence listing), with a final concentration of 0.3 μM;
[0030] (3) TaqMan probe (located in ABL exon 2): 5'-AGCCCTTCAGCGGCCAGTAGCATCT-3' (sequence 3 in the sequence listing), the final concentration is 0.2 μM;
[0031] (4) Master Mix for fluorescent PCR (purchased from ABI, USA).
[0032] The composition of the m-type BCR / ABL real-time quantitative PCR system is as follows:
[0033] (1) Upstream primer (located at BCR exon 1): ...
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