Rare earth-chitosan oligosaccharide complex and preparation method and application thereof

A technology for chitosan oligosaccharide and complexes, which is applied to rare earth-chitosan oligosaccharide complexes and the fields of preparation and application thereof, can solve the problems of low bioavailability, many adverse reactions, high toxicity, etc., and achieves high bioavailability, human body and environmentally friendly, easy-to-absorb effects

Inactive Publication Date: 2010-01-27
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the technical problems to be solved by the present invention is to provide a rare earth-chitooligosaccharide complex with synergistic anticancer activity of rare earth ions and chitooligosaccharides, so as to overcome the high toxicity, many adverse reactions, The defect of low bioavailability adds a new category to existing anticancer drugs

Method used

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  • Rare earth-chitosan oligosaccharide complex and preparation method and application thereof
  • Rare earth-chitosan oligosaccharide complex and preparation method and application thereof
  • Rare earth-chitosan oligosaccharide complex and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] a) Take by weighing 2.00mmol of oligochitosan with a molecular weight of 3000, add 40ml of deionized water to prepare an aqueous solution of oligochitosan with a concentration of 0.05mol / L;

[0034] b) Weigh 0.50 mmol of ammonium cerium nitrate, add 10 ml of deionized water, and prepare an aqueous solution of ammonium cerium nitrate with a concentration of 0.05 mol / L;

[0035] c) at room temperature, drop the prepared cerium ammonium nitrate aqueous solution into the prepared chitosan oligosaccharide aqueous solution, and the dropping rate is controlled at 40-60 drops / minute;

[0036] d) adjusting the pH value of the reaction system to 5 with sodium hydroxide;

[0037] e) react at 25°C for 5 hours, concentrate, add absolute ethanol to crystallize;

[0038] f) Suction filtration, washing the filter residue with deionized water for 3 to 4 times, and vacuum drying at 45° C. for 5 to 6 hours to obtain: cerium (IV)-chitooligosaccharide complex.

[0039] figure 1 For the s...

Embodiment 2

[0044] a) Take by weighing 2.00mmol of oligochitosan with a molecular weight of 5000, add 40ml of deionized water to prepare an aqueous solution of oligochitosan with a concentration of 0.05mol / L;

[0045] b) Weigh 0.50 mmol of ammonium cerium nitrate, add 10 ml of deionized water, and prepare an aqueous solution of ammonium cerium nitrate with a concentration of 0.05 mol / L;

[0046] c) at room temperature, drop the prepared cerium ammonium nitrate aqueous solution into the prepared chitosan oligosaccharide aqueous solution, and the dropping rate is controlled at 60-80 drops / min;

[0047] d) adjusting the pH value of the reaction system to 5 with sodium hydroxide;

[0048] e) react at 25°C for 5 hours, concentrate, add absolute ethanol to crystallize;

[0049] f) Suction filtration, washing the filter residue with deionized water for 3 to 4 times, and vacuum drying at 50° C. for 4 to 5 hours to obtain: cerium (IV)-chitooligosaccharide complex.

[0050] After analysis, it is ...

Embodiment 3

[0052] a) Take by weighing 2.00mmol of oligochitosan with a molecular weight of 10000, add 40ml of deionized water, and prepare an aqueous solution of oligochitosan with a concentration of 0.05mol / L;

[0053] b) Weigh 0.50 mmol of ammonium cerium nitrate, add 10 ml of deionized water, and prepare an aqueous solution of ammonium cerium nitrate with a concentration of 0.05 mol / L;

[0054] c) at room temperature, drop the prepared cerium ammonium nitrate aqueous solution into the prepared chitosan oligosaccharide aqueous solution, and the dropping rate is controlled at 60-80 drops / minute;

[0055] d) adjusting the pH value of the reaction system to 5 with sodium hydroxide;

[0056] e) react at 25°C for 5 hours, concentrate, add absolute ethanol to crystallize;

[0057] f) Suction filtration, washing the filter residue with deionized water for 3 to 4 times, and vacuum drying at 50° C. for 4 to 5 hours to obtain: cerium (IV)-chitooligosaccharide complex.

[0058] After analysis, ...

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Abstract

The invention discloses a rare earth-chitosan oligosaccharide complex, in particular to a binary complex formed by the coordination of rare earth ions and chitosan oligosaccharide; the complex is nanoparticles, the average particle size of the particles is 50-100nm, and the particles are distributed evenly. The final concentration of the complex for prompting the apoptosis of K562 cancer cells is 0.001mu g / mL-10mu g / mL, the inhibition ratio of 1mu g / mL of the complex to K562 cancer cells can reach 47% and the complex can be used to prepare the anti-K562 cancer cell drug. Compared with the prior art, the rare earth-chitosan oligosaccharide complex has good water-solubility, low toxicity and high bioavailability, the antitumor activity of the complex is better than any single ligand, the complex has synergetic effect, good chemical stability and good thermal stability, the complex can be used, stored and transported at room temperature; in addition, the preparation method of the invention has the advantage of simple operation, wide raw material sources, green and environmentally friend and the like.

Description

technical field [0001] The invention relates to a rare earth-chitooligosaccharide complex and its preparation method and application, in particular to a binary complex formed by rare earth ions and chitooligosaccharide with a coordination bond, its preparation method and its Application in anticancer drugs. Background technique [0002] Cancer is one of the important diseases that seriously threaten human health at present. The current treatment methods include surgical resection, radiotherapy, chemical drug therapy, etc., but still mainly rely on chemical drug therapy. Currently, there are many kinds of chemical drugs used clinically to treat cancer, such as platinum, nitrogen mustards, triazoles, etc., but the application of most drugs is limited due to high toxicity, many adverse reactions, and low bioavailability. Therefore, searching for highly effective and low-toxic anticancer drugs has become one of the key research topics in the field of medicinal chemistry. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K31/722A61K33/24
Inventor 何其庄杨自芳许东芳沈敬一沈智慧施阳
Owner SHANGHAI NORMAL UNIVERSITY
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