Phase-change monocyte separation medium

A technology for monocytes and separation liquid, which is applied in microorganisms, biochemical equipment and methods, tissue culture, etc., and can solve the problems of high operating proficiency requirements, unfavorable batch tests, and high reagent consumption.

Inactive Publication Date: 2010-03-03
CHENGDU RICH SCI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] This method requires high proficiency in operation and consumes a lot of reagents, which is not conducive to batch testing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Provides a separation medium for the isolation of mononuclear cells from peripheral blood, and provides the required mononuclear cell suspension for flow cytometry detection. The components of the separation liquid are dextran (54% by mass percentage concentration), diatrizoate meglumine (37% by mass percentage concentration), fluorocarbon surfactant and polyvinylpyrrolidone. 2.2 parts of dextran, 0.8 part of diatrizoate meglumine, 0.012 parts of fluorocarbon surfactants, and 0.08 parts of polyvinylpyrrolidone are mixed in a container by mass ratio, and the final density (relative density referred to herein, is The density of pure water under certain temperature conditions is regarded as 1, and the density of another substance under the same conditions is divided by it, the same below) d=1.078 (under 20°C). The density of PBMC is different from other components in the blood and the density of the separation fluid. The density of red blood cells is 1.092, and the density...

Embodiment 2

[0027] Provides a separation medium for the isolation of mononuclear cells from peripheral blood, and provides the required mononuclear cell suspension for flow cytometry detection. The components of the separating liquid are dextran (66% by mass percentage concentration), diatrizoate (30% by mass percentage concentration), fluorocarbon surfactant and polyvinylpyrrolidone. 1.8 parts of dextran, 1.2 parts of diatrizoate meglumine, 0.008 parts of fluorocarbon surfactant, and 0.12 parts of polyvinylpyrrolidone are mixed in a container, and the final density (relative density referred to herein is the The density of pure water under certain temperature conditions is regarded as 1, and the density of another substance under the same conditions is divided by it, the same below) d=1.076 (under the condition of 20°C). The density of PBMC is different from other components in the blood and the density of the separation fluid. The density of red blood cells is 1.092, and the density of ...

Embodiment 3

[0030] Provides a separation medium for the isolation of mononuclear cells from peripheral blood, and provides the required mononuclear cell suspension for flow cytometry detection. The components of the separating liquid are dextran (60% by mass percentage concentration), diatrizoate (34% by mass percentage concentration), fluorocarbon surfactant and polyvinylpyrrolidone. 2 parts of dextran, 1 part of diatrizoate meglumine, 0.01 part of fluorocarbon surfactant, and 0.1 part of polyvinylpyrrolidone are mixed in a container by mass, and the final density (relative density referred to herein, is The density of pure water under certain temperature conditions is regarded as 1, and the density of another substance under the same conditions is divided by it, the same below) d=1.077 (under 20°C). The density of PBMC is different from other components in the blood and the density of the separation fluid. The density of red blood cells is 1.092, and the density of PBMC is 1.07. Accordi...

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PUM

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Abstract

The invention provides a separation medium for separating monocytes in peripheral blood, mainly aiming at providing a monocyte suspension for a flow cytometry detection method. The separation medium comprises 1.8-2.2 parts by mass of glucan, 0.8-1.2 parts by mass of urografin, 0.008-0.012 part by mass of fluorocarbon surfactant and 0.08-0.12 part by mass of polyvinylpyrrolidone. The separation medium has the advantages that the separation medium is in stagnant glue state when standing still, and does not enter the blood after blood collection so as to facilitate sample conveyance; during centrifuging, the separation medium is liquefied and flows, thereby simply and effectively separating the monocytes from the blood; and the separation medium is especially suitable for batch sample processing.

Description

Technical field: [0001] The invention provides a separating liquid for separating mononuclear cells in peripheral blood, and provides mononuclear cell suspension for flow cytometry. Background technique: [0002] Immune cells refer to cells that participate in or are related to the immune response, mainly including lymphocytes, monocyte-macrophages, dendritic cells, various granulocytes, and mast cells. Peripheral blood mononuclear cell (PBMC) mainly refers to lymphocytes, which play a central role in the body's immune response process, accounting for 20%-40% of the total peripheral blood leukocytes, and are the most commonly used in immunological experiments. cell population. Lymphocytes are a heterogeneous cell population that includes many subpopulations with different immune functions. The determination of lymphocyte subsets is mainly used to understand whether the immune function of the patient's body is in a balanced state, thereby differentially diagnosing leukemia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08
Inventor 王军
Owner CHENGDU RICH SCI IND
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