Anti-PCVD shRNA and design-synthesis method and application thereof

A technology of porcine circovirus and virus, applied in antiviral agents, DNA/RNA fragments, DNA preparation, etc.
CN101659953AInactive Publication Date: 2010-03-03ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2010-03-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for designing and synthesizing anti-PCV shRNA. The method comprises the steps of: 1) selecting a section of nucleotide sequence with length of 19bp according to genomesequence of PCV-2 and by taking Rep gene or Cap gene as target gene; 2) selecting a nucleotide sequence as a candidate nucleotide sequence, which meets the following three conditions: GC content of the nucleotide sequence is between 40 percent and 60 percent; at least three A or T nucleic-acid residues exist among locus from 15 to 10; and secondary structure is ensured not to be generated by 19bpnucleotide sequence after inspection; and 3) comparing the obtained candidate nucleotide sequence with PCV genome so as to determine whether the candidate nucleotide sequence only specially aims at the target gene. The anti-PCV shRNA provided by the invention can play the anti-virus role by restricting reproduction of PCV in cells.
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Description

technical field

[0001] The invention relates to the key technology and method of using RNA interference technology to resist porcine circovirus disease. Background technique

[0002] RNA interference (RNA interference, RNAi) refers to the phenomenon of efficient and specific degradation of homologous mRNA induced by double-stranded RNA (double-stranded RNA, dsRNA), which is highly conserved during evolution. Since the use of RNAi technology can specifically knock out or shut down the expression of specific genes, this technology has been widely used in the field of gene therapy for exploring gene functions and infectious diseases and malignant tumors.

[0003] RNAi was first discovered in Caenorhabditis elegans (C.elegans) by Fire et al. They found that injecting dsRNA into nematodes could inhibit the expression of sequence homologous genes, and confirmed that this inhibition mainly acts after transcription, so it is also called RNAi It is post-transcriptional gene silencin...

Claims

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