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Method for preparing culture environment purifying agent by spore fermentation waste liquid

A technology of fermentation waste liquid and purification agent, which is applied in the direction of chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of water eutrophication and environmental pollution, reduce manufacturing costs and improve water quality , The effect of reducing the cost of use

Inactive Publication Date: 2010-03-17
上海亘卓生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The spore fermentation waste liquid contains ammonia nitrogen, phosphorus and other substances produced by fermentation. The residual bacillus in this product is a d

Method used

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  • Method for preparing culture environment purifying agent by spore fermentation waste liquid
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Examples

Experimental program
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Effect test

Embodiment 1

[0019] A method for preparing a breeding environment purifier by using spore fermentation waste liquid, the specific steps are:

[0020] The first step: the composite bacteria are cultured in the medium at 20°C for 100 hours to obtain the composite bacteria; the composite bacteria are composed of 200 million / ml Saccharomyces cerevisiae 25wt%, 500 million / ml Lactobacillus plantarum 30wt%, 500 million / ml milk Streptococcus 40wt% and Rhodopseudomonas palustris 0.6 billion / ml 5wt%; the weight ratio of the compound strain to the culture medium is 1:99.

[0021] The medium in the first step consists of molasses 1wt%, peptone 0.4wt%, sodium chloride 0.2wt%, iron sulfate 30.05wt%, magnesium sulfate 0.02wt%, growth factor solution (containing vitamin H 2wt% and vitamin B 12wt% aqueous solution) 1wt% and the balance of water, pH is 6.0.

[0022] The second step: the spore fermentation waste liquid produced by the production of lichens or subtilis spore microbial feed additives is centr...

Embodiment 2

[0024] A method for preparing a breeding environment purifier by using spore fermentation waste liquid, the specific steps are:

[0025] The first step: culture the composite bacteria in the culture medium at 35°C for 150 hours to obtain the composite bacteria; the composite bacteria are composed of 200 million / ml Saccharomyces cerevisiae 20wt%, 500 million / ml Lactobacillus plantarum 35wt%, 500 million / ml milk Streptococcus 44wt% and 060 million / ml Rhodopseudomonas palustris 1wt%; the weight ratio of the compound strain to the culture medium is 3:97.

[0026] The medium in the first step consists of molasses 1wt%, peptone 0.4wt%, sodium chloride 0.2wt%, iron sulfate 30.05wt%, magnesium sulfate 0.02wt%, growth factor solution (containing vitamin H 2wt% and vitamin B 12wt% aqueous solution) 1wt% and the balance of water, pH is 6.0.

[0027] The second step: the spore fermentation waste liquid produced by the production of lichens or subtilis spore microbial feed additives is ce...

Embodiment 3

[0029] A method for preparing a water purifying agent by using spore fermentation waste liquid, the specific steps are:

[0030] The first step: the composite bacteria are cultured in the culture medium at 30°C for 120 hours to obtain the composite bacteria; the composite bacteria are composed of 200 million / ml Saccharomyces cerevisiae 25wt%, 500 million / ml Lactobacillus plantarum 30wt%, 500 million / ml milk Streptococcus 40wt% and Rhodopseudomonas palustris 0.6 billion / ml 5wt%; the weight ratio of the compound strain to the culture medium is 10:90.

[0031] The medium in the first step consists of molasses 1wt%, peptone 0.4wt%, sodium chloride 0.2wt%, iron sulfate 30.05wt%, magnesium sulfate 0.02wt%, growth factor solution (containing vitamin H 2wt% and vitamin B 12wt% aqueous solution) 1wt% and the balance of water, pH is 6.0.

[0032] The second step: the spore fermentation waste liquid produced by the production of lichens or subtilis spore microbial feed additives is cent...

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Abstract

The invention relates to a method for preparing culture environment purifying agent by spore fermentation waste liquid, which is characterized in that the method comprises the following specific steps: 1. culturing compound strains to obtain compound bacteria, wherein the compound strains are composed of saccharomyces cerevisiae, lactobacillus plantarum, 5 hundred million/ml streptococcus lactis and rhodopseudomonas palustris; 2. decentralizing spore fermentation waste liquid produced when preparing lichens or litter spore microbiological feed additive, adding 5-10wt% of molasses into the obtained supernatant, then inoculating 1-10wt% of compound bacteria, and fermenting in a sealed environment at the temperature of 20-35 DEG C to obtain water body purifying agent. The method of the invention not only can obtain the purposes of reducing emission and protecting environment, but also can fully utilize nutrient components in waste liquid to produce culture environment modifier, thus saving cost and water resource. The invention has the outstanding characteristics of simple and easily realized operation, low operation cost and the like and is easy for industrial application.

Description

technical field [0001] The invention relates to a method for preparing a breeding environment purifier by using spore fermentation waste liquid, and belongs to the technical field of environmental improvement and environmental protection. Background technique [0002] Bacillus is considered to be able to secrete various enzymes such as protease. Decompose a large amount of residual bait, excrement waste, animal and plant residues, and harmful gases such as ammonia and hydrogen sulfide accumulated in the breeding environment into small molecular polypeptides, high-level fatty acids, etc., and then decompose into smaller molecular organic substances such as amino acids, low-level fatty acids, and simple Sugars, cyclic hydrocarbons, etc. are finally decomposed into carbon dioxide, nitrates, sulfates, etc., which can reduce the pollution of livestock and poultry pens, eliminate the odor of pens, and help create a good and comfortable breeding environment. When used in aquacultu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/865C12R1/25C12R1/46C12R1/01
Inventor 江瀚王戌晋
Owner 上海亘卓生物工程有限公司
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