A detection method for insertion mutation polymorphism of cattle npm1 gene
A technology of insertion mutation and polymorphism, applied in the field of molecular genetics, can solve problems such as lack of research and blanks
Inactive Publication Date: 2011-12-28
NORTHWEST A & F UNIV
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Problems solved by technology
At present, there is a lack of research in the field of genetic variation of the NPM1 gene in local yellow cattle in China. The functional research of this gene locus and its genetic variation and economic traits (such as: birth weight, body weight, daily gain, body height, body oblique length, chest circumference, ischial end width and other traits) research on the association is still blank
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[0029] The present invention firstly amplifies the NPM1 gene of Chinese local cattle populations by PCR, and uses DNA sequencing technology to screen for 12bp insertion mutations in the coding region closely related to cattle economic traits; aiming at the gene polymorphisms caused by the mutations, PAGE electrophoresis method is then used Detection and identification of the 12bp insertion mutation polymorphism in the coding region of the Chinese local cattle NPM1 gene, and according to the results of the correlation analysis between the different genotypes of the individual cattle and their corresponding generation trait data, the individuals with the 12bp insertion mutation in the coding region of the cattle NPM1 gene are eliminated in advance , To speed up the establishment of cattle populations with high-quality economic traits. The present invention is described in further detail below, which is an explanation rather than a limitation of the present invention.
[0030] a. Sc...
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The invention discloses a method for detecting the insertion mutation polymorphism of the cattle NPM1 gene, which comprises the following steps: 1) using the cattle genome to be tested containing the NPM1 gene as a template, and using primer P as a primer to carry out PCR amplification; The amplified fragments were detected by polyacrylamide gel electrophoresis, and EB staining was performed after 200V voltage electrophoresis for 50-60 min; 3) The insertion mutation polymorphism of NPM1 gene was typed according to the detection results of polyacrylamide gel electrophoresis. The invention provides a method for detecting the polymorphism of the cattle NPM1 gene associated with economic traits, aiming at the gene polymorphism in which the 12 bp insertion mutation in the coding region of the NPM1 gene leads to a change in the conformation of the encoded protein, by setting specific primers for PCR After amplification, the genotype was typed according to the results of polyacrylamide gel electrophoresis, which provided technical support for detecting the genetic variation of the NPM1 gene of Chinese local cattle.
Description
Technical field [0001] The invention belongs to the field of molecular genetics, and relates to the detection of gene insertion mutation polymorphism, in particular to a 12bp insertion mutation polymorphism of cattle NPM1 gene and a detection method thereof. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, which include base substitutions, insertions, deletions, and duplicate sequence copies Number of changes. [0003] Single nucleotide polymorphism refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence, which is mainly caused by the conversion or transversion of a single base. SNPs with conversion variants accounted for about 2 / 3, and other SNPs were at similar levels. The cytosine of CpG dinucleotide is the most prone to ...
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IPC IPC(8): C12Q1/68G01N27/447
Inventor 陈宏黄永震蓝贤勇淮永涛李转见胡沈荣雷初朝
Owner NORTHWEST A & F UNIV