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Whole clearing method

A transparent and integral technology, applied in the preparation of test samples, etc., can solve the problems that integral dyeing and transparent techniques cannot be used directly, soaking, long time for transparency, and many processing procedures for film production, so as to avoid shrinkage and simple method Ease of operation and simplification of processing procedures

Inactive Publication Date: 2010-03-17
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most commonly used transparent agent in the overall transparent technology is wintergreen oil. Due to the penetration and transparency of plant materials by wintergreen oil, it needs to be carried out in an anhydrous environment. shrink
At the same time, it takes a long time for the material to be soaked and transparent by the oil transparent agent, which makes the production process more, and the total production time is longer
In addition, for materials preserved in ethanol solution in the field, the color often becomes darker, making the overall dyeing and transparent technology unable to be used directly

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] Isolation of Young Tender Seeds of Stephania vine

[0032] First put the glue block in the field of view of the dissecting microscope (refer to the paper published by Hong Yaping on "Anhui Agricultural Science": the method of using the glue block to observe the flower shape of the plant under the dissecting microscope), and use tweezers under the dissecting microscope to remove the steppaw The young fruit is picked from the young infructome and placed on the glue block. Clamp both sides of the young fruit with pointed tweezers, and use a dissecting needle to cut the exocarp and mesocarp along the dorsal and ventral sutures of the young fruit. Use tweezers and a dissecting needle to break apart the young fruit along the cut dorsal suture, and use a dissecting needle to gently separate the young and tender seeds from the ovary chamber. The separated young and tender seeds are as follows: figure 1 .

Embodiment 2

[0034] Isolation of sorghum ovules

[0035] Pick off the floret of sorghum from the spikelet, stick it on the glue block, separate the ovary with a dissecting needle and tweezers, stick it on the glue block, and separate the ovule with a dissecting needle, see figure 2 .

[0036] Example 1

[0037] A holistic transparency approach includes the following steps:

[0038] A. Separation of part of the plant material to be observed;

[0039] B, soak the isolated plant material in 84 disinfectant (purchased from Luoyang Jiekang Disinfectant Factory), until the plant material becomes transparent;

[0040] C. Take a 0.5×0.7mm filter paper piece, put it into the 84 disinfectant to absorb the transparent plant material, then transfer the filter paper with the plant material absorbed into the groove of the concave glass slide, add 84 disinfectant dropwise to make The plant material is detached from the surface of the filter paper, the filter paper is removed, the plant material ente...

Embodiment 3

[0047] A holistic transparency approach includes the following steps:

[0048] Separation of the part of the plant material to be observed;

[0049] Soak the isolated plant material in 84 disinfectant (purchased from Luoyang Jiekang Disinfectant Factory) until the plant material becomes transparent;

[0050] Take a 0.6×0.7mm filter paper piece, put it into the 84 disinfectant to absorb the transparent plant material, then transfer the filter paper piece with the plant material absorbed to clean water for soaking and rinsing to remove the residue on the plant material; then put the plant material Use a filter paper piece to transfer to the gardenia pigment solution for soaking and dyeing. When the plant material is dyed a darker color, use the filter paper piece to take it out and transfer it to the groove of the concave glass slide, and add water dropwise to make the plant material separate from the surface of the filter paper. , remove the filter paper, make the plant material...

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Abstract

The invention discloses a whole clearing method. The method comprises the following sequential steps: A. separating plant material from a part to be observed; B. soaking the plant material in 84 disinfectant till the plant material is transparent; and C. taking out the plant material for flaking observation. The method has the advantages that 1. clearing reagent and staining solution which are used in the method are market products, convenient to buy and cheap; 2. when the clearing reagent is used for clearing treatment, no material dehydration and the like is needed before the treatment, thereby not only avoiding problems such as shrinkage and the like caused by the material dehydration but also greatly shortening the time for clearing and simplifying the flaking treatment process; 3. asfor field-use material preserved by ethanol solution, the method can be directly used for achieving a relatively good observation effect; 4. the method for using filter paper to take out of the plantmaterial is simple and easy to operate, and the material is easily available; and 5. lens paper is used for fixing the plant material so that the method for dissecting the plant material under an anatomical lens is simple to operation and the material is easily available.

Description

technical field [0001] The invention relates to an overall transparent method, which belongs to the field of plant microscopy technology. Background technique [0002] The overall transparent technology is to use a transparent agent to soak the plant material, or to treat the plant material with some dyes or chemical reaction substances and a transparent agent, so that the optical properties of the material will be changed, and the light transmission will be enhanced, so that it can be processed without dissection. The internal structural features of the material were observed. The existing overall transparent technology mostly adopts to dye the plant material first, and then change the optical and light transmission characteristics of the material by dehydrating and soaking the plant material. At present, the most commonly used transparent agent in the overall transparent technology is wintergreen oil. Due to the penetration and transparency of plant materials by wintergre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 洪亚平李友军仝克勤张有福韩赞平
Owner HENAN UNIV OF SCI & TECH
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