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Liquid chromatogram measuring method for total content of brassin

A technology of liquid chromatography and determination method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem that the content of total brassin can not be accurately determined, etc.

Inactive Publication Date: 2010-03-17
蒋捷先
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiency that the total brassinolide content in the existing brassinolides cannot be accurately measured, the present invention provides a method to determine the amount of the remaining derivatization reagent after the derivatization of the brassinolates by liquid chromatography. Quantitative method for the total amount of orcinoids

Method used

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  • Liquid chromatogram measuring method for total content of brassin
  • Liquid chromatogram measuring method for total content of brassin
  • Liquid chromatogram measuring method for total content of brassin

Examples

Experimental program
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Embodiment approach 1

[0058] Instruments: Shimadzu LC-6A high performance liquid chromatograph; 7125 manual injector; C-R3A ultraviolet-visible light detector; data processor;

[0059]Reagent: methanol (chromatographic reagent); brassinolide standard substance is brassinolide (BR) standard substance; phenylboronic acid standard substance is phenylboronic acid standard substance, natural brassinolide substance and preparation;

[0060] Chromatographic conditions:

[0061] Chromatographic column: 6.0mm I.d. × 150mm stainless steel column, built-in filler;

[0062] The mobile phase composition volume ratio: methanol: water = 80: 20; the mobile phase is used after being filtered through a 0.5 μm filter membrane;

[0063] Flow rate: 0.7~1ml / min;

[0064] Detection wavelength: UV 222nm;

[0065] Column temperature: room temperature;

[0066] Sample processing: carry out according to the step in the summary of the invention:

[0067] According to the preparation method of sample processing (1) phen...

Embodiment approach 2

[0076] Instrument, chromatographic conditions are the same as embodiment one;

[0077] Reagent: methanol (chromatographic reagent); brassinolide standard substance is epibrasinolide (ep-BR) standard substance; phenylboronic acid substance standard substance is phenylboronic acid standard substance, natural brassinolide substance and preparation ;

[0078] Sample processing: carry out according to the step in the summary of the invention:

[0079] According to the preparation method of sample processing (1) phenylboronic acid standard substance solution, make phenylboronic acid standard substance solution for subsequent use;

[0080] According to the preparation method of sample processing (2) brassinolide standard substance solution, accurately weigh 15.0mgep-BR standard substance and accurately weigh 10.0mg phenylboronic acid standard substance, put them into a beaker together, after dissolving with methanol, Transfer to a 25ml volumetric flask, react in a water bath at 80°...

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Abstract

The invention discloses a liquid chromatogram measuring method for the total content of brassin, belonging to the technical field of chromatographic analysis and detection, and comprising the following steps: preparation of phenylboronic acid substance standard solution, preparation of brassin substance standard solution and preparation of natural brassin substance sample solution. Under proper conditions, phenylboronic acid substance has derivatization reaction with all brassins having o-hydroxyl. The liquid chromatogram measuring method well achieves the measurement of the total content of brassin in the brassin substance extracted from natural substance, and has great meaning and value in the scientific guidance of research, production and application. The liquid chromatogram measuringmethod is suitable for measurement of the total content of brassin in the brassin substance extracted from natural substance.

Description

Technical field [0001] The invention relates to a liquid chromatography determination method for the content of a plant growth regulator, in particular to a liquid chromatography determination method for brassinolide, and belongs to the technical field of chromatographic analysis and detection. Background technique [0002] At present, brassinolides are plant growth regulators, which can promote plant cell elongation, division, increase seed setting rate and comprehensively regulate plant growth and development. It has obvious effect of increasing production of grain, cotton, oil, hemp, fruit, vegetables and edible fungus, and has been widely used in agricultural production both at home and abroad. Because of its natural origin, brassinolides have the advantages of high efficiency, non-toxicity and pollution-free, which will be the development direction of green pesticides in the future. [0003] Brassinolides extracted from natural substances are a class of substances. At ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/28G01N30/86
Inventor 蒋捷先
Owner 蒋捷先